Detailed information NTG0945753

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: ACF0HW_RS16905
• Genome accession: NZ_CP171208
• Coordinates: 3433363..3434484 (-)
• Length: 373 a.a.
• NCBI ID: WP_011996281.1
• Uniprot ID: A7Z131
• Organism: Bacillus amyloliquefaciens strain HN11
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3428363..3439484

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACF0HW_RS16885	-	3428810..3429505 (-)	696	WP_011996285.1	metal ABC transporter ATP-binding protein	-
ACF0HW_RS16890	-	3429560..3430519 (-)	960	WP_011996284.1	metal ABC transporter substrate-binding protein	-
ACF0HW_RS16895	-	3430705..3432384 (-)	1680	WP_011996283.1	alpha-glucosidase	-
ACF0HW_RS16900	-	3432468..3433247 (-)	780	WP_011996282.1	glucose 1-dehydrogenase	-
ACF0HW_RS16905	rapF	3433363..3434484 (-)	1122	WP_011996281.1	Rap family tetratricopeptide repeat protein	Regulator
ACF0HW_RS16910	-	3434594..3435097 (+)	504	WP_011996280.1	M15 family metallopeptidase	-
ACF0HW_RS16915	-	3435094..3436452 (-)	1359	WP_023356694.1	YcdB/YcdC domain-containing protein	-
ACF0HW_RS16920	-	3436653..3438053 (-)	1401	WP_041482174.1	YcdB/YcdC domain-containing protein	-
ACF0HW_RS16925	-	3438180..3439175 (+)	996	WP_007409036.1	DUF5105 domain-containing protein	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44317.53 Da
• Isoelectric Point: 5.0806

>NTDB_id=945753 ACF0HW_RS16905 WP_011996281.1 3433363..3434484(-) (rapF) [Bacillus amyloliquefaciens strain HN11]
MKSKIPSEEVAVKMNEWYKLIRAFEADQAEALKREIQYELEDMEENQDLLLYFSLMEFRHRIMLDKLKPMKDGDCQPPFS
EMLTEIEMNQQKLTGLLEYYFYYFRGMYEFKQKNFISAISHYKRAEEKLEYVEDDIEKAEFLYKISEVYYHIKQTFFSMN
YAGQALDIYKKYELYGMRRVQCKFIIAGNLMDAQQHKKALEYLECALEHAERLDDQYMIAAAFYNIGHCMYSLGDFCKAA
EDFERAAAGFEAHSFRQVIQAVFSLTHIYFKTEKYSEAAASCARGMRSAAEWEDEIYMTKFRLIKELYQGEGGQDSLKEY
FDILESKELLADTEELLQDAAHFFHKRENYKAASYFFSRLVGVQKRLQMNNYA

Nucleotide

• Length: 1122 bp

>NTDB_id=945753 ACF0HW_RS16905 WP_011996281.1 3433363..3434484(-) (rapF) [Bacillus amyloliquefaciens strain HN11]
ATGAAATCCAAGATTCCATCTGAAGAAGTGGCCGTAAAGATGAATGAATGGTATAAGCTGATCCGCGCGTTTGAAGCCGA
TCAAGCTGAAGCGCTGAAGCGGGAGATTCAATATGAACTCGAAGATATGGAAGAGAACCAGGATTTATTGTTGTATTTTT
CATTGATGGAATTTCGGCACCGCATCATGCTGGATAAATTAAAACCCATGAAAGACGGCGACTGCCAGCCTCCGTTCTCT
GAAATGCTCACTGAGATTGAGATGAACCAGCAAAAGCTGACCGGCTTACTGGAATATTATTTTTACTATTTCAGGGGAAT
GTATGAATTTAAGCAGAAAAACTTCATCTCAGCGATTTCTCATTATAAGCGGGCTGAGGAAAAGCTTGAATATGTGGAAG
ATGACATAGAGAAAGCCGAGTTTCTGTATAAAATTTCTGAGGTGTACTATCATATCAAGCAGACTTTCTTTTCGATGAAT
TACGCGGGGCAGGCGCTTGATATTTACAAGAAATATGAATTATACGGAATGAGAAGGGTGCAGTGCAAATTTATCATTGC
CGGAAATCTGATGGACGCCCAGCAGCATAAAAAAGCGCTGGAATACCTCGAATGCGCCTTGGAACACGCGGAGCGGCTTG
ATGATCAATATATGATTGCTGCGGCCTTTTATAATATCGGGCACTGTATGTACAGTCTGGGTGACTTTTGCAAGGCGGCG
GAGGATTTTGAACGGGCGGCTGCCGGATTTGAAGCACACAGCTTCCGGCAGGTCATCCAGGCTGTTTTTTCACTCACGCA
TATCTACTTTAAAACGGAAAAATACAGTGAAGCCGCCGCCAGCTGCGCGCGGGGCATGAGAAGCGCGGCGGAGTGGGAAG
ATGAGATCTATATGACGAAATTCCGTCTGATTAAAGAGCTTTATCAGGGGGAAGGCGGACAGGATTCGCTCAAAGAGTAT
TTTGACATTCTGGAATCAAAAGAGCTGTTGGCTGACACAGAGGAACTGCTTCAGGACGCGGCTCATTTCTTTCATAAACG
GGAGAACTACAAAGCGGCATCTTATTTTTTCAGCAGGCTTGTCGGCGTGCAAAAAAGGCTGCAAATGAACAACTACGCAT
AA

Domains

Predicted by InterProScan.

• Tetratricopeptide repeat (TPR_12) (187-251)

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

3D structure

• AlphaFold DB: A7Z131

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.588	99.464	0.424
rapC	Bacillus subtilis subsp. subtilis str. 168	40.054	99.732	0.399