Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   V8Z81_RS23040 Genome accession   NZ_CP146257
Coordinates   4412803..4413855 (-) Length   350 a.a.
NCBI ID   WP_013059177.1    Uniprot ID   A0A8D4BHW7
Organism   Priestia megaterium strain MLY91     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4407803..4418855
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V8Z81_RS23000 (V8Z81_23000) - 4408818..4409642 (+) 825 WP_013059169.1 YqhG family protein -
  V8Z81_RS23005 (V8Z81_23005) - 4409667..4409852 (-) 186 WP_013059170.1 YqzE family protein -
  V8Z81_RS23010 (V8Z81_23010) comGG 4409896..4410270 (-) 375 WP_013059171.1 competence type IV pilus minor pilin ComGG -
  V8Z81_RS23015 (V8Z81_23015) comGF 4410260..4410697 (-) 438 WP_053487735.1 competence type IV pilus minor pilin ComGF -
  V8Z81_RS23020 (V8Z81_23020) - 4410694..4411032 (-) 339 WP_013059173.1 type II secretion system protein -
  V8Z81_RS23025 (V8Z81_23025) comGD 4411016..4411453 (-) 438 WP_014458229.1 competence type IV pilus minor pilin ComGD -
  V8Z81_RS23030 (V8Z81_23030) comGC 4411440..4411751 (-) 312 WP_013059175.1 competence type IV pilus major pilin ComGC -
  V8Z81_RS23035 (V8Z81_23035) comGB 4411766..4412812 (-) 1047 WP_013059176.1 competence type IV pilus assembly protein ComGB -
  V8Z81_RS23040 (V8Z81_23040) comGA 4412803..4413855 (-) 1053 WP_013059177.1 competence type IV pilus ATPase ComGA Machinery gene
  V8Z81_RS23045 (V8Z81_23045) - 4414125..4414508 (-) 384 WP_013059178.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  V8Z81_RS23050 (V8Z81_23050) - 4414770..4415006 (+) 237 WP_013059179.1 DUF2626 domain-containing protein -
  V8Z81_RS23055 (V8Z81_23055) - 4415044..4415676 (-) 633 WP_014458225.1 MBL fold metallo-hydrolase -
  V8Z81_RS23060 (V8Z81_23060) - 4415885..4416061 (+) 177 WP_013059181.1 DUF2759 domain-containing protein -
  V8Z81_RS23065 (V8Z81_23065) - 4416097..4416999 (-) 903 WP_338788684.1 M14 family metallocarboxypeptidase -
  V8Z81_RS23070 (V8Z81_23070) - 4417111..4418079 (-) 969 WP_013059183.1 ROK family glucokinase -
  V8Z81_RS23075 (V8Z81_23075) - 4418082..4418288 (-) 207 WP_013059184.1 YqgQ family protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39625.02 Da        Isoelectric Point: 9.0586

>NTDB_id=942445 V8Z81_RS23040 WP_013059177.1 4412803..4413855(-) (comGA) [Priestia megaterium strain MLY91]
MTEIEQFATYIIRDACECRSSDVHIVPRKKDAVIQLRIDQELILKRVISKEMSERLISHFKFLGGMDIGERRKPQNGALT
TSIDHYQVNLRLSTLPTSHDESLVIRLLPHQQSTSLKHLSLFPEHTKKLISLLKFSHGLIIFTGPTGSGKTTTLYTLLHE
VKHLFNRNVITLEDPIEKPSETVLQVQVNERAGITYTTGLKAILRHDPDIIMVGEIRDSETAKIAVRAALTGHLVLTTMH
TKDTKGSLYRLLEFGVSFQEMEQTLVAVAAQRLVEIRCPLCSGKCHPACKKMRKHRQSSIYELLYGKELSAVMREVKGEN
ADYDYKTLRDVILKGIALGYLYPHSLDGWG

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=942445 V8Z81_RS23040 WP_013059177.1 4412803..4413855(-) (comGA) [Priestia megaterium strain MLY91]
TTGACCGAAATTGAACAGTTTGCAACGTACATTATACGAGATGCATGTGAGTGCCGAAGTTCTGATGTGCATATTGTGCC
CCGAAAGAAAGATGCTGTGATTCAGCTGCGAATTGATCAGGAGCTTATTTTAAAGAGGGTTATTTCAAAGGAAATGTCTG
AACGGCTCATTTCGCATTTTAAATTTTTAGGGGGAATGGATATTGGCGAGCGCAGAAAACCTCAAAATGGTGCATTGACC
ACTTCCATTGATCACTATCAAGTAAATTTGCGTTTATCAACCCTTCCTACTTCTCATGATGAAAGTCTTGTTATTCGGCT
TTTGCCTCATCAACAATCTACATCTCTCAAACATTTATCTCTTTTTCCTGAACACACTAAAAAGCTTATTTCTCTCTTGA
AATTCTCTCATGGATTAATTATTTTTACCGGTCCTACAGGGTCAGGTAAAACCACTACTCTATATACTCTTTTACACGAA
GTAAAGCACCTTTTTAATCGAAATGTTATTACACTAGAAGATCCTATTGAAAAACCGAGTGAAACCGTGCTTCAAGTACA
GGTAAATGAACGGGCGGGGATTACGTACACGACTGGTTTAAAAGCTATATTGCGCCATGACCCAGACATTATCATGGTGG
GAGAAATAAGAGATAGTGAGACTGCCAAAATTGCTGTGAGAGCAGCGCTTACCGGACACTTGGTTTTGACAACGATGCAT
ACTAAAGATACAAAAGGATCTTTGTACAGGCTGCTTGAATTTGGGGTTAGCTTTCAAGAAATGGAACAGACTCTTGTAGC
AGTAGCTGCACAGCGCCTTGTAGAAATTCGCTGTCCGCTGTGTTCCGGAAAATGCCACCCCGCTTGTAAAAAAATGAGAA
AGCATCGTCAGTCCAGCATATACGAATTGCTTTATGGCAAGGAGCTTAGTGCAGTGATGAGAGAAGTGAAAGGAGAAAAT
GCGGATTATGATTATAAGACGCTCCGAGATGTGATTCTAAAAGGAATCGCGCTAGGCTATTTATATCCGCATTCGCTAGA
CGGATGGGGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.571

100

0.586