Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACFXAC_RS16230 Genome accession   NZ_CP170720
Coordinates   3311778..3313088 (-) Length   436 a.a.
NCBI ID   WP_033575208.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain JP5006     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3306778..3318088
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFXAC_RS16210 (ACFXAC_16210) - 3307019..3307735 (-) 717 WP_053574052.1 (Fe-S)-binding protein -
  ACFXAC_RS16215 (ACFXAC_16215) - 3307918..3309108 (-) 1191 WP_053574051.1 PLP-dependent aminotransferase family protein -
  ACFXAC_RS16220 (ACFXAC_16220) - 3309139..3309858 (-) 720 WP_007407377.1 FadR/GntR family transcriptional regulator -
  ACFXAC_RS16225 (ACFXAC_16225) lutP 3310064..3311740 (+) 1677 WP_031378309.1 L-lactate permease LutP -
  ACFXAC_RS16230 (ACFXAC_16230) rpoN/rpoN1 3311778..3313088 (-) 1311 WP_033575208.1 RNA polymerase factor sigma-54 Machinery gene
  ACFXAC_RS16235 (ACFXAC_16235) yvfG 3313166..3313384 (+) 219 WP_003151570.1 protein YvfG -
  ACFXAC_RS16240 (ACFXAC_16240) - 3313398..3314363 (-) 966 WP_039253776.1 polysaccharide pyruvyl transferase family protein -
  ACFXAC_RS16245 (ACFXAC_16245) - 3314342..3315514 (-) 1173 WP_007407381.1 DegT/DnrJ/EryC1/StrS aminotransferase family protein -
  ACFXAC_RS16250 (ACFXAC_16250) - 3315519..3316166 (-) 648 WP_031378307.1 acetyltransferase -
  ACFXAC_RS16255 (ACFXAC_16255) - 3316163..3316771 (-) 609 WP_007407383.1 sugar transferase -

Sequence

Protein


Download         Length: 436 a.a.        Molecular weight: 49896.82 Da        Isoelectric Point: 8.9296

>NTDB_id=945394 ACFXAC_RS16230 WP_033575208.1 3311778..3313088(-) (rpoN/rpoN1) [Bacillus amyloliquefaciens strain JP5006]
MDIKLQQTQMLKPQLTQELKQAITLLGYNSAELAGYIDELSLENPLIERKETDTPPLSYHKTSKNRMKERDGSQQIRLTR
KTLQDSLKQQALDMKLSDDEKKIFNYMIHSLDSNGYLKEDIQAAADHVSASQEETEAVLKKLQSLEPAGIGARSLQECIL
LQLRRLPERNEQAEWIADAYFELFARKNWKALTLETGIPLKDIQDIADMISALDPRPGLRYAHEEPGFYVEPDIFITVKN
GIIQARLNSRSFPDIQLHTHYQPLLSAAGCRDTKNYLSAKYQEWRWLNRALSQRKQTITRIIGVLTERQESFFLTGKSGM
QPLTLREVAETLELHESTISRAIKGKYVQTPYGLYEMKTFFSAKAESSGDGGASNYAVKAHLEALVGKEDKKKPFSDQKL
ADLLHEQYDIRISRRTVAKYRDQLNIPPSSARKRYS

Nucleotide


Download         Length: 1311 bp        

>NTDB_id=945394 ACFXAC_RS16230 WP_033575208.1 3311778..3313088(-) (rpoN/rpoN1) [Bacillus amyloliquefaciens strain JP5006]
ATGGATATCAAACTTCAGCAAACACAAATGTTAAAGCCTCAATTAACGCAAGAGCTTAAACAGGCTATCACATTACTCGG
ATACAATTCCGCGGAACTGGCCGGATATATTGATGAGCTCTCACTGGAAAACCCTCTGATTGAACGAAAGGAAACAGACA
CGCCGCCACTATCTTACCATAAAACAAGTAAAAACAGGATGAAAGAACGGGACGGAAGCCAGCAGATCAGACTTACCCGC
AAAACGCTGCAGGATTCGCTCAAACAGCAAGCCTTAGATATGAAGCTGTCCGATGATGAGAAAAAGATTTTCAATTATAT
GATACATTCATTGGACTCAAACGGATATTTAAAAGAGGACATACAAGCTGCCGCCGACCATGTATCCGCTTCACAAGAGG
AGACGGAAGCGGTTCTGAAAAAGCTTCAATCCCTTGAGCCCGCGGGAATCGGCGCCCGGTCTTTGCAGGAATGCATCCTT
CTTCAGCTCCGCCGTCTTCCCGAGCGGAACGAACAGGCGGAATGGATTGCCGACGCCTATTTTGAGCTATTTGCAAGAAA
GAACTGGAAAGCGCTTACACTGGAAACGGGCATTCCGCTTAAAGACATTCAGGACATCGCTGACATGATCAGCGCCCTTG
ATCCGAGGCCGGGGCTCCGTTATGCGCATGAGGAGCCGGGTTTTTACGTGGAACCGGATATTTTCATCACAGTGAAAAAC
GGCATCATTCAAGCGCGGCTCAACAGCCGTTCGTTTCCTGATATCCAGCTTCATACCCATTATCAGCCGCTCTTGTCAGC
TGCCGGGTGCCGTGACACGAAAAATTATCTGTCGGCAAAATATCAGGAATGGCGCTGGCTGAACCGGGCTTTGTCGCAAA
GAAAACAAACGATCACGAGAATTATCGGCGTCCTGACTGAACGGCAGGAATCCTTTTTTCTCACAGGAAAAAGCGGAATG
CAGCCGCTGACCTTAAGAGAGGTGGCGGAAACGCTGGAGCTCCACGAATCAACGATCAGCCGGGCCATCAAAGGGAAGTA
CGTTCAGACGCCGTACGGACTGTATGAAATGAAAACATTTTTCTCGGCGAAAGCTGAATCGTCGGGGGACGGCGGCGCCT
CCAATTATGCCGTAAAAGCGCATCTGGAGGCATTGGTCGGTAAAGAAGATAAAAAGAAACCTTTTTCAGATCAAAAGCTT
GCCGATCTGCTGCATGAACAATATGACATCCGAATCTCAAGGCGCACCGTCGCCAAATACCGTGACCAATTAAATATTCC
GCCGTCATCGGCGAGAAAACGGTACAGCTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(275-434)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(77-261)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

33.4

100

0.385