Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   ACFVPM_RS27760 Genome accession   NZ_CP171271
Coordinates   5946911..5949130 (+) Length   739 a.a.
NCBI ID   WP_003103282.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain W220606     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5941911..5954130
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFVPM_RS27740 - 5942886..5944154 (-) 1269 WP_003103278.1 malic enzyme -
  ACFVPM_RS27745 - 5944257..5945696 (-) 1440 WP_003095844.1 M48 family metalloprotease -
  ACFVPM_RS27750 - 5945693..5946460 (-) 768 WP_003103280.1 thermonuclease family protein -
  ACFVPM_RS27755 rpmE 5946520..5946735 (-) 216 WP_003095846.1 50S ribosomal protein L31 -
  ACFVPM_RS27760 priA 5946911..5949130 (+) 2220 WP_003103282.1 primosomal protein N' Machinery gene
  ACFVPM_RS27765 argS 5949380..5951143 (+) 1764 WP_003110892.1 arginine--tRNA ligase -
  ACFVPM_RS27770 - 5951178..5951873 (+) 696 WP_003095850.1 SPOR domain-containing protein -
  ACFVPM_RS27775 hslV 5951992..5952525 (+) 534 WP_003095852.1 ATP-dependent protease subunit HslV -
  ACFVPM_RS27780 hslU 5952554..5953897 (+) 1344 WP_003095854.1 ATP-dependent protease ATPase subunit HslU -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 82137.65 Da        Isoelectric Point: 8.9189

>NTDB_id=946047 ACFVPM_RS27760 WP_003103282.1 5946911..5949130(+) (priA) [Pseudomonas aeruginosa strain W220606]
MSDLILRLALPSPLRRLFDYRAPRGIPRSALQPGIRLLLPFGRRELVGVLIEVTDRSEVPEDKLKPALRVLDAKPPMPAH
LLELCRWTAQYYQHSLGDTLSWALPNLLRQGEPAEARQQRFWHATAQSSLDDPRLARAPRQRQALAILKQHPHGVSHELL
NQLQINKDSLDLLKEKGLVELEVRRHSTPPREGGWLAQAELPLNPEQRAAFEAVRASHGGFHCFLLAGVTGSGKTEVYLQ
LIRETLAAGRQALVLIPEINLGPQTLARFERRFNARIALLHSALTDRERLDAWLAARDGEADIVIGTRSALFTPLKRPGL
IIVDEEHDASYKQQDGLRYHARDLALVRARLENVPILLGSATPALESLHNAQAGRYGLLRLTQRAGGAHPPKFIRLDVKS
MPLDAGLSRPLQQAIGDTLAAGQQVLVFLNRRGFAPTLLCHDCGWISQCPRCDARMTVHQGSGELRCHHCDHRQRPPMNC
PQCGKLDLRPVGAGTERAEERLRILFPNHPVLRIDRDSTSRKHAMRDLFATINSGEPCILVGTQMLAKGHHFPRVTLVAI
LDADGGLFSADFRASERMAQQIVQVAGRAGRAEEPGRVLIQTHLADHPLLVQLTEDGYFAFAEQALSERRAAGLPPFAHL
ALLRAEAHKPGQAEAFLDSACSAAEQLLEQMGGPEVELLGPVPAPMERRAGKHRAQLLLQCMSRAPLHRLLTPWLQSLEQ
LPGGRQVRWSLDIDPIDLF

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=946047 ACFVPM_RS27760 WP_003103282.1 5946911..5949130(+) (priA) [Pseudomonas aeruginosa strain W220606]
GTGTCAGACCTGATCCTACGCCTAGCCCTGCCTTCGCCACTCCGCCGCCTGTTCGACTACCGCGCGCCCCGCGGCATTCC
GCGCAGCGCCCTACAGCCGGGGATTCGCCTGCTGCTGCCGTTCGGGCGCCGGGAGCTGGTTGGCGTTCTGATAGAGGTGA
CGGACCGTTCCGAAGTACCCGAAGACAAGCTCAAGCCTGCCCTCAGGGTACTCGACGCCAAGCCGCCGATGCCGGCGCAC
CTGCTCGAACTCTGTCGCTGGACCGCCCAGTACTACCAGCACAGCCTCGGCGACACCCTGTCCTGGGCGCTGCCCAACCT
GTTGCGCCAGGGCGAGCCGGCCGAGGCGCGCCAGCAGCGCTTCTGGCATGCCACCGCGCAATCCAGCCTCGACGATCCGC
GCCTGGCCCGGGCGCCGCGCCAGCGCCAGGCCCTGGCGATTCTCAAGCAGCATCCGCACGGGGTGTCCCATGAGTTGCTG
AACCAGTTGCAGATAAACAAGGACAGCCTCGACCTGCTCAAGGAAAAAGGCCTGGTCGAACTGGAGGTGCGGCGCCACAG
CACCCCGCCGCGCGAAGGCGGCTGGCTGGCCCAGGCGGAGTTGCCGCTGAACCCCGAGCAGCGCGCCGCCTTCGAGGCGG
TTCGCGCCAGCCACGGCGGTTTCCATTGCTTCCTGCTGGCCGGGGTCACCGGCAGCGGCAAGACCGAGGTCTACCTGCAA
CTGATCCGCGAAACCCTGGCCGCAGGCCGCCAGGCCCTGGTGCTGATTCCCGAGATCAACCTGGGGCCGCAGACCCTGGC
GCGTTTCGAACGCCGCTTCAACGCGCGCATCGCCCTGCTGCATTCGGCCCTGACCGACCGCGAGCGCCTCGACGCCTGGC
TCGCCGCCCGGGATGGCGAGGCCGACATCGTCATCGGCACCCGCTCGGCACTGTTCACGCCGCTGAAGCGGCCGGGACTG
ATCATCGTCGACGAGGAACACGACGCCTCCTATAAACAGCAGGACGGACTGCGCTACCACGCCCGCGACCTGGCACTGGT
ACGCGCACGCCTGGAGAACGTGCCGATCCTGCTCGGTTCGGCCACGCCCGCCCTGGAAAGCCTGCACAACGCCCAGGCCG
GCCGCTACGGCCTGCTGCGCCTGACCCAGCGCGCCGGTGGCGCGCACCCGCCGAAATTCATCCGCCTCGACGTGAAGAGC
ATGCCGCTCGACGCCGGCCTTTCCCGCCCGCTGCAACAGGCCATCGGCGATACCCTGGCGGCCGGCCAGCAGGTCCTGGT
GTTCCTCAACCGCCGCGGCTTCGCTCCGACCCTGCTTTGCCACGACTGCGGCTGGATCAGCCAATGCCCGCGCTGCGACG
CGCGGATGACCGTGCACCAGGGCAGCGGCGAGTTGCGCTGCCACCACTGCGACCACCGCCAGCGGCCGCCGATGAACTGC
CCGCAGTGCGGCAAGCTCGACCTGCGTCCGGTAGGCGCCGGCACCGAGCGCGCCGAGGAACGCCTGCGGATTCTCTTCCC
GAACCATCCGGTGCTGCGCATCGACCGCGACAGCACCTCGCGCAAGCACGCCATGCGCGACCTCTTCGCCACCATCAACA
GCGGTGAGCCATGCATCCTGGTCGGCACCCAGATGCTCGCCAAGGGGCACCACTTCCCACGGGTCACCCTGGTGGCGATC
CTCGATGCCGATGGCGGCCTGTTTTCCGCCGACTTCCGCGCCAGCGAACGCATGGCCCAGCAGATCGTCCAGGTCGCCGG
CCGCGCAGGACGCGCCGAGGAGCCCGGACGGGTACTGATCCAGACCCACCTGGCCGATCACCCGCTGCTGGTCCAGCTCA
CCGAGGACGGCTACTTCGCCTTTGCCGAACAGGCCCTCAGCGAACGCCGCGCCGCCGGCCTGCCGCCCTTCGCCCACCTC
GCCCTGCTGCGCGCCGAAGCGCACAAGCCGGGCCAGGCGGAAGCCTTCCTCGACAGCGCCTGCAGTGCCGCCGAGCAATT
GCTCGAGCAGATGGGCGGACCGGAGGTGGAACTGCTCGGGCCGGTGCCCGCGCCCATGGAGCGGCGTGCCGGCAAGCACC
GCGCGCAACTGCTGCTGCAGTGCATGAGCCGGGCGCCGCTGCATCGCCTGCTGACGCCCTGGCTGCAGAGCCTCGAACAG
TTGCCCGGCGGGCGCCAGGTGCGCTGGTCGCTCGATATCGATCCGATCGACCTGTTCTGA

Domains

Predicted by InterProScan.

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)

Primosomal protein N C-terminal domain (PriA_C)

(636-735)

Helicase conserved C-terminal domain (Helicase_C)

(522-592)

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)

DEAD/DEAH box helicase (DEAD)

(207-367)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

47.613

99.188

0.472