Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   V9W62_RS00585 Genome accession   NZ_CP146763
Coordinates   106708..108087 (+) Length   459 a.a.
NCBI ID   WP_043021047.1    Uniprot ID   -
Organism   Bacillus velezensis strain AP81     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 101708..113087
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V9W62_RS00565 (V9W62_00565) ctsR 102056..102520 (+) 465 WP_003156396.1 transcriptional regulator CtsR -
  V9W62_RS00570 (V9W62_00570) - 102534..103091 (+) 558 WP_007410387.1 UvrB/UvrC motif-containing protein -
  V9W62_RS00575 (V9W62_00575) - 103091..104182 (+) 1092 WP_003156398.1 protein arginine kinase -
  V9W62_RS00580 (V9W62_00580) clpC 104179..106614 (+) 2436 WP_043021048.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  V9W62_RS00585 (V9W62_00585) radA 106708..108087 (+) 1380 WP_043021047.1 DNA repair protein RadA Machinery gene
  V9W62_RS00590 (V9W62_00590) disA 108091..109173 (+) 1083 WP_007615225.1 DNA integrity scanning diadenylate cyclase DisA -
  V9W62_RS00595 (V9W62_00595) - 109289..110389 (+) 1101 WP_003156403.1 PIN/TRAM domain-containing protein -
  V9W62_RS00600 (V9W62_00600) ispD 110402..111100 (+) 699 WP_015239038.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  V9W62_RS00605 (V9W62_00605) ispF 111093..111569 (+) 477 WP_003156407.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49506.91 Da        Isoelectric Point: 7.1316

>NTDB_id=944473 V9W62_RS00585 WP_043021047.1 106708..108087(+) (radA) [Bacillus velezensis strain AP81]
MAKSKTKFICHSCGYESAKWMGKCPGCGAWNTMVEETIKKAPANRRAAFSHSVQTVQKPSPISSIETSEEPRVQTKLEEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLADTAGSVLYISGEESVKQTKLRADRLGINSQILHVLSETDMEYIS
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGASGSSIVASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPEANADGWTIPKGIEVVGVANVAEALRTSLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=944473 V9W62_RS00585 WP_043021047.1 106708..108087(+) (radA) [Bacillus velezensis strain AP81]
ATGGCTAAATCAAAAACAAAATTCATCTGCCACTCATGCGGATATGAATCCGCAAAATGGATGGGAAAGTGCCCGGGCTG
CGGGGCTTGGAATACAATGGTGGAAGAAACCATAAAAAAAGCGCCGGCGAATCGGCGGGCGGCTTTCTCCCACTCCGTTC
AAACCGTCCAGAAACCATCACCCATTTCATCAATTGAAACATCGGAAGAACCCCGTGTTCAAACAAAGCTCGAAGAATTC
AACAGAGTGCTTGGCGGCGGCGTTGTGAAGGGCTCGTTAGTATTAATCGGCGGCGATCCCGGGATCGGAAAATCAACGCT
CCTTCTCCAAGTATCCGCACAATTAGCAGATACGGCCGGCAGCGTGCTTTACATTTCAGGGGAAGAATCCGTGAAGCAAA
CGAAGCTGCGCGCCGACAGACTTGGAATCAACAGCCAAATATTACATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAAACCCGCATTTGTTGTCGTAGACTCCATTCAGACCGTTTACCAAAGTGATATCACCTCGGC
TCCCGGCAGTGTATCACAAGTAAGAGAATGTACGGCTGAATTGATGAAAATCGCGAAAACAAACGGCATACCTATTTTTA
TCGTCGGGCACGTCACAAAAGAAGGATCGATCGCAGGGCCGAGATTGCTTGAGCACATGGTCGATACCGTTCTATACTTT
GAAGGAGAGCGGCACCATACATTCCGTATTTTGCGGGCCGTAAAAAACCGTTTCGGCTCAACAAATGAAATGGGCATCTT
TGAAATGCGGGAAGAGGGGCTTACGGAAGTGCTGAACCCTTCTGAAATTTTCTTGGAAGAGCGTTCTGCCGGGGCATCGG
GTTCGAGTATCGTTGCGTCAATGGAAGGAACGAGACCGATCCTGGTTGAAATTCAGGCGCTCATATCACCGACTAGTTTT
GGAAACCCGCGCCGCATGGCTACAGGCATTGACCACAACAGGGTGTCGTTAATTATGGCTGTGCTTGAGAAGCGGGTGGG
TCTTTTGCTTCAAAATCAAGATGCATATTTAAAGGTCGCCGGCGGGGTCAAACTGGATGAACCGGCGATTGATTTGGCAG
TAGCCGTCAGCATTGCCTCAAGCTTCAGAGATACACCGCCGAATCCCGCAGATTGCTTTATCGGTGAAGTGGGTCTGACA
GGAGAAGTCCGCAGGGTGTCAAGAATCGAACAGCGCGTAAAGGAAGCGGCAAAACTCGGCTTCAAGCGCATGATCATACC
TGAGGCGAATGCAGACGGGTGGACAATACCAAAAGGGATTGAGGTTGTCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATCATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(67-222)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

93.231

99.782

0.93
  radA Streptococcus pneumoniae Rx1

63.576

98.693

0.627
  radA Streptococcus pneumoniae D39

63.576

98.693

0.627
  radA Streptococcus pneumoniae R6

63.576

98.693

0.627
  radA Streptococcus pneumoniae TIGR4

63.576

98.693

0.627
  radA Streptococcus mitis NCTC 12261

63.576

98.693

0.627
  radA Streptococcus mitis SK321

63.355

98.693

0.625