Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   WA084_RS02160 Genome accession   NZ_CP146920
Coordinates   443930..444475 (+) Length   181 a.a.
NCBI ID   WP_014391406.1    Uniprot ID   -
Organism   Pasteurella multocida strain 212516     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 438930..449475
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WA084_RS02135 - 439357..440286 (+) 930 WP_014391409.1 phosphatidate cytidylyltransferase -
  WA084_RS02140 mog 440312..440890 (+) 579 WP_014391408.1 molybdopterin adenylyltransferase -
  WA084_RS02145 glnB 440935..441273 (+) 339 WP_005725112.1 nitrogen regulatory protein P-II -
  WA084_RS02150 - 441274..442527 (+) 1254 WP_338705836.1 AI-2E family transporter -
  WA084_RS02155 - 442707..443891 (+) 1185 WP_005752447.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  WA084_RS02160 pilF/pilF2 443930..444475 (+) 546 WP_014391406.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  WA084_RS02165 - 444598..445554 (+) 957 WP_014391405.1 RodZ family helix-turn-helix domain-containing protein -
  WA084_RS02170 ispG 445581..446684 (+) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  WA084_RS02175 hisS 446714..447985 (+) 1272 WP_041423113.1 histidine--tRNA ligase -
  WA084_RS02180 - 448004..448615 (+) 612 WP_005725137.1 YfgM family protein -
  WA084_RS02185 - 448824..449261 (+) 438 WP_005758199.1 isoprenylcysteine carboxylmethyltransferase family protein -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20884.46 Da        Isoelectric Point: 8.1419

>NTDB_id=945083 WA084_RS02160 WP_014391406.1 443930..444475(+) (pilF/pilF2) [Pasteurella multocida strain 212516]
MNRTFRFGIASIFSLLLCACVSQESNKTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGTFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFDPSRAKKFPPYIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=945083 WA084_RS02160 WP_014391406.1 443930..444475(+) (pilF/pilF2) [Pasteurella multocida strain 212516]
ATGAACCGCACTTTTCGATTTGGGATCGCGAGCATTTTTTCTCTTTTGCTTTGTGCTTGTGTGAGTCAAGAAAGTAACAA
AACGTTTGATTCACATGAGGCGGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCTGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGCGATGTATTAAA
TAATTATGGTACCTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTGGTTTGGTGTGCGTCTTTTGCCAAAGACGCTGTCCGTTATCAACAA
AATTTGCAACATTTAACGCAGTTTGATCCCAGTAGAGCAAAAAAATTTCCGCCATATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.695

92.265

0.486
  pilF Glaesserella parasuis strain SC1401

45.946

100

0.47