Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   ACF0HW_RS08415 Genome accession   NZ_CP171208
Coordinates   1645555..1646691 (-) Length   378 a.a.
NCBI ID   WP_012117791.1    Uniprot ID   A7Z5Q9
Organism   Bacillus amyloliquefaciens strain HN11     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 1640555..1651691
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF0HW_RS08390 - 1641065..1641711 (+) 647 Protein_1645 response regulator -
  ACF0HW_RS08395 - 1641890..1643089 (+) 1200 WP_012117794.1 FAD/NAD(P)-binding oxidoreductase -
  ACF0HW_RS08400 - 1643248..1644660 (+) 1413 WP_012117793.1 rhodanese-like domain-containing protein -
  ACF0HW_RS08405 - 1644723..1645352 (+) 630 Protein_1648 UV damage repair protein UvrX -
  ACF0HW_RS08410 - 1645383..1645565 (-) 183 WP_041482200.1 hypothetical protein -
  ACF0HW_RS08415 rapF 1645555..1646691 (-) 1137 WP_012117791.1 Rap family tetratricopeptide repeat protein Regulator
  ACF0HW_RS08420 - 1646961..1647413 (+) 453 WP_012117790.1 hypothetical protein -
  ACF0HW_RS08425 - 1647552..1647630 (+) 79 Protein_1652 hypothetical protein -
  ACF0HW_RS08430 - 1647647..1648021 (-) 375 WP_007407921.1 YoqO family protein -
  ACF0HW_RS08435 - 1648147..1648383 (-) 237 WP_077411073.1 hypothetical protein -
  ACF0HW_RS08440 - 1648925..1649182 (+) 258 WP_023356967.1 DUF3221 domain-containing protein -
  ACF0HW_RS08445 sprB 1649544..1649720 (+) 177 WP_003153662.1 recombination directionality factor SprB -
  ACF0HW_RS08450 - 1649754..1650179 (-) 426 WP_003153663.1 SDR family NAD(P)-dependent oxidoreductase -
  ACF0HW_RS08455 phyC 1650495..1651646 (+) 1152 WP_012117785.1 3-phytase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 44525.17 Da        Isoelectric Point: 5.0394

>NTDB_id=945684 ACF0HW_RS08415 WP_012117791.1 1645555..1646691(-) (rapF) [Bacillus amyloliquefaciens strain HN11]
MEQLISSSKVGLKINEWYKYIRLFSVADAEILKDEVEQEIEHMEEDQDLLLYYSLMCFRHQLMLDYLEPKTMNEERPKVS
DLLEKIESKQSDLKGILEYYFNFFHGMYEFEQYEYLKAISFYNRAEKKLAVVPDEIERAEFHYKVAEVYYHMKQTHMSMH
HIVQAIETYKAHETYSIRVIQCSFVIGLNYLDMEYPEKAIPHFMNALKQAQEIDMPRLIGSSLYNLGLCSFTTGNMEKAL
EYFKEGVSIYQSNSLHNSSRLLDILLMLAKTHFKLFNTVEGKQVCKQGLTLSKALNDDVLGKMFDFLMGLFVENDKDRLF
KILDYLESKAMLSDVEDLATDAAMYYNVNEDHKVATAFYEKVLYARKQIQRGDCLYEI

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=945684 ACF0HW_RS08415 WP_012117791.1 1645555..1646691(-) (rapF) [Bacillus amyloliquefaciens strain HN11]
TTGGAGCAATTGATCTCTTCTTCAAAAGTTGGCTTAAAAATCAATGAATGGTACAAATACATAAGATTGTTCAGTGTTGC
AGATGCAGAAATTCTAAAGGATGAAGTTGAACAAGAAATAGAGCATATGGAAGAAGATCAAGACTTATTGTTGTACTATT
CGCTCATGTGTTTTAGACATCAATTAATGCTTGATTATCTTGAACCAAAAACCATGAATGAAGAACGCCCAAAAGTTTCA
GACTTATTAGAAAAGATCGAAAGTAAACAAAGTGATCTAAAAGGAATTCTTGAATATTATTTTAACTTTTTTCATGGGAT
GTATGAATTTGAACAATACGAATATCTTAAGGCAATAAGTTTTTACAACCGAGCTGAAAAGAAGTTAGCCGTTGTCCCCG
ATGAAATAGAGCGAGCTGAATTTCACTATAAGGTAGCTGAAGTGTACTATCACATGAAGCAAACCCACATGTCTATGCAT
CATATCGTTCAAGCAATAGAAACCTACAAAGCGCACGAAACGTACTCAATAAGAGTAATCCAATGTTCATTCGTCATCGG
TCTGAATTATTTAGACATGGAGTATCCAGAAAAAGCAATACCGCATTTCATGAATGCATTAAAACAAGCACAAGAAATTG
ATATGCCACGGTTAATAGGTTCTTCTTTGTACAATCTCGGTTTATGCTCATTTACAACAGGAAATATGGAGAAAGCATTG
GAGTATTTTAAAGAAGGCGTCTCAATTTATCAAAGTAATTCTCTTCACAATTCTAGCCGACTACTAGACATACTTCTAAT
GTTGGCAAAAACGCACTTTAAATTGTTCAACACGGTTGAGGGAAAACAGGTTTGTAAACAAGGTCTCACGCTATCAAAGG
CGTTGAACGATGACGTATTAGGAAAAATGTTTGATTTTCTTATGGGTTTATTCGTTGAGAATGACAAAGACCGACTTTTT
AAAATCTTAGATTACTTAGAATCAAAAGCTATGCTTTCAGATGTGGAAGACCTAGCGACTGATGCAGCAATGTATTATAA
TGTAAATGAAGACCATAAAGTAGCTACCGCCTTTTATGAGAAAGTGCTTTATGCCCGAAAACAAATTCAGAGAGGGGATT
GTCTTTATGAAATTTAA

Domains

Predicted by InterProScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)

Tetratricopeptide repeat (TPR_12)

(196-252)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7Z5Q9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

45.669

100

0.46
  rapC Bacillus subtilis subsp. subtilis str. 168

45.767

100

0.458