Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   V8Z81_RS16660 Genome accession   NZ_CP146257
Coordinates   3252065..3252487 (+) Length   140 a.a.
NCBI ID   WP_013057949.1    Uniprot ID   A0A8D3X0F8
Organism   Priestia megaterium strain MLY91     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3247065..3257487
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V8Z81_RS16635 (V8Z81_16635) - 3247393..3248226 (-) 834 WP_098277670.1 AraC family transcriptional regulator -
  V8Z81_RS16640 (V8Z81_16640) - 3248450..3249271 (+) 822 WP_029322903.1 histidine kinase -
  V8Z81_RS16645 (V8Z81_16645) - 3249272..3249913 (+) 642 WP_057273553.1 response regulator transcription factor -
  V8Z81_RS16650 (V8Z81_16650) - 3250045..3251427 (+) 1383 WP_098288095.1 PepSY domain-containing protein -
  V8Z81_RS16655 (V8Z81_16655) - 3251457..3251924 (+) 468 WP_098526437.1 FixH family protein -
  V8Z81_RS16660 (V8Z81_16660) nucA/comI 3252065..3252487 (+) 423 WP_013057949.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  V8Z81_RS16665 (V8Z81_16665) - 3252641..3252994 (+) 354 WP_223272376.1 DUF1516 family protein -
  V8Z81_RS16670 (V8Z81_16670) - 3253035..3253628 (-) 594 WP_013057951.1 hypothetical protein -
  V8Z81_RS16675 (V8Z81_16675) - 3253784..3254440 (-) 657 WP_013057952.1 TetR/AcrR family transcriptional regulator -
  V8Z81_RS16680 (V8Z81_16680) - 3254464..3255744 (-) 1281 WP_228540830.1 glycosyltransferase -
  V8Z81_RS16685 (V8Z81_16685) - 3256042..3256587 (+) 546 WP_098288097.1 GNAT family N-acetyltransferase -
  V8Z81_RS16690 (V8Z81_16690) - 3256887..3257372 (-) 486 WP_053487488.1 hypothetical protein -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 15329.17 Da        Isoelectric Point: 4.4990

>NTDB_id=942434 V8Z81_RS16660 WP_013057949.1 3252065..3252487(+) (nucA/comI) [Priestia megaterium strain MLY91]
MKKIVSGILILVLAVIVMIEGNPLEQNSSTNTSYDEVITFPADRYPETAAHIQHAIDKGKSAVCTIDRDGAEENRRESLK
GIPTKSGYDRDEFPMAFCEEGGSGADIEYVKPSDNRGAGSWISHQVDEFPDGTKVLIEVK

Nucleotide


Download         Length: 423 bp        

>NTDB_id=942434 V8Z81_RS16660 WP_013057949.1 3252065..3252487(+) (nucA/comI) [Priestia megaterium strain MLY91]
TTGAAAAAAATTGTTTCTGGTATTTTAATTTTAGTATTAGCCGTTATTGTTATGATTGAAGGAAATCCGTTAGAGCAGAA
CTCTTCAACGAATACCTCCTACGATGAAGTAATTACCTTCCCTGCTGATCGTTACCCTGAAACTGCAGCGCACATTCAAC
ATGCAATCGATAAAGGAAAATCGGCTGTTTGTACAATCGACCGAGACGGGGCTGAGGAAAACCGCCGTGAGTCTTTAAAA
GGCATTCCGACTAAAAGCGGCTATGACCGAGATGAGTTTCCGATGGCCTTTTGTGAAGAAGGCGGAAGCGGTGCCGATAT
TGAATATGTAAAGCCTTCAGACAATCGAGGCGCCGGGTCTTGGATCTCACATCAAGTAGACGAGTTTCCTGACGGTACGA
AGGTTTTAATTGAAGTGAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(43-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

61.207

82.857

0.507