Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   V9W60_RS05345 Genome accession   NZ_CP146759
Coordinates   1066483..1070187 (+) Length   1234 a.a.
NCBI ID   WP_335364263.1    Uniprot ID   -
Organism   Bacillus altitudinis strain JJ61     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1061483..1075187
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V9W60_RS05330 (V9W60_05330) - 1061781..1062191 (+) 411 WP_008348626.1 DoxX family protein -
  V9W60_RS05335 (V9W60_05335) - 1062383..1062829 (-) 447 WP_007499129.1 ferritin-like domain-containing protein -
  V9W60_RS05340 (V9W60_05340) addB 1062996..1066505 (+) 3510 WP_338704861.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  V9W60_RS05345 (V9W60_05345) addA 1066483..1070187 (+) 3705 WP_335364263.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  V9W60_RS05350 (V9W60_05350) sbcD 1070213..1071388 (+) 1176 WP_335364264.1 exonuclease subunit SbcD -
  V9W60_RS05355 (V9W60_05355) sbcC 1071385..1074780 (+) 3396 WP_335364265.1 exonuclease subunit SbcC -
  V9W60_RS05360 (V9W60_05360) - 1074798..1075097 (+) 300 WP_017360217.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 142693.79 Da        Isoelectric Point: 5.1809

>NTDB_id=944223 V9W60_RS05345 WP_335364263.1 1066483..1070187(+) (addA) [Bacillus altitudinis strain JJ61]
MQIPKPNNSTWTDDQWNAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEQPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELSKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKVGKPS
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPAPEQWMRAFLSLYDVDTQTKVEELPFYPYIKEDLSLVLRSCQELLEQA
LALSKEPGGPAPRAENFIDDLEQVNELIRHQDDFGTLYELLPNINFKRLKTCKGDDYDPALLEKATDARNQAKKQLEKLK
DEYFMRSPDQHLKSLAEMKPVVETLVELVIQYGERFERAKQEKSIVDFSDLEHYCLRILAKQDAEGNLIETEAAKYYQKQ
FEEVLVDEYQDTNLVQETILKLVSKGKHPAEGNRFMVGDVKQSIYRFRLAEPMLFLNKYKQFKADSQETGKRIDLNKNFR
SRADVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPQSEDTTTEMLLVHLDQHDTESDEEREELETVQFEARVIAKK
IKELVEQPFQVYDAKQQLTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPLVHLDENEMALIRTSDKKGTYYDAVKAFMSVTHSDHPTCRKLERFFLQLRKWRDFSIQHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLIAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAANGDDWLLPDFERYQARTYLDFIGPALIRHQAMSSLLEESREVVLLHPSSFSITFHQASDL
LQEDMSLEKQKQDEVVQALMEGVPVQGYGDYEKEVKERLTWRYPYLAASQVGTKQSVSEIKRMKEIQDEYSVPASIRKAH
ATLYERPAFMKKKTLTAAEQGTAMHTVMQHIPLPSNEPYDESRVEELLHSLKEKDLLTDEQIQSINREGIVAFFSTAIGQ
KLRKADWVKREVSFSMVLPVKEVYSHIDAEGEPVLIQGMIDCLFEADGKLYLLDYKTDRVTGRFSGGLEEAEAMLKKRYE
TQISLYTAAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=944223 V9W60_RS05345 WP_335364263.1 1066483..1070187(+) (addA) [Bacillus altitudinis strain JJ61]
ATGCAAATACCAAAACCGAATAACAGTACTTGGACAGATGACCAATGGAACGCCATCGTTTCAGAAGGACAAGATATTCT
TGTTGCTGCGGCGGCAGGGTCAGGGAAAACAGCGGTCTTGGTTGAACGGCTCATTCGAAAAATGACTCGGCCGGAGCAGC
CAGTTGATGTGGACAGGCTGCTCGTAGTGACTTTTACCAATGCTTCAGCAGCAGAAATGAAGCACCGGATCACTGAAGCA
CTTGAAAAAGAACTATCTAAAAACCCTGGTTCCCTTCATATGCGGAGACAGCTGTCACTTATGAATCGTGCCAATATTTC
TACCTTGCACTCCTTTTGTTTGCAAGTTCTGCGCACTTTTTATTATGAGATTGACCTTGATCCAGGTTTTCGTTTGGCTG
ATCAAACAGAAGGAGAATTACTAGGAGATGAAGTGTTAGATGAACTGTTTGAGGATGAGTATAAGGTTGGAAAACCGTCA
TTCTTTGAACTCGTTGATCGTTACACATCTGATCGGCATGATTTAGATCTTCAATGGCTTGTCAAAAGAATTTATGAGTT
TTCTAGATCACATCCAGCGCCAGAACAGTGGATGCGGGCATTCCTTTCTCTTTATGATGTGGATACACAGACAAAGGTAG
AAGAATTACCCTTTTATCCATATATCAAAGAGGACCTTTCTCTCGTTTTAAGAAGCTGCCAGGAACTGCTGGAACAAGCG
CTCGCTTTATCAAAAGAGCCGGGCGGTCCAGCACCGAGAGCAGAGAATTTTATAGATGATTTAGAGCAAGTAAATGAATT
AATAAGGCATCAAGATGATTTTGGTACACTTTATGAGCTTTTGCCAAACATCAATTTTAAAAGGTTAAAGACTTGCAAAG
GGGATGACTATGACCCAGCTCTGTTAGAAAAAGCAACAGATGCACGCAATCAAGCCAAAAAACAATTAGAAAAGCTAAAA
GATGAATACTTTATGCGCAGTCCCGATCAGCATTTAAAAAGCTTGGCTGAAATGAAGCCAGTTGTAGAAACACTTGTAGA
GCTTGTGATTCAATATGGAGAGCGTTTCGAAAGAGCAAAACAAGAAAAATCAATCGTAGACTTTTCGGATCTAGAACATT
ATTGTTTGCGCATTTTAGCGAAGCAGGACGCAGAAGGAAATCTCATTGAAACAGAAGCGGCTAAATACTATCAAAAGCAA
TTTGAAGAAGTACTCGTGGATGAATACCAGGATACAAATCTTGTCCAAGAAACGATTTTAAAGCTCGTGTCTAAAGGGAA
ACATCCTGCAGAAGGCAACCGCTTTATGGTTGGTGATGTGAAGCAGTCCATTTATCGCTTTAGACTTGCAGAGCCGATGC
TCTTTTTAAATAAATATAAACAATTTAAAGCAGATAGCCAAGAAACAGGTAAAAGAATTGATCTAAATAAAAACTTCCGT
AGCCGGGCTGATGTATTAGACAGTACCAACTTTCTGTTCAAACAGCTGATGGGAGAAACAGTTGGAGAAATTGAATATGA
CGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCGCAAAGCGAAGATACGACGACAGAAATGCTTCTTGTTCATTTAG
ATCAGCACGACACAGAAAGCGATGAAGAACGAGAAGAGCTAGAGACGGTACAATTTGAGGCGAGAGTGATTGCAAAGAAA
ATAAAAGAATTGGTAGAACAGCCTTTTCAAGTCTACGATGCAAAACAGCAGCTGACCCGCAACTTGCAGTATCGAGACAT
TGTGATCTTGCTTCGCTCAATGCCGTGGGCTCCGCAAATGATGGAAGAGTTGAAGAAGCAGGGAATCCCTGTTTATGCGA
ATCTTTCCTCAGGCTATTTCGAAGCGACAGAGGTATCTGTCATTCTTTCTTTGTTAAAAGTGATTGATAATCCATATCAA
GATATTCCGCTCGCTGCTGTTTTAAGGTCACCTCTTGTTCATTTAGACGAAAATGAGATGGCACTGATTAGAACCAGTGA
TAAAAAAGGCACTTATTATGACGCTGTGAAGGCATTTATGAGTGTGACACACTCTGATCATCCGACATGCAGAAAGCTGG
AGCGATTCTTCCTTCAATTACGCAAATGGCGTGATTTTTCGATTCAGCATTCGGTAGCAGAACTGATTTGGGAAGTGTAC
CGAGATACACAATATCTAGATTATGTCGGCGGGATGCCAGGAGGTAAGCAAAGACAGGCAAACCTTCGGGCTTTATATGA
TCGAGCGAAACAGTATGAAAAGGCTGCATTTAGAGGACTGTTTCGATTTCTTCGATTTATTGAAAGAATGCAAGAGCGTG
GAGATGATCTTGGAGCGGCAAAAACGTTCAGTGAAACAGAGGATGTTGTCCGCATGATGACCATTCATAGCAGTAAAGGA
CTCGAATTCCCTGTTGTATTTACTGCAGGACTTGGCAGGAACTTTAACATGATGGATTTAAACCAGTCCTACTTGCTTGA
TAAAGAATTAGGTTTTGGCAGCAAATATATTCATCCTGAGCTGAGAATTAGTTATGCAACATTACCGCTGATCGCGATGA
AAAAAAAGATGAGAAAAGAGTTATTATCTGAAGAACTGAGGGTTCTATATGTGGCGCTCACAAGGGCGAAGGAAAAATTA
TTTTTAGTCGGCTCTGTCAAAAATCAAGTGAAAGCTTTAAGCAAGTGGCAAAATGCCGCAAACGGAGACGACTGGCTCCT
CCCTGATTTTGAACGATATCAAGCAAGAACATACTTAGATTTTATTGGCCCAGCCCTTATCCGTCATCAAGCGATGTCTT
CTCTTTTAGAAGAATCGAGAGAAGTCGTTCTCTTGCATCCATCATCATTTTCTATCACCTTTCATCAAGCGTCCGATCTT
CTACAAGAAGACATGTCACTTGAAAAACAAAAACAAGACGAAGTGGTACAAGCATTAATGGAAGGTGTGCCTGTTCAAGG
ATATGGCGATTACGAGAAGGAAGTAAAAGAAAGACTTACATGGCGTTATCCATATTTAGCGGCCTCCCAAGTAGGGACAA
AGCAGTCAGTTTCCGAGATCAAAAGAATGAAAGAAATACAGGATGAATACAGTGTGCCTGCTTCCATTCGAAAAGCTCAT
GCGACACTGTACGAACGACCGGCTTTTATGAAGAAAAAAACACTCACAGCCGCAGAACAAGGGACGGCCATGCATACAGT
CATGCAGCATATCCCTCTTCCTTCAAATGAACCATATGATGAATCTCGTGTAGAAGAGCTGCTCCATTCTTTAAAAGAGA
AAGACTTACTAACAGATGAACAAATCCAGTCCATCAATCGAGAAGGAATTGTGGCCTTCTTTTCTACTGCTATTGGTCAA
AAACTGCGGAAAGCGGACTGGGTGAAACGAGAAGTATCGTTTAGTATGGTTTTACCGGTTAAAGAGGTATACAGCCATAT
TGATGCAGAAGGTGAACCTGTGCTCATTCAAGGAATGATTGATTGTTTATTTGAAGCAGATGGCAAGCTCTATTTACTTG
ATTATAAAACAGATCGAGTGACTGGCAGATTCTCAGGTGGATTAGAGGAAGCTGAAGCGATGTTAAAAAAACGCTATGAA
ACGCAAATATCGTTGTATACAGCAGCGGTAGAACGTTTAACAAACAGAACATTAGAGGAGAAAATCCTTTATTTCTTTGA
TGGAAATCTAGAAATTTCTTTATAG

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-463)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1052-1226)

UvrD-like helicase C-terminal domain (UvrD_C)

(473-883)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

66.694

100

0.669