Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   V8J79_RS14065 Genome accession   NZ_CP146376
Coordinates   2997637..2999145 (+) Length   502 a.a.
NCBI ID   WP_047899049.1    Uniprot ID   -
Organism   Burkholderia pyrrocinia strain JBC226     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2992637..3004145
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V8J79_RS14040 (V8J79_14040) kdsD 2993880..2994863 (+) 984 WP_047899044.1 arabinose 5-phosphate isomerase KdsD -
  V8J79_RS14045 (V8J79_14045) - 2994863..2995399 (+) 537 WP_047899045.1 HAD family hydrolase -
  V8J79_RS14050 (V8J79_14050) lptC 2995403..2996017 (+) 615 WP_338638547.1 LPS export ABC transporter periplasmic protein LptC -
  V8J79_RS14055 (V8J79_14055) lptA 2996038..2996703 (+) 666 WP_047899047.1 lipopolysaccharide transport periplasmic protein LptA -
  V8J79_RS14060 (V8J79_14060) lptB 2996700..2997476 (+) 777 WP_047899048.1 LPS export ABC transporter ATP-binding protein -
  V8J79_RS14065 (V8J79_14065) rpoN/rpoN1 2997637..2999145 (+) 1509 WP_047899049.1 RNA polymerase factor sigma-54 Machinery gene
  V8J79_RS14070 (V8J79_14070) raiA 2999303..2999662 (+) 360 WP_006477786.1 ribosome-associated translation inhibitor RaiA -
  V8J79_RS14075 (V8J79_14075) - 2999832..3000371 (+) 540 WP_011353226.1 PTS sugar transporter subunit IIA -
  V8J79_RS14080 (V8J79_14080) hprK 3000501..3001469 (+) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  V8J79_RS14085 (V8J79_14085) rapZ 3001545..3002453 (+) 909 WP_338638548.1 RNase adapter RapZ -
  V8J79_RS14090 (V8J79_14090) - 3002481..3003116 (+) 636 WP_065499174.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 54829.90 Da        Isoelectric Point: 4.9300

>NTDB_id=942803 V8J79_RS14065 WP_047899049.1 2997637..2999145(+) (rpoN/rpoN1) [Burkholderia pyrrocinia strain JBC226]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPGQNTDPAP
ANSGSSTSGDRAERDERAGADDYDGYGSGDGNDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAS
PRDRALVMFLIESLDDDGYLTATLDEVLADLPSELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRT
LALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVV
PRLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTL
GLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVA
RRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=942803 V8J79_RS14065 WP_047899049.1 2997637..2999145(+) (rpoN/rpoN1) [Burkholderia pyrrocinia strain JBC226]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTCGCCGCGGACGGATCGCTGATCGCGCAGACACCTCCCGGCCAGAACACCGATCCGGCCCCG
GCAAACAGCGGCAGCAGCACGTCGGGCGATCGCGCGGAGCGCGACGAGCGGGCCGGTGCCGACGACTACGACGGTTATGG
GTCCGGCGACGGCAACGACGGCCCGCAATGGAATCTCGACGAATTCGGCCGCTCGAGCGGCGCGTCCGACGACGACGACC
TCCCGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGTTGCGCGTCACGCAGGCCAGC
CCGCGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAAGT
GCTCGCCGACCTGCCGTCCGAGCTGGAGATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACC
CGGCCGGGGTCGGCGCCCGCTCGGCATCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCACCGACGCGCACG
CTCGCGCTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCACGCGACTTCACGCGGTTGCGCAAGCACCTGAAGGC
GAGCGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCGTACGGCAAGGCCG
AGGCCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCGTG
CCGCGCCTGCGGATCAACAACCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCATCCGCCGGTTCGCTGAAGCA
GCAGCTCCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGTTTCGACACGATCCTGCGTGTCGCGCAGGCTATTG
TCGAGCGTCAGAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTG
GGCCTACACGAGTCGACTGTCAGCCGTGTGACAACAGGCAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTA
CTTCTTCGGATCACACGTTTCAACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTGATCAAGCAACTGA
TAGGAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCG
CGCCGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-500)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.379

100

0.689