Detailed information    

insolico Bioinformatically predicted

Overview

Name   sepM   Type   Regulator
Locus tag   ACFXAD_RS13100 Genome accession   NZ_CP170653
Coordinates   2676199..2677221 (-) Length   340 a.a.
NCBI ID   WP_012117493.1    Uniprot ID   A7Z4C8
Organism   Bacillus amyloliquefaciens strain JP5011     
Function   processing of CSP (predicted from homology)   
Competence regulation

Genomic Context

Location: 2671199..2682221
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFXAD_RS13070 (ACFXAD_13070) - 2671704..2672585 (-) 882 WP_053573414.1 2-dehydropantoate 2-reductase -
  ACFXAD_RS13075 (ACFXAD_13075) - 2672748..2673230 (+) 483 WP_007611262.1 N-acetyltransferase -
  ACFXAD_RS13080 (ACFXAD_13080) gerR 2673271..2673846 (-) 576 WP_014417712.1 sporulation-specific transcriptional regulator GerR -
  ACFXAD_RS13085 (ACFXAD_13085) rpmF 2674005..2674184 (-) 180 WP_003154457.1 50S ribosomal protein L32 -
  ACFXAD_RS13090 (ACFXAD_13090) - 2674206..2674724 (-) 519 WP_007611260.1 DUF177 domain-containing protein -
  ACFXAD_RS13095 (ACFXAD_13095) - 2674933..2676180 (+) 1248 WP_021493721.1 nucleotidyltransferase -
  ACFXAD_RS13100 (ACFXAD_13100) sepM 2676199..2677221 (-) 1023 WP_012117493.1 SepM family pheromone-processing serine protease Regulator
  ACFXAD_RS13105 (ACFXAD_13105) - 2677227..2678006 (-) 780 WP_014304961.1 patatin family protein -
  ACFXAD_RS13110 (ACFXAD_13110) ylbJ 2678186..2679412 (+) 1227 WP_053573413.1 sporulation integral membrane protein YlbJ -
  ACFXAD_RS13115 (ACFXAD_13115) coaD 2679431..2679913 (-) 483 WP_014417707.1 pantetheine-phosphate adenylyltransferase -
  ACFXAD_RS13120 (ACFXAD_13120) rsmD 2679918..2680472 (-) 555 WP_007409693.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  ACFXAD_RS13125 (ACFXAD_13125) - 2680771..2681043 (-) 273 WP_014417705.1 YlbG family protein -
  ACFXAD_RS13130 (ACFXAD_13130) ylbF 2681102..2681551 (-) 450 WP_003154472.1 YlbF family regulator Regulator
  ACFXAD_RS13135 (ACFXAD_13135) - 2681622..2681861 (-) 240 WP_003154475.1 YlbE-like family protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 37434.97 Da        Isoelectric Point: 7.3836

>NTDB_id=944917 ACFXAD_RS13100 WP_012117493.1 2676199..2677221(-) (sepM) [Bacillus amyloliquefaciens strain JP5011]
MKAKHFRWVFIILILLIAVTFIKLPYYITQPGEASELRPLIKVDGGYPEKGSLSLMTVKVGPANPYTYLWAKVHPYDEIV
PDESIKEEGESDSDYMKRQLQMMKSSQENAVIAAYQKAGKQVKYSFNGIYASSVVSDMPAKGKIEVGDKIISADGKTYES
AEKLIDYISSKKAGEKVTFHIERDTKEKTVDIVLKSFPDDPKRAGIGVALYTDRNVKVKPDLHFSIENIGGPSAGLMMSL
EIYNQLTKPDETKGYNIAGTGTIDVDGKVGPIGGIDQKVVAADKAGKDIFFAPNQNGAENSDYKNAVKTAKAIKSDMKIV
PVDTMQDALDYLDQLKVKST

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=944917 ACFXAD_RS13100 WP_012117493.1 2676199..2677221(-) (sepM) [Bacillus amyloliquefaciens strain JP5011]
ATGAAAGCAAAACATTTTCGCTGGGTGTTTATCATCCTGATCTTATTAATCGCTGTTACATTTATTAAGCTTCCGTATTA
CATCACACAGCCGGGAGAAGCATCAGAGCTGAGACCGCTGATTAAGGTGGACGGAGGCTATCCTGAAAAAGGAAGCCTTT
CCTTGATGACTGTCAAAGTAGGCCCTGCCAATCCGTATACATATCTTTGGGCGAAAGTGCATCCTTACGATGAAATCGTC
CCTGACGAAAGCATAAAAGAAGAGGGCGAATCGGACTCGGATTATATGAAGCGCCAGCTGCAAATGATGAAAAGCTCCCA
AGAAAACGCGGTCATTGCCGCTTATCAAAAAGCCGGGAAACAGGTGAAATACTCATTTAACGGCATTTATGCCAGCTCAG
TCGTCTCTGACATGCCGGCAAAAGGAAAAATAGAGGTCGGTGATAAAATCATCAGCGCGGACGGGAAAACATACGAATCC
GCTGAAAAGCTGATTGATTATATCAGCAGCAAAAAAGCGGGGGAAAAAGTAACGTTTCACATTGAACGGGACACGAAAGA
AAAGACCGTTGACATTGTGCTGAAATCATTCCCGGACGACCCGAAAAGAGCGGGAATCGGCGTCGCGTTATATACGGACC
GGAACGTGAAAGTGAAGCCGGATCTTCATTTCAGCATTGAAAACATCGGCGGGCCTTCTGCGGGTCTCATGATGTCTTTA
GAAATTTACAACCAGCTGACAAAACCTGACGAAACAAAAGGCTATAATATAGCCGGAACGGGGACAATCGACGTGGACGG
CAAAGTCGGACCGATCGGCGGAATCGATCAGAAAGTCGTCGCCGCAGACAAAGCGGGAAAAGATATTTTCTTTGCGCCGA
ATCAAAACGGAGCTGAAAATTCTGATTACAAAAATGCGGTAAAAACGGCGAAAGCAATCAAGTCCGATATGAAGATCGTC
CCTGTCGACACCATGCAGGACGCACTTGACTATTTGGATCAGCTGAAAGTAAAAAGCACCTGA

Domains

Predicted by InterProScan.

PDZ domain (PDZ_2)

(127-193)

Peptidase family M50 (Peptidase_M50)

(100-218)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(230-330)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7Z4C8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sepM Streptococcus mutans UA159

38.953

100

0.394