Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACFW99_RS16695 Genome accession   NZ_CP170651
Coordinates   3383218..3384528 (-) Length   436 a.a.
NCBI ID   WP_061890959.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain JP5025     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3378218..3389528
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFW99_RS16675 (ACFW99_16675) - 3378461..3379177 (-) 717 WP_015418259.1 (Fe-S)-binding protein -
  ACFW99_RS16680 (ACFW99_16680) - 3379358..3380548 (-) 1191 WP_156240486.1 PLP-dependent aminotransferase family protein -
  ACFW99_RS16685 (ACFW99_16685) - 3380579..3381298 (-) 720 WP_017418877.1 FadR/GntR family transcriptional regulator -
  ACFW99_RS16690 (ACFW99_16690) lutP 3381504..3383180 (+) 1677 WP_031378309.1 L-lactate permease LutP -
  ACFW99_RS16695 (ACFW99_16695) rpoN/rpoN1 3383218..3384528 (-) 1311 WP_061890959.1 RNA polymerase factor sigma-54 Machinery gene
  ACFW99_RS16700 (ACFW99_16700) yvfG 3384606..3384824 (+) 219 WP_003151570.1 protein YvfG -
  ACFW99_RS16705 (ACFW99_16705) - 3384838..3385803 (-) 966 WP_039253776.1 polysaccharide pyruvyl transferase family protein -
  ACFW99_RS16710 (ACFW99_16710) - 3385782..3386954 (-) 1173 WP_007407381.1 DegT/DnrJ/EryC1/StrS aminotransferase family protein -
  ACFW99_RS16715 (ACFW99_16715) - 3386959..3387606 (-) 648 WP_031378307.1 acetyltransferase -
  ACFW99_RS16720 (ACFW99_16720) - 3387603..3388211 (-) 609 WP_007407383.1 sugar transferase -

Sequence

Protein


Download         Length: 436 a.a.        Molecular weight: 49805.70 Da        Isoelectric Point: 9.2644

>NTDB_id=944795 ACFW99_RS16695 WP_061890959.1 3383218..3384528(-) (rpoN/rpoN1) [Bacillus amyloliquefaciens strain JP5025]
MDIKLQQTQMLKPQLTQELKQAITLLGYNSAELAGYIDELSLENPLIERKETDTPPLSYHKTSKNRMKERDGSQQIRHTR
KTLQDSLKQQALDMKLSDDEKKIFNYLIHSLDSNGYLKEDIQAAADHVSASQEETEAVLKKLQSLEPAGIGARSLQECIL
LQLRRLPERNEQAEWIAGAYFELFARKNWKALTLKTGISLKDIQDIADMISALDPRPGLRYAHEEPGFYVEPDIFITVKN
GIIQAQLNSRSFPDIQLHTHYQPLLSAAGCRDTKNYLSAKYQEWRWLNRALSQRKQTITRIIGVLTERQESFFLTGKSGM
QPLTLREVAETLELHESTISRAIKGKYVQTPYGLYEMKTFFSAKAESSGDGGASNYAVKAHLEALVGKEDKKKPFSDQKL
ADLLHEQYDIRISRRTVAKYRDQLNIPPSSARKRYS

Nucleotide


Download         Length: 1311 bp        

>NTDB_id=944795 ACFW99_RS16695 WP_061890959.1 3383218..3384528(-) (rpoN/rpoN1) [Bacillus amyloliquefaciens strain JP5025]
ATGGATATCAAACTTCAGCAAACACAAATGTTAAAGCCTCAATTAACGCAAGAGCTTAAACAGGCTATCACATTACTCGG
ATACAATTCCGCGGAACTGGCCGGATATATTGATGAGCTCTCACTGGAAAACCCTCTGATTGAACGAAAGGAAACAGACA
CGCCGCCACTATCTTACCATAAAACAAGTAAAAACAGGATGAAAGAACGGGACGGAAGCCAGCAGATCAGACATACCCGC
AAAACGCTGCAGGATTCGCTCAAACAGCAAGCCTTAGATATGAAGCTGTCCGATGATGAGAAAAAGATTTTCAATTATCT
GATACATTCATTGGACTCAAACGGATATTTAAAAGAGGACATACAAGCTGCCGCCGACCATGTATCCGCTTCACAAGAGG
AGACGGAAGCGGTTCTGAAAAAGCTCCAGTCCCTTGAGCCCGCGGGAATCGGCGCCCGGTCTTTGCAGGAATGCATCCTT
CTTCAGCTCCGCCGTCTTCCCGAGCGGAACGAACAGGCGGAATGGATTGCCGGCGCCTATTTTGAGCTATTTGCAAGAAA
GAACTGGAAAGCGCTTACACTAAAAACGGGCATTTCGCTTAAAGACATTCAGGACATCGCTGACATGATCAGCGCCCTTG
ATCCGAGGCCGGGGCTCCGTTATGCGCATGAGGAGCCGGGTTTTTACGTGGAACCGGATATTTTCATCACAGTGAAAAAC
GGCATCATTCAAGCGCAGCTCAACAGCCGTTCGTTTCCTGACATCCAGCTTCATACCCATTATCAGCCGCTCTTGTCAGC
TGCCGGGTGCCGTGACACGAAAAATTATCTGTCGGCAAAATATCAGGAGTGGCGCTGGCTGAACCGGGCTTTGTCGCAAA
GAAAACAAACGATCACGAGAATTATCGGCGTCCTGACTGAACGGCAGGAATCCTTTTTTCTCACAGGAAAAAGCGGAATG
CAGCCGCTGACCTTAAGAGAGGTGGCGGAAACGCTGGAGCTCCACGAATCAACGATCAGCCGGGCCATCAAAGGGAAGTA
CGTTCAGACGCCGTACGGACTGTATGAAATGAAAACATTTTTCTCGGCGAAAGCTGAATCGTCGGGGGACGGCGGCGCCT
CCAATTATGCCGTAAAAGCGCATCTGGAGGCATTGGTCGGTAAAGAAGATAAAAAGAAACCTTTTTCAGATCAAAAGCTT
GCCGATCTGCTGCATGAACAATATGACATCCGAATCTCAAGGCGCACCGTCGCCAAATACCGTGACCAATTAAATATTCC
GCCGTCATCGGCGAGAAAACGGTACAGCTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(75-261)

Sigma-54, DNA binding domain (Sigma54_DBD)

(275-434)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

33.467

100

0.383