Detailed information NTG0943329

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: ACFIOO_RS04035
• Genome accession: NZ_CP170406
• Coordinates: 853159..856812 (+)
• Length: 1217 a.a.
• NCBI ID: WP_054190381.1
• Uniprot ID: -
• Organism: Staphylococcus aureus strain t21635
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 848159..861812

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACFIOO_RS04020	lepB	848407..848931 (+)	525	WP_000758209.1	signal peptidase I	-
ACFIOO_RS04025	lepB	848947..849522 (+)	576	WP_000711897.1	signal peptidase I	-
ACFIOO_RS04030	addB	849682..853158 (+)	3477	WP_054190382.1	helicase-exonuclease AddAB subunit AddB	-
ACFIOO_RS04035	addA	853159..856812 (+)	3654	WP_054190381.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
ACFIOO_RS04040	-	856978..857880 (+)	903	WP_000670752.1	fumarylacetoacetate hydrolase family protein	-
ACFIOO_RS04045	-	858012..859142 (+)	1131	WP_223810117.1	Fic family protein	-
ACFIOO_RS04050	-	859438..859827 (+)	390	WP_000902813.1	YisL family protein	-
ACFIOO_RS04055	-	859998..861314 (-)	1317	WP_054191858.1	CoA-disulfide reductase	-

Sequence

Protein

• Length: 1217 a.a.
• Molecular weight: 141289.86 Da
• Isoelectric Point: 4.9274

>NTDB_id=943329 ACFIOO_RS04035 WP_054190381.1 853159..856812(+) (addA) [Staphylococcus aureus strain t21635]
MTIPEKPQGVIWTDAQWQSIYATGQDVLVAAAAGSGKTAVLVERIIQKILRDGIDVDRLLVVTFTNLSAREMKHRVDQRI
QEASIADPANAHLKNQRIKIHQAQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEVIEQHYDILDPAF
IELTEQLSSDRSDDQFRMIIKQLYFFSVANPNPTNWLDQLVTPYEEEAQQAQLIQLLTDLSKVFVTAAYDALNKAYDLFS
MMDGVDKHLVVIEDERRLMGRVLESGFIDIPYLTGHEFGARLPNVTAKIKEANEMMVDALEDAKLQYKKYKSLIDKVKSD
YFSREADDLKADMQQLAPRVKYLARIVKDVMSEFNRKKRSKNILDFSDYEHFALQILTNEDGSPSEIAESYRQHFQEILV
DEYQDTNRVQEKILSCIKTGDEHNGNLFMVGDVKQSIYKFRQADPSLFIEKYQRFTIDGDGTGRRIDLSQNFRSRKEVLS
TTNYIFKHMMDEQVGEVKYDESAQLYYGAPYDESNHPVNLKVLVEADQEHSDLTGSEQEAHFIVEQVKDILEHQKVYDMK
TESYRSATFKDIVILERSFGQARNLQQAFKNEDIPFHVNSREGYFEQTEVRLVLSFLRAIDNPLQDIYLVGLMRSVIYQF
KEDELAQIRILSPNDDYFYQSIVNYINDEAADAILVDKLKMFLSDIQSYQQYSKDHPVYQLIDKFYNDHYVIQYFSGLIG
GRGRRANLYGLFNKAIEFENSSFRGLYQFIRFIDELIERGKDFGEENVVGPNDNVVRMMTIHSSKGLEFPFVIYSGLSKD
FNKRDLKQPVILNQQFGLGMDYFDVDKEMAFPSLASVAYKAVAEKELVSEEMRLVYVALTRAKEQLYLIGRVKNDKSLLE
LEQLSISGEHIAVNERLTSPNPFHLIYSILSKHQSASIPDDLKFEKDIAQVEDSSRPNVNISIIYFEDVSTETILDNDEY
RSVNQLETMQNGNEDVKAQIKHQLDYQYPYVNDTKKPSKQSVSELKRQYETEESGTSYERVRQYRIGFSTYERPKFLSEQ
GKRKANEIGTLMHTVMQHLPFKKERISEVELHQYIDGLIDKHIIEADAKKDIRMDEIMTFINSELYSIIAEAEQVYRELP
FVVNQALVDQLPQGDEDVSIIQGMIDLIFVKDGVHYFVDYKTDAFNRRRGMTDEEIGTQLKNKYKIQMKYYQNTLQTILN
KEVKGYLYFFKFGTLQL

Nucleotide

• Length: 3654 bp

>NTDB_id=943329 ACFIOO_RS04035 WP_054190381.1 853159..856812(+) (addA) [Staphylococcus aureus strain t21635]
ATGACAATTCCAGAGAAACCACAAGGCGTGATTTGGACTGACGCGCAATGGCAAAGTATTTACGCAACCGGACAAGATGT
ACTTGTTGCAGCCGCGGCAGGTTCAGGTAAAACAGCTGTACTAGTTGAGCGTATTATCCAAAAGATTTTACGTGATGGCA
TTGATGTCGATCGACTTTTAGTCGTAACGTTTACAAACTTAAGTGCACGTGAAATGAAGCATCGTGTAGACCAACGTATT
CAAGAGGCATCGATTGCTGATCCTGCAAATGCACACTTGAAAAACCAACGCATCAAAATTCATCAAGCACAAATATCTAC
ACTTCATAGTTTTTGCTTGAAATTAATTCAACAGCATTACGATGTATTAAATATTGACCCGAACTTTAGAACAAGCAGTG
AAGCTGAAAATATTTTATTATTAGAACAAACGATAGATGAAGTCATAGAACAACATTACGATATCCTTGATCCTGCTTTT
ATTGAATTAACAGAGCAATTGTCTTCAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAG
CGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTGACACCATACGAAGAAGAAGCACAACAAGCCCAACTTA
TTCAACTACTAACAGACTTATCTAAAGTATTTGTTACAGCTGCATATGATGCTTTAAATAAGGCGTATGATTTGTTTAGT
ATGATGGATGGTGTCGATAAACATTTAGTTGTTATAGAAGATGAACGACGTTTAATGGGGCGTGTTTTAGAAAGTGGCTT
TATTGATATACCTTATTTAACTGGTCACGAATTTGGCGCGCGTTTGCCTAATGTAACAGCGAAAATTAAAGAAGCAAATG
AAATGATGGTCGATGCCTTAGAAGATGCTAAACTTCAGTATAAAAAATATAAATCATTAATTGATAAAGTGAAAAGTGAT
TATTTTTCAAGAGAAGCTGATGATTTGAAAGCTGATATGCAACAGTTGGCACCACGAGTAAAGTACCTTGCGCGTATAGT
GAAAGATGTTATGTCAGAATTCAATCGAAAAAAGCGTAGCAAAAATATTTTGGATTTTTCTGATTATGAACATTTTGCAT
TACAAATTTTAACTAATGAGGATGGTTCGCCTTCAGAAATTGCCGAATCATACCGTCAACACTTCCAAGAAATATTGGTC
GATGAGTATCAAGATACGAACCGAGTTCAAGAGAAAATACTTTCTTGCATCAAAACGGGTGATGAACATAATGGTAATTT
ATTTATGGTTGGAGATGTTAAGCAATCCATTTATAAATTTAGACAAGCTGATCCAAGTTTATTTATTGAAAAGTATCAAC
GGTTTACTATAGATGGAGATGGCACTGGACGTCGAATTGATTTATCACAAAACTTCCGTTCTCGTAAAGAAGTACTGTCA
ACGACTAACTATATATTCAAACATATGATGGATGAACAAGTCGGTGAAGTAAAATATGATGAATCAGCACAATTGTATTA
TGGTGCACCATATGATGAATCCAATCATCCAGTTAACTTAAAGGTGCTTGTTGAAGCGGATCAAGAACATAGTGATTTAA
CTGGTAGTGAACAAGAAGCGCATTTTATAGTTGAACAAGTTAAAGATATCTTAGAACATCAAAAAGTTTATGATATGAAG
ACAGAAAGCTATAGAAGTGCGACATTCAAGGATATCGTTATTCTAGAACGCAGCTTTGGACAAGCTCGCAATTTACAACA
AGCTTTTAAAAATGAAGATATTCCATTCCATGTGAATAGTCGTGAAGGTTACTTTGAACAAACAGAAGTCCGTTTAGTAT
TATCATTTTTAAGAGCGATAGATAATCCATTACAAGATATTTATTTAGTTGGGTTAATGCGCTCCGTTATATATCAGTTC
AAAGAAGACGAATTAGCTCAAATTAGAATATTGAGTCCAAATGATGACTACTTCTATCAATCGATTGTAAATTACATTAA
TGATGAAGCAGCAGATGCTATTTTAGTTGATAAATTAAAAATGTTTTTATCAGATATTCAAAGTTACCAACAATATAGTA
AAGATCATCCGGTGTATCAGTTAATTGATAAATTTTATAATGATCATTATGTTATTCAATACTTTAGTGGACTTATTGGT
GGACGTGGACGACGTGCAAATCTTTATGGTTTATTTAATAAAGCTATCGAGTTTGAGAATTCAAGTTTTAGAGGTTTATA
TCAATTTATTCGTTTTATCGATGAATTGATTGAAAGAGGCAAAGATTTTGGTGAGGAAAATGTAGTTGGTCCAAACGATA
ATGTCGTTAGAATGATGACAATTCATAGTAGTAAAGGTCTAGAGTTTCCATTTGTCATTTATTCTGGATTGTCAAAAGAT
TTTAATAAACGTGATTTGAAACAACCAGTTATTTTAAATCAGCAATTTGGTCTCGGAATGGATTATTTTGATGTGGATAA
AGAAATGGCATTTCCGTCTTTAGCTTCGGTTGCATATAAAGCTGTTGCCGAAAAAGAACTTGTGTCAGAAGAAATGCGAT
TAGTCTATGTAGCATTAACGAGAGCGAAAGAACAACTTTATTTAATTGGTAGAGTGAAAAATGATAAATCGTTACTAGAA
CTAGAGCAATTGTCTATTTCTGGTGAGCACATTGCTGTCAATGAACGATTAACTTCACCAAATCCGTTCCATCTTATTTA
TAGTATTTTATCTAAACATCAATCTGCGTCAATTCCGGATGATTTAAAATTTGAAAAAGATATAGCACAAGTTGAAGATA
GTAGTCGTCCGAATGTAAATATTTCAATTATATACTTTGAGGATGTGTCTACAGAAACCATTTTAGATAATGATGAATAT
CGTTCGGTTAATCAATTAGAAACTATGCAAAATGGTAATGAAGATGTTAAAGCACAAATTAAACACCAACTTGATTATCA
ATATCCATATGTAAATGATACTAAAAAGCCCTCAAAACAATCTGTTTCTGAATTGAAAAGGCAATATGAAACAGAAGAAA
GTGGCACAAGTTACGAACGAGTAAGGCAATATCGTATCGGTTTTTCAACGTATGAACGACCTAAATTTCTAAGTGAACAA
GGTAAACGAAAAGCGAATGAAATTGGTACGTTAATGCATACAGTTATGCAACATTTACCATTCAAAAAAGAACGCATATC
TGAAGTTGAGTTACATCAGTATATCGATGGATTAATCGATAAACATATTATCGAAGCAGATGCGAAAAAAGATATCCGTA
TGGATGAAATAATGACATTTATCAATAGTGAGTTATATTCGATTATTGCTGAAGCAGAGCAAGTTTATCGTGAATTACCG
TTTGTAGTTAACCAAGCATTAGTTGACCAATTGCCACAAGGAGACGAAGACGTCTCAATTATTCAAGGTATGATTGACTT
GATCTTTGTTAAAGACGGTGTGCATTATTTTGTAGACTATAAAACCGATGCATTTAATCGTCGCCGTGGGATGACAGATG
AAGAAATTGGTACACAATTAAAAAATAAATATAAGATACAGATGAAATATTATCAAAATACGCTTCAAACGATTCTTAAT
AAAGAAGTTAAAGGTTATTTATACTTCTTCAAATTTGGTACATTGCAACTGTAG

Domains

Predicted by InterProScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (466-869)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1040-1210)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (12-455)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	36.759	100	0.382