Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ACFW98_RS02495 Genome accession   NZ_CP170719
Coordinates   582509..582943 (+) Length   144 a.a.
NCBI ID   WP_395096774.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain JP5008     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 577509..587943
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFW98_RS02490 (ACFW98_02490) - 581009..582220 (-) 1212 WP_395096773.1 cytochrome P450 -
  ACFW98_RS02495 (ACFW98_02495) nucA/comI 582509..582943 (+) 435 WP_395096774.1 sporulation protein Machinery gene
  ACFW98_RS02500 (ACFW98_02500) - 583003..583758 (+) 756 WP_003154084.1 YoaK family protein -
  ACFW98_RS02505 (ACFW98_02505) - 583792..584154 (-) 363 WP_395096776.1 hypothetical protein -
  ACFW98_RS02510 (ACFW98_02510) - 584347..585675 (-) 1329 WP_007410382.1 S8 family peptidase -
  ACFW98_RS02515 (ACFW98_02515) - 585854..586087 (+) 234 WP_015239897.1 hypothetical protein -
  ACFW98_RS02520 (ACFW98_02520) - 586343..587050 (+) 708 WP_007410381.1 poly-gamma-glutamate hydrolase family protein -
  ACFW98_RS02525 (ACFW98_02525) - 587110..587562 (+) 453 WP_014305039.1 OsmC family protein -
  ACFW98_RS02530 (ACFW98_02530) - 587576..587929 (-) 354 WP_007410379.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15504.45 Da        Isoelectric Point: 7.8747

>NTDB_id=945144 ACFW98_RS02495 WP_395096774.1 582509..582943(+) (nucA/comI) [Bacillus amyloliquefaciens strain JP5008]
MNAFMKWAASLLLVISLQFGFTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSGGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=945144 ACFW98_RS02495 WP_395096774.1 582509..582943(+) (nucA/comI) [Bacillus amyloliquefaciens strain JP5008]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCTTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCAGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
CGGGGCTGATATCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATATT
CCGGCGGCACGAGAGTATTATTTATCGTTCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

58.12

81.25

0.472