Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   V6Z91_RS10480 Genome accession   NZ_CP146614
Coordinates   2364646..2366115 (+) Length   489 a.a.
NCBI ID   WP_338770118.1    Uniprot ID   -
Organism   Massilia sp. METH4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2359646..2371115
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6Z91_RS10455 - 2361061..2362083 (+) 1023 WP_338770102.1 KpsF/GutQ family sugar-phosphate isomerase -
  V6Z91_RS10460 - 2362085..2362624 (+) 540 WP_338770105.1 HAD family hydrolase -
  V6Z91_RS10465 lptC 2362642..2363235 (+) 594 WP_338770108.1 LPS export ABC transporter periplasmic protein LptC -
  V6Z91_RS10470 lptA 2363253..2363837 (+) 585 WP_338770111.1 lipopolysaccharide transport periplasmic protein LptA -
  V6Z91_RS10475 lptB 2363837..2364595 (+) 759 WP_338770114.1 LPS export ABC transporter ATP-binding protein -
  V6Z91_RS10480 rpoN/rpoN1 2364646..2366115 (+) 1470 WP_338770118.1 RNA polymerase factor sigma-54 Machinery gene
  V6Z91_RS10485 raiA 2366216..2366572 (+) 357 WP_338770121.1 ribosome-associated translation inhibitor RaiA -
  V6Z91_RS10490 - 2366706..2367788 (+) 1083 WP_338770123.1 enoyl-CoA hydratase/isomerase family protein -
  V6Z91_RS10495 - 2367796..2368773 (+) 978 WP_338770125.1 amidohydrolase family protein -
  V6Z91_RS10500 rapZ 2368870..2369733 (-) 864 WP_338770127.1 RNase adapter RapZ -
  V6Z91_RS10505 - 2369776..2371029 (-) 1254 WP_338770129.1 cation:proton antiporter -

Sequence

Protein


Download         Length: 489 a.a.        Molecular weight: 54351.87 Da        Isoelectric Point: 5.1943

>NTDB_id=943396 V6Z91_RS10480 WP_338770118.1 2364646..2366115(+) (rpoN/rpoN1) [Massilia sp. METH4]
MKQSLQLRTSQHLALTPQLQQSIRLLQLSTLELHQELEQLLTDNPLLERLDDPLDRSVRLLADGAINTSQGGTEAPPEGG
PAHDAPAAPEPEAYDGGDGEGLATGDLDWGSAGSGKGPDDDDARPQLEASHRSLREHLMEQMRVTVLESRDRALVELIID
ALDDNGYLEESLEEIHERLPEELEIEIDELRTALKLLQSFDPPGIGARNASECLALQIRRMPNVPLVTRRMALCIVEKHL
QWFAQRDFNKLKKALDCDDEDLREAQAVIRQCNPHPGAAFAPDVSDYVVPDVVVKKGRNGWVVTLNNDVMPRLRVNALYA
SLLKQGKGESQMSAQLQEAKWLIKNMRQRFDTILRVAQAIVERQKNFFSHGAVAMRPLVLREIADTLGLHESTISRVTTQ
KYMLTPHGMFELKYFFGSHVATEAGGEASSTAIRALIVQFTGAEDPKNPLSDSKIADMLGEQGMVIARRTVAKYREALKI
PPVSLRKSL

Nucleotide


Download         Length: 1470 bp        

>NTDB_id=943396 V6Z91_RS10480 WP_338770118.1 2364646..2366115(+) (rpoN/rpoN1) [Massilia sp. METH4]
ATGAAACAGTCTTTGCAACTGCGCACTTCCCAGCATCTCGCGCTGACGCCGCAACTGCAGCAGTCGATCCGGCTGCTGCA
ATTGTCCACGCTGGAGCTGCACCAGGAACTGGAACAGCTGCTGACCGATAACCCCCTGCTCGAGCGGCTCGACGATCCGC
TCGACCGCTCGGTGCGCCTGCTGGCCGATGGCGCGATCAATACCTCGCAGGGCGGCACCGAGGCGCCGCCGGAGGGCGGC
CCGGCGCACGACGCGCCGGCCGCGCCGGAACCGGAGGCGTATGACGGCGGCGATGGCGAAGGGCTGGCCACCGGCGACCT
CGACTGGGGCAGCGCCGGCAGCGGCAAGGGCCCGGACGACGACGATGCCCGCCCGCAGCTGGAAGCGAGCCACCGCAGCC
TGCGCGAGCACCTGATGGAGCAGATGCGCGTCACGGTCCTCGAATCCCGCGATCGCGCCCTGGTGGAACTGATCATCGAC
GCGCTGGACGACAACGGCTATCTCGAAGAGAGCCTGGAAGAGATCCATGAACGCTTGCCGGAAGAGCTGGAAATCGAGAT
CGACGAGTTGCGCACGGCCCTGAAACTGCTGCAGAGCTTCGACCCGCCCGGCATCGGCGCCCGCAACGCCTCCGAATGCC
TGGCGCTGCAGATCCGGCGCATGCCGAACGTGCCGCTGGTGACCCGCCGCATGGCGCTGTGCATCGTCGAAAAACACCTG
CAATGGTTCGCCCAGCGCGACTTCAACAAGCTCAAGAAGGCGCTCGATTGCGATGACGAAGACTTGCGAGAGGCGCAGGC
CGTGATTCGCCAATGCAATCCGCATCCGGGCGCCGCCTTCGCACCCGACGTGTCAGACTATGTGGTACCGGACGTGGTGG
TGAAGAAAGGACGCAACGGCTGGGTCGTCACACTGAACAACGACGTGATGCCGCGCCTGCGTGTCAATGCGCTGTATGCC
AGCCTGCTCAAGCAGGGCAAGGGCGAATCGCAGATGAGCGCCCAGTTGCAGGAAGCCAAGTGGCTCATCAAGAACATGCG
CCAGCGCTTCGACACCATCCTCCGCGTGGCCCAGGCCATCGTCGAGCGGCAGAAGAACTTTTTCTCGCATGGCGCTGTGG
CAATGAGGCCCCTTGTGTTGCGCGAGATTGCTGATACACTGGGCCTGCACGAGAGCACTATCTCTCGGGTAACAACTCAA
AAATACATGCTGACCCCTCACGGCATGTTTGAGTTGAAGTACTTTTTTGGCAGCCATGTCGCAACCGAAGCTGGTGGGGA
AGCTTCCTCGACTGCGATCAGGGCGCTGATTGTGCAATTCACAGGAGCCGAAGACCCGAAGAACCCTTTATCCGACAGTA
AGATTGCGGACATGCTGGGTGAACAAGGCATGGTGATTGCACGGCGCACCGTCGCCAAGTATCGCGAAGCATTGAAAATC
CCTCCAGTCAGCCTCCGCAAGTCTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(331-487)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(127-319)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

60.516

100

0.624