Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   ACFIOK_RS05945 Genome accession   NZ_CP170434
Coordinates   1138699..1140972 (+) Length   757 a.a.
NCBI ID   WP_000594985.1    Uniprot ID   -
Organism   Staphylococcus aureus strain t315     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1133699..1145972
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFIOK_RS05930 tgt 1134524..1135663 (+) 1140 WP_001112045.1 tRNA guanosine(34) transglycosylase Tgt -
  ACFIOK_RS05935 yajC 1135682..1135942 (+) 261 WP_001160682.1 preprotein translocase subunit YajC -
  ACFIOK_RS05940 secDF 1136217..1138496 (+) 2280 WP_000749795.1 protein translocase subunit SecDF -
  ACFIOK_RS05945 recJ 1138699..1140972 (+) 2274 WP_000594985.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  ACFIOK_RS05950 - 1140994..1141512 (+) 519 WP_000364542.1 adenine phosphoribosyltransferase -
  ACFIOK_RS05955 - 1141939..1144128 (+) 2190 WP_001058583.1 RelA/SpoT family protein -
  ACFIOK_RS05960 dtd 1144140..1144592 (+) 453 WP_000869983.1 D-aminoacyl-tRNA deacylase -
  ACFIOK_RS05965 - 1144589..1145464 (+) 876 WP_000717800.1 N-acetylmuramoyl-L-alanine amidase -

Sequence

Protein


Download         Length: 757 a.a.        Molecular weight: 85634.84 Da        Isoelectric Point: 4.8732

>NTDB_id=944312 ACFIOK_RS05945 WP_000594985.1 1138699..1140972(+) (recJ) [Staphylococcus aureus strain t315]
MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQAIESIISDTDFNHDALQLSDMTKAIERIKRAIANDE
KILVYGDYDADGVTSTTILVTTLQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
VDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFKALVAIGTIADLVSLTDENRSLV
KQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFIIGPRLNAVGRLDDASLACELLMTDDEEEAAFLAEQVEHFNRERK
DIVATITEEAMAMAETKVKKGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSIDQVSMFEIL
SAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVKQVDVLLTENDITIKNIRDMNRLRPFGTDFS
RPIFEMDDLSVSSVKAIGQQKNHLKLTLGESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQIIIQDIAMN
EQQILDYRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKQQTDKVVLRDLPTSMEDLSNSLQQLQFSQLYIVL
QHNHSIYFDGIPNMDIFKKCYKALITKQETNIQKEGMLLCQHLSVKPDTLKFMLKVFLDLKFVTQEDGLIRINQQPDKRS
IDSSKVYQLRQQRMDVEKQLLYQDFSEIKNWIKSQLS

Nucleotide


Download         Length: 2274 bp        

>NTDB_id=944312 ACFIOK_RS05945 WP_000594985.1 1138699..1140972(+) (recJ) [Staphylococcus aureus strain t315]
ATGATTAAACCGAAGTATAAGTGGAAATTAACTAAACCAGCTGAATACATAAGTGATGAATTAACATCAAAATTAAAGTT
AACGCCAATCGTAAAAAAAATATTAGAAAGTAAATCGATTATTGATGAACAAGCAATTGAATCCATCATTTCAGACACAG
ATTTCAATCATGATGCATTGCAATTGAGCGATATGACTAAGGCTATTGAAAGAATTAAAAGAGCGATAGCGAATGATGAG
AAAATCCTAGTTTATGGTGATTACGATGCCGACGGTGTTACCTCAACTACAATTTTAGTGACAACATTACAACTTTTAGG
TGCACAAGTAGGATGGCATATTCCTAACCGCTTTACCGAAGGATATGGACCAAACGAACTAGCATTTAGAAATGCTCATG
ATGAAGGCATAACATTAATCATAACTGTTGATAATGGCATACAAGGTCATAATGAAATTAAAATGGTTCAAGATTTAGGA
GTTGATGTCATTGTAACTGATCATCATGAAATTGGTAGTACATTACCAGAAGCATATGCAATCGTACATCCAATGCATCC
ATCGTTTAATTATCCGTTCCAACAATTGTGTGGTGCAGGTGTTGCATACAAATTAGCACAAGCATTGATTGAAAATGTAC
CTGATTATTTTAAAGCATTAGTTGCGATAGGTACCATTGCGGATTTAGTATCCTTAACTGATGAAAATCGATCATTAGTG
AAACAAGGATTAAAAGTCTTAAATGATCAATGTCCGACCTCTGTTAAAGCATTGCTAAAAGAAGCGGGATACAATGACAA
TATTGATGAAGAAACAATTGGTTTTATTATTGGACCTAGACTTAATGCTGTAGGTCGTTTAGATGACGCATCATTAGCTT
GCGAATTGTTGATGACTGACGACGAAGAAGAAGCTGCATTTTTAGCAGAACAAGTTGAACATTTTAACCGTGAAAGAAAA
GATATTGTAGCAACTATCACAGAAGAAGCAATGGCTATGGCAGAAACGAAAGTTAAAAAGGGAGATTTATTTTTACTTTT
AGCAAAAGAAAATTGGCATGAAGGTGTCTTAGGTATTGTTGCTTCGAAAATTGTCGAAACATTTGCTTTGCCAACATTAA
TTTTAAATATTGATAGGGAGCAAAATCATGCAAAAGGTTCCGCCAGATCAATTGATCAAGTGTCGATGTTTGAAATACTA
TCAGCACATCAAGAGTTAATCGCTAAATTTGGCGGCCATCATATGGCTGCAGGTATGACGATGGATATTGAAAATATAGA
ATCGTTAGCTGAAGGTTTAAATAAATGGATGAAAGAATTATCTAAAACAACTTCGTTAGATCCCGTAAAGCAAGTAGATG
TATTATTAACAGAAAATGATATAACGATTAAAAATATTCGTGATATGAATCGTTTAAGACCCTTCGGAACAGATTTTTCT
AGACCTATTTTTGAAATGGATGATTTATCAGTTTCTTCAGTTAAGGCGATAGGTCAACAAAAAAATCATCTAAAATTGAC
ACTTGGTGAAAGTAATATCGCCGCGCTATTTTGGCAAAATGGACACTTAGAACCTGAGTTACAAGATGAACAGCCAATTA
ATATATTAGGATCTGTTCAAATCAACGAATGGAATGGTAATCAATCACCGCAAATAATTATTCAAGATATTGCGATGAAT
GAACAGCAAATATTAGATTATAGAAGTAAGCGAAAAAGTTTACCTTTTACAGAAAATGATGAAAATATTGTCGTGCTTAT
TCATCCTAAAAGTGATAAAGTAAATGCGAATGAATATTATTATGGTGAAGAAATTAAACAACAAACTGATAAAGTAGTAT
TAAGAGATTTACCAACGTCAATGGAAGACTTGTCTAATTCCTTGCAACAACTGCAATTTTCTCAACTTTATATAGTTTTG
CAACATAATCATTCGATTTACTTCGATGGTATACCTAATATGGATATTTTTAAAAAGTGTTATAAAGCATTAATAACTAA
ACAAGAAACAAATATCCAGAAAGAGGGTATGTTATTGTGTCAACATTTAAGTGTGAAACCAGATACACTTAAATTCATGT
TGAAAGTTTTCTTAGACTTAAAATTTGTAACACAAGAAGATGGTTTAATTCGAATCAATCAACAACCTGATAAAAGATCG
ATTGATTCCAGCAAAGTATATCAATTAAGACAACAACGTATGGATGTTGAAAAGCAATTATTATATCAAGATTTTTCAGA
AATAAAAAATTGGATAAAGTCACAATTGTCGTGA

Domains

Predicted by InterProScan.

RecJ OB domain (RecJ_OB)

(452-556)

DHHA1 domain (DHHA1)

(312-436)

DHH family (DHH)

(81-224)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(563-752)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

38.224

100

0.392
  recJ Streptococcus pneumoniae strain D39V

38.076

100

0.382