Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   V4331_RS00935 Genome accession   NZ_CP146743
Coordinates   175478..176839 (+) Length   453 a.a.
NCBI ID   WP_165711810.1    Uniprot ID   -
Organism   Lactococcus formosensis subsp. formosensis strain L5     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 170478..181839
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V4331_RS00900 (V4331_00900) rpsM 170493..170858 (+) 366 WP_003133773.1 30S ribosomal protein S13 -
  V4331_RS00905 (V4331_00905) rpsK 170876..171259 (+) 384 WP_003133774.1 30S ribosomal protein S11 -
  V4331_RS00910 (V4331_00910) - 171311..172249 (+) 939 WP_096368778.1 DNA-directed RNA polymerase subunit alpha -
  V4331_RS00915 (V4331_00915) rplQ 172265..172645 (+) 381 WP_003133776.1 50S ribosomal protein L17 -
  V4331_RS00920 (V4331_00920) - 173036..174520 (+) 1485 WP_341784224.1 S-layer homology domain-containing protein -
  V4331_RS00925 (V4331_00925) - 174530..174967 (+) 438 WP_096368776.1 hypothetical protein -
  V4331_RS00935 (V4331_00935) radA 175478..176839 (+) 1362 WP_165711810.1 DNA repair protein RadA Machinery gene
  V4331_RS00940 (V4331_00940) - 176974..178050 (+) 1077 WP_096368774.1 PIN/TRAM domain-containing protein -
  V4331_RS00945 (V4331_00945) - 178059..178688 (+) 630 WP_096368773.1 hypothetical protein -
  V4331_RS00950 (V4331_00950) - 178824..179330 (+) 507 WP_096368772.1 PepSY domain-containing protein -
  V4331_RS00955 (V4331_00955) ald 179531..180652 (+) 1122 WP_017368774.1 alanine dehydrogenase -
  V4331_RS00960 (V4331_00960) - 180683..181177 (-) 495 WP_017368775.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49713.89 Da        Isoelectric Point: 5.0724

>NTDB_id=943995 V4331_RS00935 WP_165711810.1 175478..176839(+) (radA) [Lactococcus formosensis subsp. formosensis strain L5]
MAKKKSTFLCQECGYKSVKKLGRCPNCGAWGSFVEEVEVQEVKNQRVSLTGEHSKPMKLDQVELFDTPRVETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDTDFYLYAETNMQSIRNEVE
RLQPDFLIVDSIQTIMTPEIQSTQGSVSQVREVTGELMQLAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
QNTFRILRAVKNRFGSTNEIGIFEMQGSGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRAGFLLQQQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEEPTDARDCFIGEIGLTGEIR
RVTRMEQRLNEASKLGFQKVYAPKNSLAGVNTPENLKVVGVTTLSECLKKVFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=943995 V4331_RS00935 WP_165711810.1 175478..176839(+) (radA) [Lactococcus formosensis subsp. formosensis strain L5]
ATAGCTAAAAAAAAATCAACATTTCTTTGTCAAGAATGTGGTTATAAATCAGTAAAAAAACTCGGACGTTGTCCCAATTG
TGGCGCTTGGGGCTCCTTTGTGGAAGAAGTAGAAGTCCAAGAGGTAAAAAATCAGAGAGTAAGTTTGACAGGTGAGCACT
CTAAACCCATGAAGTTAGATCAGGTTGAACTTTTTGATACACCGCGCGTGGAAACCGACCTAGATGAGTTTAACCGAGTC
CTTGGAGGCGGTGTCGTTCCAGGAAGTCTTGTCCTTATTGGGGGCGATCCAGGGATTGGTAAATCGACTCTACTGCTTCA
AGTATCTACACAACTTGCTTCAAGAGGGCGTGTCCTTTATGTGAGTGGGGAGGAGTCAGCTCAACAAATTAAGCTGCGTG
CTGAGCGTTTGGGCGACATTGATACAGATTTTTATCTTTATGCCGAAACCAATATGCAAAGTATCCGAAATGAAGTGGAA
CGTCTACAGCCAGATTTTCTTATTGTAGATTCGATTCAAACTATCATGACGCCTGAAATTCAGAGTACCCAGGGTTCAGT
TAGTCAAGTGCGTGAGGTGACAGGAGAATTAATGCAATTGGCCAAAACTAACGATATTGCGACTTTTATTGTGGGACATG
TCACCAAGGAAGGGCAACTTGCAGGCCCACGTATGTTGGAACATATGGTTGATACCGTCTTGTATTTTGAAGGAGAACGC
CAAAATACATTCCGAATTTTACGTGCGGTCAAAAACCGCTTTGGTTCAACAAATGAAATTGGAATTTTCGAGATGCAAGG
CAGTGGTTTAGTGGAAGTAACCAACCCGAGTGAAGTCTTTCTGGAAGAACGTCTCGAGGGTTCTACCGGATCTGCTATTG
TTTGTGCATTAGAAGGTACTCGTCCAATATTAGTTGAAATTCAAGCTTTGACTACACCAACCATGTTTGGGAATGCCAAA
CGTACAACCTCCGGTTTAGACTTTAACCGTGTGAGTTTAATCATGGCAGTACTTGAAAAGCGGGCCGGATTTCTTTTGCA
GCAACAAGACGCTTACTTGAAGTCAGCAGGTGGCGTTAAATTGGATGAGCCTGCAATTGACTTAGCAGTTGCAGTAGCTA
TTGCTTCTAGCTACAAAGAAGAGCCAACAGATGCACGCGACTGCTTTATTGGTGAAATTGGCTTAACTGGTGAAATCCGT
CGTGTCACACGCATGGAGCAACGTCTTAATGAAGCGAGTAAATTAGGTTTCCAAAAAGTTTATGCGCCTAAGAATAGCTT
AGCAGGCGTAAATACTCCAGAAAACTTGAAAGTGGTTGGTGTGACAACTTTATCTGAATGTTTGAAAAAAGTATTTGGTT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(77-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(359-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

80.132

100

0.801
  radA Streptococcus pneumoniae D39

80.132

100

0.801
  radA Streptococcus pneumoniae R6

80.132

100

0.801
  radA Streptococcus pneumoniae TIGR4

80.132

100

0.801
  radA Streptococcus mitis NCTC 12261

80.132

100

0.801
  radA Streptococcus mitis SK321

80.132

100

0.801
  radA Bacillus subtilis subsp. subtilis str. 168

61.438

100

0.623