Detailed information    

insolico Bioinformatically predicted

Overview

Name   clpE   Type   Regulator
Locus tag   ACFW98_RS00695 Genome accession   NZ_CP170719
Coordinates   114432..116528 (-) Length   698 a.a.
NCBI ID   WP_225046232.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain JP5008     
Function   repress competence development (at the early growth phase) (predicted from homology)   
Competence regulation

Genomic Context

Location: 109432..121528
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFW98_RS00670 (ACFW98_00670) - 110021..110281 (+) 261 WP_003154690.1 aspartyl-phosphate phosphatase Spo0E family protein -
  ACFW98_RS00675 (ACFW98_00675) - 110492..112009 (-) 1518 WP_046559543.1 ATP-binding protein -
  ACFW98_RS00680 (ACFW98_00680) - 112208..112654 (+) 447 WP_015417342.1 MarR family winged helix-turn-helix transcriptional regulator -
  ACFW98_RS00685 (ACFW98_00685) motB 112690..113475 (-) 786 WP_003154685.1 flagellar motor protein MotB -
  ACFW98_RS00690 (ACFW98_00690) motA 113447..114262 (-) 816 WP_007408461.1 flagellar motor stator protein MotA -
  ACFW98_RS00695 (ACFW98_00695) clpE 114432..116528 (-) 2097 WP_225046232.1 ATP-dependent protease ATP-binding subunit ClpE Regulator
  ACFW98_RS00700 (ACFW98_00700) - 116931..117968 (+) 1038 WP_012117417.1 membrane protein -
  ACFW98_RS00705 (ACFW98_00705) - 118066..119183 (+) 1118 Protein_128 hypothetical protein -
  ACFW98_RS00710 (ACFW98_00710) queC 119435..120094 (+) 660 WP_003154679.1 7-cyano-7-deazaguanine synthase QueC -
  ACFW98_RS00715 (ACFW98_00715) queD 120095..120538 (+) 444 WP_007408455.1 6-carboxytetrahydropterin synthase QueD -
  ACFW98_RS00720 (ACFW98_00720) queE 120531..121262 (+) 732 WP_015239741.1 7-carboxy-7-deazaguanine synthase QueE -

Sequence

Protein


Download         Length: 698 a.a.        Molecular weight: 77760.77 Da        Isoelectric Point: 5.4314

>NTDB_id=945123 ACFW98_RS00695 WP_225046232.1 114432..116528(-) (clpE) [Bacillus amyloliquefaciens strain JP5008]
MRCQHCQKNEATIRLNMQVNSIQKQFVLCETCYTELTRKPSMSMGPQSFGFPFEQGFAPNQNKQQAGSEKGLLDELAQNV
TNGAKAGLIDPVIGRDHEVARVIEILNRRNKNNPVLIGEPGVGKTAIAEGLALKIAEGDVPNKLKNKELYLLDVASLVAN
TGIRGQFEERMKQLMNELKERKNVILFIDEIHLLVGAGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAALE
RRFQPVMVHEPTIEQAISILEGIKDKYEKYHGVTFSNEAINACVTLSSRYIQDRHLPDKAIDLLDEAGSKANLELDTVNE
EDAAERLKAIDAEKTKALHEENYELAAKLRDEEAALEKQLAHETPASSPEIGAEHIQAIIEQKTGIPVGKLQADEQVKMK
ELEEHLHQRVIGQEKAVKKVAKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKTLADELFGTKDSIIRLDMSEYMEK
HAVSKIIGSPPGYVGHDEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLTDSQGRTVSFKDTVLIMTSNA
GTGEKQVKVGFQSEESVLEEQTLIDSLSAFFKPEFLNRFDSIIEFQSLKKEHLVKIVSLLLTELEDTLKERGISLSVTDE
AKEKIAEIGYHPAFGARPLRRTIQELIEDEMTDLLLDNNDIKAFHVVMEEDGIKVRAQ

Nucleotide


Download         Length: 2097 bp        

>NTDB_id=945123 ACFW98_RS00695 WP_225046232.1 114432..116528(-) (clpE) [Bacillus amyloliquefaciens strain JP5008]
ATGCGTTGTCAACATTGTCAGAAAAACGAGGCGACGATTCGCCTGAACATGCAAGTCAATTCCATTCAAAAACAATTCGT
TTTATGTGAAACATGTTATACAGAATTAACCCGAAAACCGTCTATGAGCATGGGGCCGCAATCATTCGGATTCCCGTTTG
AGCAAGGTTTCGCTCCCAATCAAAATAAACAGCAGGCCGGCTCTGAAAAAGGCCTGCTCGACGAGCTCGCTCAAAACGTC
ACAAACGGCGCAAAAGCAGGTCTGATTGACCCTGTGATCGGCCGCGATCATGAAGTGGCGCGCGTAATTGAAATTTTGAA
CCGCCGGAATAAAAACAATCCGGTGCTGATCGGTGAACCGGGCGTCGGTAAAACGGCTATTGCAGAAGGCCTCGCGCTTA
AAATCGCTGAAGGCGATGTGCCGAACAAATTGAAAAACAAAGAGCTTTACCTGCTTGACGTCGCTTCTCTAGTCGCCAAT
ACAGGGATCAGAGGCCAGTTTGAAGAAAGAATGAAACAATTAATGAACGAATTAAAAGAACGGAAAAACGTCATCTTGTT
CATTGACGAGATTCACCTTCTTGTAGGCGCCGGCTCTGCCGAGGGATCAATGGATGCCGGGAACATCCTGAAACCGGCGC
TTGCCAGAGGCGAGCTGCAGGTCATCGGGGCGACAACACTGAAAGAGTACCGCCAGATTGAAAAAGATGCGGCGCTTGAA
AGACGCTTCCAGCCTGTCATGGTTCACGAGCCTACAATAGAACAAGCCATCAGCATTCTGGAAGGCATTAAAGATAAATA
TGAGAAATACCACGGCGTCACATTCAGCAATGAAGCGATAAACGCCTGTGTTACGCTTTCATCCCGTTACATTCAGGACC
GCCACCTGCCGGATAAAGCGATCGACTTATTAGATGAAGCCGGCTCTAAGGCGAACCTGGAGCTTGATACCGTAAATGAA
GAAGATGCGGCTGAGAGACTCAAAGCAATTGACGCGGAGAAAACAAAAGCGCTTCATGAAGAAAATTATGAGCTTGCCGC
GAAACTGCGTGATGAAGAAGCGGCGTTGGAAAAGCAGCTGGCGCATGAAACACCGGCCTCTTCACCTGAAATCGGGGCGG
AGCATATTCAAGCCATTATTGAACAGAAAACAGGCATTCCGGTCGGAAAACTCCAGGCTGATGAACAAGTTAAAATGAAG
GAACTTGAAGAGCATCTTCATCAGCGCGTCATCGGTCAGGAAAAAGCCGTGAAAAAAGTGGCGAAAGCGGTCAGACGAAG
CCGCGCCGGCTTAAAATCCAAAAACAGACCTGTCGGCTCCTTCCTCTTTGTCGGACCGACCGGAGTCGGAAAAACCGAGT
TATCAAAGACACTCGCAGATGAACTGTTCGGTACAAAAGATTCCATTATCCGTCTCGATATGAGTGAATACATGGAAAAA
CACGCCGTATCAAAAATCATCGGTTCACCGCCGGGATATGTCGGACACGATGAAGCCGGTCAATTAACGGAAAAAGTGCG
CCGCAATCCGTACAGCATCGTGCTGCTCGATGAGATTGAAAAAGCCCACCCTGATGTCCAGCATATGTTTCTGCAGATTA
TGGAGGACGGCCGTCTGACAGACAGCCAGGGCAGAACGGTCAGCTTCAAAGATACCGTTTTGATTATGACAAGCAACGCT
GGAACCGGCGAAAAACAGGTGAAAGTCGGATTCCAATCTGAAGAGAGCGTCCTGGAAGAACAAACGCTGATTGATTCACT
GAGCGCGTTCTTCAAACCGGAGTTTTTAAACCGGTTTGACAGCATTATTGAATTCCAATCCCTGAAAAAAGAACATCTCG
TGAAAATCGTCAGCCTGCTTCTCACAGAGCTTGAAGACACATTAAAAGAGCGCGGCATCAGCCTGAGCGTAACGGACGAA
GCGAAAGAAAAAATCGCTGAAATCGGCTACCACCCAGCTTTCGGAGCACGCCCGCTCAGAAGAACCATCCAAGAATTGAT
CGAGGACGAAATGACAGATCTCTTATTGGATAACAACGATATAAAAGCCTTCCATGTCGTAATGGAAGAGGACGGCATCA
AAGTCCGGGCACAATAA

Domains

Predicted by InterProScan.

C-terminal, D2-small domain, of ClpB protein (ClpB_D2-small)

(608-679)

AAA lid domain (AAA_lid_9)

(252-351)

ATPase family associated with various cellular activities (AAA) (AAA)

(115-246)

AAA domain (Cdc48 subfamily) (AAA_2)

(436-602)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  clpE Streptococcus mutans UA159

56.871

100

0.599
  clpE Streptococcus pneumoniae TIGR4

56.54

100

0.595
  clpE Streptococcus pneumoniae Rx1

56.54

100

0.595
  clpE Streptococcus pneumoniae D39

56.54

100

0.595
  clpE Streptococcus pneumoniae R6

56.54

100

0.595
  clpC Lactococcus lactis subsp. cremoris KW2

56.403

100

0.593
  clpC Bacillus subtilis subsp. subtilis str. 168

54.232

91.404

0.496
  clpC Lactococcus lactis subsp. lactis strain DGCC12653

46.154

91.261

0.421
  clpC Streptococcus thermophilus LMD-9

44.154

93.123

0.411
  clpC Streptococcus pneumoniae TIGR4

45.88

88.682

0.407
  clpC Streptococcus pneumoniae Rx1

45.88

88.682

0.407
  clpC Streptococcus pneumoniae D39

45.88

88.682

0.407
  clpC Streptococcus thermophilus LMG 18311

43.538

93.123

0.405
  clpC Streptococcus mutans UA159

44.246

88.395

0.391