Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   WAA16_RS11295 Genome accession   NZ_CP146984
Coordinates   2220375..2221445 (-) Length   356 a.a.
NCBI ID   WP_060596836.1    Uniprot ID   -
Organism   Bacillus pumilus strain S9     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2215375..2226445
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WAA16_RS11245 (WAA16_11245) - 2215469..2216041 (-) 573 WP_066031752.1 signal peptidase I -
  WAA16_RS11250 (WAA16_11250) tapA 2216093..2216620 (-) 528 WP_060596834.1 amyloid fiber anchoring/assembly protein TapA -
  WAA16_RS11255 (WAA16_11255) - 2216920..2217198 (+) 279 WP_223250494.1 DUF3889 domain-containing protein -
  WAA16_RS11260 (WAA16_11260) - 2217232..2217426 (-) 195 WP_024718846.1 YqzE family protein -
  WAA16_RS11265 (WAA16_11265) comGG 2217485..2217868 (-) 384 WP_034661288.1 competence type IV pilus minor pilin ComGG -
  WAA16_RS11270 (WAA16_11270) comGF 2217865..2218365 (-) 501 WP_034661289.1 competence type IV pilus minor pilin ComGF -
  WAA16_RS11275 (WAA16_11275) comGE 2218304..2218621 (-) 318 WP_034661291.1 competence type IV pilus minor pilin ComGE -
  WAA16_RS11280 (WAA16_11280) comGD 2218605..2219051 (-) 447 WP_066030666.1 competence type IV pilus minor pilin ComGD -
  WAA16_RS11285 (WAA16_11285) comGC 2219044..2219337 (-) 294 WP_106072454.1 competence type IV pilus major pilin ComGC Machinery gene
  WAA16_RS11290 (WAA16_11290) comGB 2219354..2220394 (-) 1041 WP_066030664.1 competence type IV pilus assembly protein ComGB -
  WAA16_RS11295 (WAA16_11295) comGA 2220375..2221445 (-) 1071 WP_060596836.1 competence type IV pilus ATPase ComGA Machinery gene
  WAA16_RS11300 (WAA16_11300) - 2221623..2222495 (-) 873 WP_034661297.1 STAS domain-containing protein -
  WAA16_RS11310 (WAA16_11310) - 2222719..2223099 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  WAA16_RS11315 (WAA16_11315) - 2223312..2223554 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  WAA16_RS11320 (WAA16_11320) - 2223603..2224241 (-) 639 WP_106156274.1 MBL fold metallo-hydrolase -
  WAA16_RS11325 (WAA16_11325) - 2224399..2224572 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  WAA16_RS11330 (WAA16_11330) - 2224597..2224911 (-) 315 WP_034662003.1 MTH1187 family thiamine-binding protein -
  WAA16_RS11335 (WAA16_11335) - 2224989..2226242 (-) 1254 WP_060596687.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40267.63 Da        Isoelectric Point: 9.0692

>NTDB_id=945308 WAA16_RS11295 WP_060596836.1 2220375..2221445(-) (comGA) [Bacillus pumilus strain S9]
MYGIEYLGQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRSGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASIRLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDLIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGETCQLYCKLNRSVRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=945308 WAA16_RS11295 WP_060596836.1 2220375..2221445(-) (comGA) [Bacillus pumilus strain S9]
TTGTATGGTATTGAATATTTGGGACAAGAGCTGCTAGAAGAAGCATGCCGCATGAGAGCATCTGATGTGCATATTGTTCC
AAAAGAAAAGGAAGCGTCGGTATCTTTCCGCGTAGACTCAGATTTAATTCAGCAGCGAACCATTGATAAAAAAAGCGGCG
AGCGGCTCATTGCTCATTTTAAATTTTTATCATCCATGGATATCGGGGAAAAAAGGAGGCCGCAAAATGGGTCACTAGCT
GTGATGCTAAGAAGCGGCCAGGTGTTTATTCGGATGTCTACCTTACCAACTGTAAATGATGAGAGTTTAGTGATTAGAAT
ATTGCCGCAGGATCATGTTCCAAAAATAAAACATCTGTCACTATTTCCGAAAGCCTCCATCAGATTATTATCATTTTTGA
ATCATTCGCATGGGCTGATTTTATTTACCGGTCCAACAAATTCAGGGAAAACAACAACCCTTTATTCATTAATCCAGTTT
GCAAAAAAGAATTTCAACCGAAATATTATTACACTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGTTACAGGTTCA
AGTGAATGAAAAAGCCGGCATCACATATGCCGCAGGATTACGTGCTATTTTAAGACATGATCCAGATCTGATTGTGCTAG
GAGAAATAAGAGATGCCGAAACGGCCCGAACGGCAATTAGAGCAGCACTGACAGGCCATTTAGTGATGAGTACGCTTCAT
GCGAAAAATGCAAAAGGAGCCCTTTATCGTATGCTTGAATTTGGTGTCACGATGAATGAACTCGAACAAACGATGGTTGC
GATTGCTGCGCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAACATGTCAGCTTTACTGTAAATTAAATCGAT
CGGTCAGACGAACAAATGTATTTGAACTGCTGTTCGGAAAGGAGCTAGGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGGAAAGGAGTGGCACTAGGCTATTTATCGAAAAACACCTATCA
TCGCTGGGTTTATGAAGAAGCAAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.124

99.438

0.638