Detailed information    

insolico Bioinformatically predicted

Overview

Name   clpE   Type   Regulator
Locus tag   ACFW99_RS07045 Genome accession   NZ_CP170651
Coordinates   1358304..1360400 (-) Length   698 a.a.
NCBI ID   WP_317859653.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain JP5025     
Function   repress competence development (at the early growth phase) (predicted from homology)   
Competence regulation

Genomic Context

Location: 1353304..1365400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFW99_RS07020 (ACFW99_07020) - 1353893..1354153 (+) 261 WP_003154690.1 aspartyl-phosphate phosphatase Spo0E family protein -
  ACFW99_RS07025 (ACFW99_07025) - 1354363..1355880 (-) 1518 WP_046559543.1 ATP-binding protein -
  ACFW99_RS07030 (ACFW99_07030) - 1356079..1356525 (+) 447 WP_015417342.1 MarR family winged helix-turn-helix transcriptional regulator -
  ACFW99_RS07035 (ACFW99_07035) motB 1356562..1357347 (-) 786 WP_003154685.1 flagellar motor protein MotB -
  ACFW99_RS07040 (ACFW99_07040) motA 1357319..1358134 (-) 816 WP_007408461.1 flagellar motor stator protein MotA -
  ACFW99_RS07045 (ACFW99_07045) clpE 1358304..1360400 (-) 2097 WP_317859653.1 ATP-dependent protease ATP-binding subunit ClpE Regulator
  ACFW99_RS07050 (ACFW99_07050) - 1360802..1361839 (+) 1038 WP_020953988.1 transporter -
  ACFW99_RS07055 (ACFW99_07055) - 1361937..1363058 (+) 1122 WP_161619881.1 hypothetical protein -
  ACFW99_RS07060 (ACFW99_07060) queC 1363308..1363967 (+) 660 WP_003154679.1 7-cyano-7-deazaguanine synthase QueC -
  ACFW99_RS07065 (ACFW99_07065) queD 1363968..1364411 (+) 444 WP_007408455.1 6-carboxytetrahydropterin synthase QueD -
  ACFW99_RS07070 (ACFW99_07070) queE 1364404..1365135 (+) 732 WP_015239741.1 7-carboxy-7-deazaguanine synthase QueE -

Sequence

Protein


Download         Length: 698 a.a.        Molecular weight: 77798.86 Da        Isoelectric Point: 5.4325

>NTDB_id=944722 ACFW99_RS07045 WP_317859653.1 1358304..1360400(-) (clpE) [Bacillus amyloliquefaciens strain JP5025]
MRCQHCQKNEATIRLNMQVNSIQKQFVLCETCYTELTRKPSMSMGPQSFGFPFEQGFAPNQNKQQAGPEKGLLDELAQNV
TNGAKAGLIDPVIGRDHEVARVIEILNRRNKNNPVLIGEPGVGKTAIAEGLALKIAEGDVPNKLKNKELFLLDVASLVAN
TGIRGQFEERMKQLMNELKERKNVILFIDEIHLLVGTGSAEGSMDAGNILKPALARGELQVIGATTLKEYRQIEKDAALE
RRFQPVMVHEPTIEQAISILEGIKDKYEKYHGVTFSNEAINACVTLSSRYIQDRHLPDKAIDLLDEAGSKANLELDTVNE
EDAAERLKAIDAEKTKALHEENYELAAKLRDEEAALEKQLAHETPASSPEIGAEHIQAIIEQKTGIPVGKLQADEQVKMK
ELEEHLHQRVIGQEKAVKKVAKAVRRSRAGLKSKNRPVGSFLFVGPTGVGKTELSKTLADELFGTKDSIIRLDMSEYMEK
HAVSKIIGSPPGYVGHDEAGQLTEKVRRNPYSIVLLDEIEKAHPDVQHMFLQIMEDGRLTDSQGRTVSFKDTVLIMTSNA
GTGEKQVKVGFQSEESVLEEQTLIDSLSAFFKPEFLNRFDSIIEFQSLKKEHLVKIVSLLLTELEDTLKERGISLSVTDE
AKEKIAEIGYHPAFGARPLRRTIQELIEDEMTDLLLDNNEIKAFHVVMEEDGIKVRAQ

Nucleotide


Download         Length: 2097 bp        

>NTDB_id=944722 ACFW99_RS07045 WP_317859653.1 1358304..1360400(-) (clpE) [Bacillus amyloliquefaciens strain JP5025]
ATGCGTTGTCAACATTGTCAGAAAAACGAGGCGACGATTCGCCTGAACATGCAAGTCAATTCCATTCAAAAACAATTCGT
TTTATGTGAAACATGTTATACAGAATTAACCCGAAAACCGTCTATGAGCATGGGGCCGCAATCATTCGGATTCCCGTTTG
AGCAAGGTTTCGCACCCAATCAAAATAAACAGCAGGCCGGCCCTGAAAAAGGCCTGCTCGACGAGCTCGCTCAAAACGTC
ACAAACGGCGCAAAAGCAGGTCTGATTGATCCTGTGATCGGCCGCGATCATGAAGTGGCGCGCGTAATTGAAATTTTGAA
CCGCCGGAATAAAAACAATCCGGTGCTGATCGGTGAACCGGGCGTCGGTAAAACGGCTATTGCAGAAGGCCTCGCGCTTA
AAATCGCTGAAGGCGATGTGCCGAACAAATTGAAAAACAAAGAGCTTTTCCTGCTTGACGTCGCTTCTCTCGTCGCCAAT
ACAGGGATCAGAGGCCAGTTTGAAGAAAGAATGAAACAATTAATGAACGAATTAAAAGAACGGAAAAACGTCATCTTGTT
CATTGACGAGATTCACCTTCTTGTAGGCACCGGCTCTGCCGAGGGATCAATGGATGCCGGGAACATCCTGAAACCGGCGC
TTGCCAGAGGCGAGCTGCAGGTCATCGGGGCGACAACACTGAAAGAGTACCGCCAGATTGAAAAAGATGCGGCGCTTGAA
AGACGCTTCCAGCCTGTCATGGTTCACGAGCCTACAATAGAACAAGCCATCAGCATTCTGGAAGGCATTAAAGATAAATA
TGAGAAATACCACGGCGTCACATTCAGCAATGAAGCGATAAACGCCTGTGTTACGCTTTCATCCCGTTACATTCAGGACC
GCCACCTGCCGGATAAAGCGATCGACTTATTAGATGAAGCCGGCTCTAAGGCGAACCTGGAGCTTGATACCGTAAATGAA
GAAGATGCGGCTGAGAGACTCAAAGCAATTGACGCGGAGAAAACAAAAGCGCTTCATGAAGAAAATTATGAGCTTGCCGC
GAAACTGCGTGATGAAGAAGCGGCGTTGGAAAAGCAGCTGGCGCATGAAACACCGGCCTCTTCACCTGAAATCGGGGCGG
AGCATATTCAAGCCATTATTGAACAGAAAACAGGCATTCCGGTCGGAAAACTCCAGGCTGATGAACAAGTTAAAATGAAG
GAACTTGAAGAGCATCTTCATCAGCGCGTCATCGGTCAAGAAAAAGCCGTGAAAAAAGTGGCGAAAGCGGTCAGACGAAG
CCGCGCCGGCTTAAAATCCAAAAACAGACCTGTCGGCTCCTTCCTCTTTGTCGGACCGACCGGAGTCGGAAAAACCGAGT
TATCAAAGACACTCGCAGATGAACTGTTCGGTACAAAAGATTCCATTATCCGTCTCGATATGAGTGAATACATGGAAAAA
CACGCCGTATCAAAAATCATCGGTTCACCGCCGGGATATGTCGGACACGATGAAGCCGGTCAATTAACGGAAAAAGTGCG
CCGCAATCCGTACAGCATCGTGCTGCTCGATGAGATTGAAAAAGCCCACCCTGATGTCCAGCATATGTTTCTGCAGATTA
TGGAGGACGGCCGTCTGACAGACAGCCAGGGCAGAACGGTCAGCTTCAAAGATACCGTTTTGATTATGACAAGCAACGCT
GGAACCGGCGAAAAACAGGTGAAAGTCGGATTCCAATCTGAAGAGAGCGTCCTGGAAGAACAAACGCTGATTGATTCACT
GAGCGCGTTCTTCAAACCGGAGTTTTTAAACCGGTTTGACAGCATCATTGAATTCCAATCCCTGAAAAAAGAACATCTCG
TGAAAATCGTCAGCCTGCTTCTCACAGAGCTTGAAGACACATTAAAAGAGCGCGGCATCAGCCTGAGCGTAACGGACGAA
GCGAAAGAAAAAATCGCTGAAATCGGCTACCACCCAGCTTTCGGAGCACGCCCGCTCAGAAGAACCATCCAAGAATTGAT
CGAGGACGAAATGACAGATCTCTTATTGGATAACAACGAAATAAAAGCCTTCCATGTCGTAATGGAAGAGGACGGCATCA
AAGTCCGGGCACAATAA

Domains

Predicted by InterProScan.

C-terminal, D2-small domain, of ClpB protein (ClpB_D2-small)

(608-679)

AAA lid domain (AAA_lid_9)

(252-351)

ATPase family associated with various cellular activities (AAA) (AAA)

(115-246)

AAA domain (Cdc48 subfamily) (AAA_2)

(436-602)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  clpE Streptococcus mutans UA159

56.735

100

0.597
  clpC Lactococcus lactis subsp. cremoris KW2

56.54

100

0.595
  clpE Streptococcus pneumoniae Rx1

56.131

100

0.59
  clpE Streptococcus pneumoniae R6

56.131

100

0.59
  clpE Streptococcus pneumoniae D39

56.131

100

0.59
  clpE Streptococcus pneumoniae TIGR4

56.131

100

0.59
  clpC Bacillus subtilis subsp. subtilis str. 168

54.075

91.404

0.494
  clpC Lactococcus lactis subsp. lactis strain DGCC12653

46.154

91.261

0.421
  clpC Streptococcus thermophilus LMD-9

44.154

93.123

0.411
  clpC Streptococcus pneumoniae TIGR4

45.88

88.682

0.407
  clpC Streptococcus pneumoniae Rx1

45.88

88.682

0.407
  clpC Streptococcus pneumoniae D39

45.88

88.682

0.407
  clpC Streptococcus thermophilus LMG 18311

43.538

93.123

0.405
  clpC Streptococcus mutans UA159

44.3

87.966

0.39