Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilH   Type   Machinery gene
Locus tag   ACFXAD_RS12470 Genome accession   NZ_CP170653
Coordinates   2547089..2547451 (-) Length   120 a.a.
NCBI ID   WP_003154238.1    Uniprot ID   A0A150FA43
Organism   Bacillus amyloliquefaciens strain JP5011     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2542089..2552451
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFXAD_RS12445 (ACFXAD_12445) flhB 2543614..2544693 (-) 1080 WP_007409787.1 flagellar biosynthesis protein FlhB -
  ACFXAD_RS12450 (ACFXAD_12450) fliR 2544693..2545472 (-) 780 WP_017417780.1 flagellar biosynthetic protein FliR -
  ACFXAD_RS12455 (ACFXAD_12455) fliQ 2545479..2545748 (-) 270 WP_003154234.1 flagellar biosynthesis protein FliQ -
  ACFXAD_RS12460 (ACFXAD_12460) fliP 2545763..2546428 (-) 666 WP_007409786.1 flagellar type III secretion system pore protein FliP -
  ACFXAD_RS12465 (ACFXAD_12465) fliZ 2546421..2547074 (-) 654 WP_003154237.1 flagella biosynthesis regulatory protein FliZ -
  ACFXAD_RS12470 (ACFXAD_12470) pilH 2547089..2547451 (-) 363 WP_003154238.1 chemotaxis protein CheY Machinery gene
  ACFXAD_RS12475 (ACFXAD_12475) fliY 2547478..2548614 (-) 1137 WP_015417472.1 flagellar motor switch phosphatase FliY -
  ACFXAD_RS12480 (ACFXAD_12480) fliM 2548604..2549602 (-) 999 WP_003154240.1 flagellar motor switch protein FliM -
  ACFXAD_RS12485 (ACFXAD_12485) fliL 2549636..2550055 (-) 420 WP_014417773.1 flagellar basal body-associated protein FliL -
  ACFXAD_RS12490 (ACFXAD_12490) - 2550052..2550267 (-) 216 WP_003154243.1 flagellar FlbD family protein -
  ACFXAD_RS12495 (ACFXAD_12495) flgG 2550307..2551083 (-) 777 WP_007409783.1 flagellar basal body rod protein FlgG -
  ACFXAD_RS12500 (ACFXAD_12500) flgD 2551108..2551548 (-) 441 WP_015239855.1 flagellar hook assembly protein FlgD -

Sequence

Protein


Download         Length: 120 a.a.        Molecular weight: 13209.53 Da        Isoelectric Point: 4.6805

>NTDB_id=944909 ACFXAD_RS12470 WP_003154238.1 2547089..2547451(-) (pilH) [Bacillus amyloliquefaciens strain JP5011]
MAHRILIVDDAAFMRMMIKDILVKNGFDVVAEASDGAQAVEKFKEHSPDLVTMDITMPEMDGITALKEIKQIDPQAKIIM
CSAMGQQSMVIDAIQAGAKDFIVKPFQADRVLEAINKTLS

Nucleotide


Download         Length: 363 bp        

>NTDB_id=944909 ACFXAD_RS12470 WP_003154238.1 2547089..2547451(-) (pilH) [Bacillus amyloliquefaciens strain JP5011]
ATGGCACACAGAATTTTAATTGTAGACGATGCAGCATTTATGAGAATGATGATCAAAGATATTTTAGTGAAGAACGGTTT
TGACGTTGTAGCTGAAGCTTCAGACGGAGCGCAGGCAGTCGAAAAATTCAAAGAGCATTCCCCCGACCTTGTGACAATGG
ATATTACAATGCCTGAAATGGACGGGATTACAGCTTTAAAGGAAATTAAACAAATCGATCCGCAGGCGAAAATCATCATG
TGTTCGGCTATGGGCCAGCAGTCAATGGTTATTGACGCGATTCAAGCCGGCGCAAAAGACTTTATCGTGAAACCGTTCCA
AGCGGACCGCGTGCTTGAAGCTATTAATAAAACATTAAGCTAA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(5-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A150FA43

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilH Synechocystis sp. PCC 6803

38.983

98.333

0.383