Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   V8Z81_RS19925 Genome accession   NZ_CP146257
Coordinates   3801054..3801428 (+) Length   124 a.a.
NCBI ID   WP_228540951.1    Uniprot ID   -
Organism   Priestia megaterium strain MLY91     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3796054..3806428
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V8Z81_RS19905 (V8Z81_19905) - 3796545..3797216 (-) 672 WP_098322305.1 response regulator transcription factor -
  V8Z81_RS19910 (V8Z81_19910) - 3797542..3798360 (+) 819 WP_013058559.1 phytoene/squalene synthase family protein -
  V8Z81_RS19915 (V8Z81_19915) - 3798396..3800345 (-) 1950 WP_053487648.1 FUSC family protein -
  V8Z81_RS19920 (V8Z81_19920) - 3800501..3800761 (-) 261 WP_013058561.1 metal-sensitive transcriptional regulator -
  V8Z81_RS19925 (V8Z81_19925) nucA/comI 3801054..3801428 (+) 375 WP_228540951.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  V8Z81_RS19930 (V8Z81_19930) comJ 3801464..3801871 (+) 408 WP_029323752.1 competence protein ComJ -
  V8Z81_RS19935 (V8Z81_19935) helD 3801900..3804218 (-) 2319 WP_013058564.1 RNA polymerase recycling motor HelD -
  V8Z81_RS19940 (V8Z81_19940) - 3804463..3804588 (-) 126 WP_016765178.1 hypothetical protein -
  V8Z81_RS19945 (V8Z81_19945) - 3804728..3805246 (-) 519 WP_223268980.1 enoyl-CoA hydratase/isomerase family protein -
  V8Z81_RS19950 (V8Z81_19950) - 3805486..3805854 (+) 369 WP_029323759.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 124 a.a.        Molecular weight: 13589.84 Da        Isoelectric Point: 4.3135

>NTDB_id=942439 V8Z81_RS19925 WP_228540951.1 3801054..3801428(+) (nucA/comI) [Priestia megaterium strain MLY91]
MGHAAFLAPEKTAAEVNYDATLYFPLDRYPETGDHIRDAIAAGHSSVCTVDRDGAEANREESLKGYPTKTGYDRDEWPMA
MCEEGGAGADIRYISPSDNRGAGSWVGNQLEQYPDGTRVQFIVQ

Nucleotide


Download         Length: 375 bp        

>NTDB_id=942439 V8Z81_RS19925 WP_228540951.1 3801054..3801428(+) (nucA/comI) [Priestia megaterium strain MLY91]
ATGGGACATGCAGCTTTTTTAGCTCCTGAAAAAACGGCAGCTGAAGTAAACTACGATGCAACGCTTTATTTTCCGTTAGA
CCGATATCCTGAAACGGGTGATCATATTCGTGACGCGATTGCCGCTGGGCACTCTTCCGTTTGCACAGTTGATCGCGACG
GTGCTGAAGCGAACCGGGAAGAATCGCTGAAAGGATATCCGACAAAAACAGGCTATGACCGTGATGAATGGCCAATGGCT
ATGTGCGAAGAAGGCGGAGCAGGAGCTGACATTCGCTATATTTCACCTTCTGATAACCGCGGAGCCGGCTCATGGGTTGG
AAATCAGTTAGAACAGTATCCAGATGGTACACGCGTACAATTTATCGTTCAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(30-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

66.387

95.968

0.637