Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   V8L73_RS15325 Genome accession   NZ_CP146357
Coordinates   3315956..3317392 (+) Length   478 a.a.
NCBI ID   WP_027274871.1    Uniprot ID   -
Organism   Leminorella grimontii strain LG-KP-E1-2-T0     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3310956..3322392
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V8L73_RS15295 (V8L73_15295) mlaF 3311190..3311993 (-) 804 WP_261832028.1 phospholipid ABC transporter ATP-binding protein MlaF -
  V8L73_RS15300 (V8L73_15300) kdsD 3312497..3313465 (+) 969 WP_347253376.1 arabinose-5-phosphate isomerase KdsD -
  V8L73_RS15305 (V8L73_15305) kdsC 3313519..3314067 (+) 549 WP_261832027.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  V8L73_RS15310 (V8L73_15310) lptC 3314064..3314651 (+) 588 WP_027274874.1 LPS export ABC transporter periplasmic protein LptC -
  V8L73_RS15315 (V8L73_15315) lptA 3314641..3315159 (+) 519 WP_027274873.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  V8L73_RS15320 (V8L73_15320) lptB 3315206..3315931 (+) 726 WP_027274872.1 LPS export ABC transporter ATP-binding protein -
  V8L73_RS15325 (V8L73_15325) rpoN/rpoN1 3315956..3317392 (+) 1437 WP_027274871.1 RNA polymerase factor sigma-54 Machinery gene
  V8L73_RS15330 (V8L73_15330) ptsN 3317410..3317949 (+) 540 WP_051155726.1 PTS IIA-like nitrogen regulatory protein PtsN -
  V8L73_RS15335 (V8L73_15335) rapZ 3318119..3318991 (+) 873 WP_027274870.1 RNase adapter RapZ -
  V8L73_RS15340 (V8L73_15340) rnk 3319258..3319665 (+) 408 WP_027274869.1 nucleoside diphosphate kinase regulator -
  V8L73_RS15345 (V8L73_15345) pmbA 3319750..3321090 (-) 1341 WP_027274868.1 metalloprotease PmbA -
  V8L73_RS15350 (V8L73_15350) yjgA 3321235..3321792 (+) 558 WP_027274867.1 ribosome biogenesis factor YjgA -

Sequence

Protein


Download         Length: 478 a.a.        Molecular weight: 53695.74 Da        Isoelectric Point: 4.4068

>NTDB_id=942704 V8L73_RS15325 WP_027274871.1 3315956..3317392(+) (rpoN/rpoN1) [Leminorella grimontii strain LG-KP-E1-2-T0]
MKQGLQLRLSQQLTMTPQLQQAIRLLQLSTLELQQEIQTALESNPLLEQADAHDEIDAREEPEMENIDTSDALQQSDIPE
DLPLDASWDEIYTAGTPSGTRNDYGDDELPVFQGETTLTLQDHLIWQMDLTPFTELDKAIAVAIIDAVDDAGYLTVPLED
IAESLGQEDEAVDIAEIEAVLKRIQRFDPVGVAARDLRECLLVQLSQLDKNTPGLTQACEIVSEHLTLLGNHDFRTLLKL
TRISESLLKEAMQLIQSLDPRPGQSIYASESEYVIPDVLVRKIQGKWCVELNTESVPRLRINQHYAAMTNTHNASDNQYI
RSHLQDAKWLIKSLESRNDTLLRVTECIVERQEAFFESGPEFMKPMVLADIAQAVDMHESTISRVTTQKYLHSPRGIFEL
KYFFSSHVSTESGGEASSTAIRALVKKMISAENPSKPLSDSKIADMLTEQGIMVARRTVAKYRESLSIPPSNLRKQLV

Nucleotide


Download         Length: 1437 bp        

>NTDB_id=942704 V8L73_RS15325 WP_027274871.1 3315956..3317392(+) (rpoN/rpoN1) [Leminorella grimontii strain LG-KP-E1-2-T0]
ATGAAGCAAGGTTTACAGCTCAGGCTAAGCCAACAGTTAACCATGACGCCCCAACTACAGCAGGCCATTCGTCTGCTGCA
GCTGTCGACGCTTGAGCTACAGCAGGAGATTCAAACCGCGCTGGAGAGCAACCCGCTGCTTGAACAGGCTGACGCCCACG
ACGAAATCGACGCGCGCGAAGAGCCCGAGATGGAGAATATTGACACCAGCGACGCGCTGCAACAGTCGGATATTCCCGAA
GACCTCCCGCTGGACGCCTCCTGGGACGAAATCTACACCGCAGGTACGCCTTCCGGCACCCGCAACGACTACGGGGACGA
CGAGCTGCCCGTCTTTCAGGGCGAGACCACGCTGACGCTTCAGGACCATCTGATATGGCAAATGGATCTCACCCCCTTTA
CCGAGCTGGATAAGGCTATCGCCGTCGCCATCATCGACGCCGTCGACGATGCGGGCTATCTTACCGTCCCGCTGGAAGAC
ATCGCCGAAAGCCTGGGGCAGGAAGATGAAGCGGTAGACATCGCAGAAATTGAGGCGGTGCTTAAGCGCATTCAACGCTT
CGATCCGGTCGGCGTCGCCGCGCGCGACCTGCGCGAATGCCTGCTGGTGCAGCTATCCCAGCTGGATAAAAACACCCCGG
GGCTTACGCAGGCGTGTGAGATCGTTTCAGAGCACTTGACCCTGCTGGGCAATCACGACTTTAGAACGCTGCTAAAACTG
ACTCGAATCAGCGAATCGTTGCTAAAAGAAGCGATGCAGCTTATTCAAAGCCTCGATCCGCGCCCCGGCCAATCGATATA
CGCCAGCGAATCGGAATACGTCATTCCCGACGTCCTAGTGCGTAAAATTCAGGGGAAATGGTGCGTGGAGCTCAATACGG
AAAGCGTTCCGCGGCTTCGCATCAACCAGCACTACGCCGCCATGACCAACACGCATAACGCCAGCGATAATCAGTATATC
CGCAGCCATTTGCAGGACGCAAAGTGGCTGATAAAAAGCCTGGAAAGCCGTAACGATACGCTGCTACGGGTAACGGAATG
CATCGTGGAGCGCCAAGAGGCCTTCTTCGAGTCAGGGCCGGAATTTATGAAGCCCATGGTTTTAGCAGACATCGCCCAGG
CCGTTGATATGCACGAGTCCACCATCTCACGGGTGACTACGCAAAAATATCTGCACAGCCCGCGCGGCATTTTTGAGCTA
AAATACTTCTTCTCCAGCCATGTGAGCACCGAAAGCGGCGGCGAAGCCTCCTCTACCGCCATCCGTGCGCTGGTGAAAAA
AATGATCTCGGCCGAGAATCCCTCCAAGCCGCTCAGCGACAGTAAAATCGCCGATATGCTAACGGAACAGGGAATTATGG
TCGCCAGAAGGACCGTGGCAAAATACCGAGAGTCGTTGTCCATTCCTCCCTCAAACCTGCGTAAGCAGCTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(117-305)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(318-476)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.455

100

0.481