Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilH   Type   Machinery gene
Locus tag   ACFXAA_RS03050 Genome accession   NZ_CP170717
Coordinates   697963..698325 (-) Length   120 a.a.
NCBI ID   WP_003154238.1    Uniprot ID   A0A150FA43
Organism   Bacillus amyloliquefaciens strain JP5014     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 692963..703325
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFXAA_RS03025 (ACFXAA_03025) flhB 694488..695567 (-) 1080 WP_007409787.1 flagellar biosynthesis protein FlhB -
  ACFXAA_RS03030 (ACFXAA_03030) fliR 695567..696346 (-) 780 WP_003154233.1 flagellar biosynthetic protein FliR -
  ACFXAA_RS03035 (ACFXAA_03035) fliQ 696353..696622 (-) 270 WP_003154234.1 flagellar biosynthesis protein FliQ -
  ACFXAA_RS03040 (ACFXAA_03040) fliP 696637..697302 (-) 666 WP_007409786.1 flagellar type III secretion system pore protein FliP -
  ACFXAA_RS03045 (ACFXAA_03045) fliZ 697295..697948 (-) 654 WP_003154237.1 flagella biosynthesis regulatory protein FliZ -
  ACFXAA_RS03050 (ACFXAA_03050) pilH 697963..698325 (-) 363 WP_003154238.1 chemotaxis protein CheY Machinery gene
  ACFXAA_RS03055 (ACFXAA_03055) fliY 698352..699488 (-) 1137 WP_039251507.1 flagellar motor switch phosphatase FliY -
  ACFXAA_RS03060 (ACFXAA_03060) fliM 699478..700476 (-) 999 WP_003154240.1 flagellar motor switch protein FliM -
  ACFXAA_RS03065 (ACFXAA_03065) fliL 700510..700929 (-) 420 WP_014417773.1 flagellar basal body-associated protein FliL -
  ACFXAA_RS03070 (ACFXAA_03070) - 700926..701141 (-) 216 WP_003154243.1 flagellar FlbD family protein -
  ACFXAA_RS03075 (ACFXAA_03075) flgG 701181..701957 (-) 777 WP_015417471.1 flagellar basal body rod protein FlgG -
  ACFXAA_RS03080 (ACFXAA_03080) flgD 701982..702422 (-) 441 WP_015417470.1 flagellar hook assembly protein FlgD -

Sequence

Protein


Download         Length: 120 a.a.        Molecular weight: 13209.53 Da        Isoelectric Point: 4.6805

>NTDB_id=944985 ACFXAA_RS03050 WP_003154238.1 697963..698325(-) (pilH) [Bacillus amyloliquefaciens strain JP5014]
MAHRILIVDDAAFMRMMIKDILVKNGFDVVAEASDGAQAVEKFKEHSPDLVTMDITMPEMDGITALKEIKQIDPQAKIIM
CSAMGQQSMVIDAIQAGAKDFIVKPFQADRVLEAINKTLS

Nucleotide


Download         Length: 363 bp        

>NTDB_id=944985 ACFXAA_RS03050 WP_003154238.1 697963..698325(-) (pilH) [Bacillus amyloliquefaciens strain JP5014]
ATGGCACACAGAATTTTAATTGTAGACGATGCAGCATTTATGAGAATGATGATCAAAGATATTTTAGTGAAGAACGGTTT
TGACGTTGTAGCTGAAGCTTCAGACGGAGCGCAGGCAGTCGAAAAATTCAAAGAGCATTCCCCCGACCTTGTGACAATGG
ATATTACAATGCCTGAAATGGACGGGATTACAGCTTTAAAGGAAATTAAACAAATCGATCCGCAGGCGAAAATCATCATG
TGTTCGGCTATGGGCCAGCAGTCAATGGTTATTGACGCGATTCAAGCCGGCGCAAAAGACTTTATCGTGAAACCGTTCCA
AGCGGACCGCGTGCTTGAAGCTATTAATAAAACATTAAGCTAA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(5-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A150FA43

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilH Synechocystis sp. PCC 6803

38.983

98.333

0.383