Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   V6986_RS11245 Genome accession   NZ_CP146358
Coordinates   2240066..2241136 (-) Length   356 a.a.
NCBI ID   WP_084096410.1    Uniprot ID   -
Organism   Bacillus safensis strain yw6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2235066..2246136
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6986_RS11195 (V6986_11195) - 2235159..2235731 (-) 573 WP_047202287.1 signal peptidase I -
  V6986_RS11200 (V6986_11200) tapA 2235783..2236310 (-) 528 WP_151284417.1 amyloid fiber anchoring/assembly protein TapA -
  V6986_RS11205 (V6986_11205) - 2236610..2236888 (+) 279 WP_064497648.1 DUF3889 domain-containing protein -
  V6986_RS11210 (V6986_11210) - 2236922..2237116 (-) 195 WP_330545301.1 YqzE family protein -
  V6986_RS11215 (V6986_11215) comGG 2237176..2237559 (-) 384 WP_338591691.1 competence type IV pilus minor pilin ComGG -
  V6986_RS11220 (V6986_11220) comGF 2237556..2238086 (-) 531 WP_338591693.1 competence type IV pilus minor pilin ComGF -
  V6986_RS11225 (V6986_11225) comGE 2237995..2238312 (-) 318 WP_058839085.1 competence type IV pilus minor pilin ComGE -
  V6986_RS11230 (V6986_11230) comGD 2238296..2238742 (-) 447 WP_058839084.1 competence type IV pilus minor pilin ComGD -
  V6986_RS11235 (V6986_11235) comGC 2238735..2239028 (-) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  V6986_RS11240 (V6986_11240) comGB 2239045..2240085 (-) 1041 WP_338591697.1 competence type IV pilus assembly protein ComGB -
  V6986_RS11245 (V6986_11245) comGA 2240066..2241136 (-) 1071 WP_084096410.1 competence type IV pilus ATPase ComGA Machinery gene
  V6986_RS11250 (V6986_11250) - 2241314..2242189 (-) 876 WP_039179956.1 STAS domain-containing protein -
  V6986_RS11260 (V6986_11260) - 2242414..2242794 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  V6986_RS11265 (V6986_11265) - 2243010..2243252 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  V6986_RS11270 (V6986_11270) - 2243300..2243938 (-) 639 WP_034280195.1 MBL fold metallo-hydrolase -
  V6986_RS11275 (V6986_11275) - 2244096..2244269 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  V6986_RS11280 (V6986_11280) - 2244457..2244771 (-) 315 WP_338591706.1 MTH1187 family thiamine-binding protein -
  V6986_RS11285 (V6986_11285) - 2244848..2246101 (-) 1254 WP_047202298.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40370.87 Da        Isoelectric Point: 9.4005

>NTDB_id=942744 V6986_RS11245 WP_084096410.1 2240066..2241136(-) (comGA) [Bacillus safensis strain yw6]
MYGIEYLRQELLEEACRMRASDVHIVPKEKEALVSFRVDSDLIQQRTIDKKTGERLIAHFKFLSSMDIGEKRRPQNGSLA
VVLRSGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASARLLSFLKHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVLSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELTCPFCGETCQLYCKLNRPIRRTNVFELLFGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKNTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=942744 V6986_RS11245 WP_084096410.1 2240066..2241136(-) (comGA) [Bacillus safensis strain yw6]
TTGTATGGGATTGAATATTTAAGGCAAGAGCTGCTAGAAGAAGCATGCCGCATGAGAGCATCTGATGTGCACATTGTCCC
AAAAGAAAAGGAAGCGTTGGTATCTTTCCGCGTAGACTCAGATTTAATCCAGCAGCGAACCATTGATAAAAAAACCGGCG
AGCGGCTCATTGCTCATTTTAAATTTTTATCTTCTATGGATATCGGGGAAAAAAGGAGACCGCAAAATGGGTCATTAGCT
GTGGTGCTAAGAAGCGGCCAGGTGTTTATTCGAATGTCTACCTTACCAACTGTAAATGATGAGAGTTTAGTGATTAGAAT
ATTGCCGCAGGATCATGTTCCGAAAATAAAACACCTGTCACTATTTCCGAAAGCTTCAGCCAGGTTATTGTCATTTTTGA
AGCATTCGCATGGGCTTATTTTATTTACCGGTCCAACCAATTCGGGGAAAACAACAACCCTCTATTCACTGATCCAGTTT
GCAAAAAAGAATTTCAACCGAAATATTATTACGCTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGCTGCAGGTTCA
AGTAAATGAAAAAGCCGGCATCACATATGCCGCAGGATTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTAG
GAGAAATAAGAGATGCTGAAACGGCCAGAACGGCGATTAGAGCAGCACTGACAGGCCATTTAGTATTGAGTACGCTCCAT
GCAAAAAATGCAAAAGGAGCCCTTTATCGTATGCTTGAATTTGGTGTCACGATGAATGAACTTGAGCAAACGATGGTTGC
GATTGCTGCCCAGCGATTAATCGAGCTCACCTGTCCTTTTTGCGGAGAAACATGTCAGCTTTACTGTAAATTAAATCGAC
CGATCAGACGAACAAATGTATTTGAACTGCTATTCGGAAAAGAGCTTGGTGAGTGTATCAAGGAGGCTAAAGGAGAATAT
GCTCACTCGTCATATGAAACACTGCAAAGATTAATTCGTAAAGGAGTGGCACTCGGCTATTTATCAAAAAACACTTACCA
TCGCTGGGTTTATGAAGAAGCGAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

65.254

99.438

0.649