Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilH   Type   Machinery gene
Locus tag   ACFW99_RS08425 Genome accession   NZ_CP170651
Coordinates   1674767..1675129 (+) Length   120 a.a.
NCBI ID   WP_003154238.1    Uniprot ID   A0A150FA43
Organism   Bacillus amyloliquefaciens strain JP5025     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 1669767..1680129
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFW99_RS08395 (ACFW99_08395) flgD 1670670..1671110 (+) 441 WP_015417470.1 flagellar hook assembly protein FlgD -
  ACFW99_RS08400 (ACFW99_08400) flgG 1671135..1671911 (+) 777 WP_015417471.1 flagellar basal body rod protein FlgG -
  ACFW99_RS08405 (ACFW99_08405) - 1671951..1672166 (+) 216 WP_003154243.1 flagellar FlbD family protein -
  ACFW99_RS08410 (ACFW99_08410) fliL 1672163..1672582 (+) 420 WP_014417773.1 flagellar basal body-associated protein FliL -
  ACFW99_RS08415 (ACFW99_08415) fliM 1672616..1673614 (+) 999 WP_003154240.1 flagellar motor switch protein FliM -
  ACFW99_RS08420 (ACFW99_08420) fliY 1673604..1674740 (+) 1137 WP_039251507.1 flagellar motor switch phosphatase FliY -
  ACFW99_RS08425 (ACFW99_08425) pilH 1674767..1675129 (+) 363 WP_003154238.1 chemotaxis protein CheY Machinery gene
  ACFW99_RS08430 (ACFW99_08430) fliZ 1675144..1675797 (+) 654 WP_003154237.1 flagella biosynthesis regulatory protein FliZ -
  ACFW99_RS08435 (ACFW99_08435) fliP 1675790..1676455 (+) 666 WP_007409786.1 flagellar type III secretion system pore protein FliP -
  ACFW99_RS08440 (ACFW99_08440) fliQ 1676470..1676739 (+) 270 WP_003154234.1 flagellar biosynthesis protein FliQ -
  ACFW99_RS08445 (ACFW99_08445) fliR 1676746..1677525 (+) 780 WP_003154233.1 flagellar biosynthetic protein FliR -
  ACFW99_RS08450 (ACFW99_08450) flhB 1677525..1678604 (+) 1080 WP_007409787.1 flagellar biosynthesis protein FlhB -

Sequence


Protein


Download         Length: 120 a.a.        Molecular weight: 13209.53 Da        Isoelectric Point: 4.6805

>NTDB_id=944736 ACFW99_RS08425 WP_003154238.1 1674767..1675129(+) (pilH) [Bacillus amyloliquefaciens strain JP5025]
MAHRILIVDDAAFMRMMIKDILVKNGFDVVAEASDGAQAVEKFKEHSPDLVTMDITMPEMDGITALKEIKQIDPQAKIIM
CSAMGQQSMVIDAIQAGAKDFIVKPFQADRVLEAINKTLS

Nucleotide


Download         Length: 363 bp        

>NTDB_id=944736 ACFW99_RS08425 WP_003154238.1 1674767..1675129(+) (pilH) [Bacillus amyloliquefaciens strain JP5025]
ATGGCACACAGAATTTTAATTGTAGACGATGCAGCATTTATGAGAATGATGATCAAAGATATTTTAGTGAAGAACGGTTT
TGACGTTGTAGCTGAAGCTTCAGACGGAGCGCAGGCAGTCGAAAAATTCAAAGAGCATTCCCCCGACCTTGTGACAATGG
ATATTACAATGCCTGAAATGGACGGGATTACAGCTTTAAAGGAAATTAAACAAATCGATCCGCAGGCGAAAATCATCATG
TGTTCGGCTATGGGCCAGCAGTCAATGGTTATTGACGCGATTCAAGCCGGCGCAAAAGACTTTATCGTGAAACCGTTCCA
AGCGGACCGCGTGCTTGAAGCTATTAATAAAACATTAAGCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A150FA43

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilH Synechocystis sp. PCC 6803

38.983

98.333

0.383