Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   V6V88_RS00625 Genome accession   NZ_CP146253
Coordinates   131937..133382 (+) Length   481 a.a.
NCBI ID   WP_056327751.1    Uniprot ID   -
Organism   Achromobacter sp. E1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 126937..138382
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6V88_RS00610 (V6V88_00610) - 127781..128857 (-) 1077 Protein_121 2-isopropylmalate synthase -
  V6V88_RS00615 (V6V88_00615) leuA 129048..130757 (-) 1710 WP_062840150.1 2-isopropylmalate synthase -
  V6V88_RS00620 (V6V88_00620) - 131276..131938 (-) 663 WP_338617247.1 hypothetical protein -
  V6V88_RS00625 (V6V88_00625) rpoN/rpoN1 131937..133382 (+) 1446 WP_056327751.1 RNA polymerase factor sigma-54 Machinery gene
  V6V88_RS00630 (V6V88_00630) - 133504..133782 (+) 279 WP_063954089.1 (2Fe-2S)-binding protein -
  V6V88_RS00635 (V6V88_00635) - 133812..134675 (-) 864 WP_142051936.1 LysR substrate-binding domain-containing protein -
  V6V88_RS00640 (V6V88_00640) - 134809..135720 (+) 912 WP_280004110.1 DMT family transporter -
  V6V88_RS00645 (V6V88_00645) bfr 136113..136589 (+) 477 WP_056327755.1 bacterioferritin -
  V6V88_RS00650 (V6V88_00650) - 136683..137357 (-) 675 WP_062840154.1 hypothetical protein -

Sequence

Protein


Download         Length: 481 a.a.        Molecular weight: 52645.98 Da        Isoelectric Point: 4.7656

>NTDB_id=942352 V6V88_RS00625 WP_056327751.1 131937..133382(+) (rpoN/rpoN1) [Achromobacter sp. E1]
MQNIVPMTRPILELRPGQHLTLTPQLQQSIRLLQLSTLDLEAEISQVLAENPLLDREDEPATAEDRAESERESSVQDDEP
AAERETAIDEMPGSGGVYPDDDASLPEAARPDTLREHLLGQLALTRAAPRDAALASLLIDELDENGYLGSPLDEILGWLP
AEMEPDMDELRAALSLLQSFDPVGIGARDMAECLQLQLRNPDLTLLPEAADPVVLACARRICAEHLPLLATGNAARLCAA
VGCDETTFRAAHSLILRLEPRPGRAWTVPAAEYAVPDVIVRKTRRGWQATLNSAAVPRLQINGLYAQMLGNQKESAHAGL
QAQLQQAKWMIRNVEQRFDTILRVSQAIVERQTAFFSQGPAAMRPLILKDIAGELGLHESTISRATTQKYMLTPFGTLEL
KRFFGTGVSTEGGDATSATAVQTYIRQMVADENRAKPLSDSQIMQKLADQGIVIARRTVAKYREALRIAPAALRKAQASA
R

Nucleotide


Download         Length: 1446 bp        

>NTDB_id=942352 V6V88_RS00625 WP_056327751.1 131937..133382(+) (rpoN/rpoN1) [Achromobacter sp. E1]
ATGCAAAATATTGTACCTATGACCCGTCCAATTTTAGAACTGCGGCCTGGCCAGCACCTGACGCTGACCCCACAACTTCA
GCAATCGATCCGATTGTTGCAGTTGTCCACGCTCGACCTTGAAGCGGAAATCTCGCAAGTGCTGGCCGAGAATCCCCTGC
TGGATCGCGAGGACGAGCCGGCGACGGCGGAGGATCGGGCCGAATCCGAGCGCGAATCTTCGGTTCAGGACGACGAACCG
GCGGCCGAGCGCGAGACCGCCATCGACGAGATGCCTGGCTCGGGCGGGGTGTATCCCGACGACGACGCCAGCCTGCCCGA
GGCGGCGCGGCCGGACACGCTGCGCGAACATCTGCTGGGTCAGCTTGCCCTGACGCGCGCCGCGCCGCGCGATGCCGCGC
TGGCGTCCCTGCTGATCGACGAACTGGACGAAAACGGCTACCTGGGTTCGCCGCTGGATGAAATTCTGGGTTGGCTCCCT
GCCGAAATGGAGCCCGACATGGACGAGCTGCGCGCCGCCCTGTCGCTTCTGCAATCGTTCGACCCGGTGGGCATCGGCGC
GCGCGACATGGCTGAATGCCTGCAACTGCAACTGCGCAATCCCGATCTCACCTTGCTGCCGGAGGCGGCTGACCCCGTCG
TGCTTGCCTGTGCGCGCCGGATCTGCGCCGAGCACCTGCCGCTGTTGGCAACCGGCAACGCCGCGCGTCTGTGTGCGGCG
GTCGGCTGCGACGAGACCACGTTCCGCGCGGCGCATTCGCTGATCCTGCGCCTGGAACCCCGGCCGGGCCGGGCCTGGAC
CGTGCCGGCGGCCGAGTACGCAGTGCCGGACGTCATCGTCCGCAAGACGCGGCGCGGCTGGCAGGCCACGCTGAATAGCG
CCGCGGTGCCTCGGCTGCAGATCAATGGGCTGTACGCCCAGATGCTGGGCAACCAGAAGGAATCCGCCCACGCCGGCCTG
CAGGCCCAGCTGCAGCAGGCCAAATGGATGATCCGCAACGTGGAGCAGCGCTTTGACACCATCCTGCGCGTGTCGCAGGC
CATCGTCGAGCGTCAGACGGCTTTCTTCAGCCAGGGGCCGGCGGCGATGCGTCCGCTCATCCTGAAGGACATCGCCGGTG
AGCTGGGCTTACATGAATCGACCATTTCGCGGGCCACAACACAGAAGTACATGCTCACTCCGTTCGGCACGCTGGAGCTC
AAGCGCTTCTTTGGAACAGGCGTGTCGACAGAAGGCGGCGACGCCACGTCGGCCACCGCGGTGCAGACCTACATCCGCCA
GATGGTGGCCGACGAGAACCGCGCCAAGCCGTTGTCGGATAGCCAGATCATGCAAAAACTGGCCGATCAGGGGATAGTGA
TTGCGCGCCGGACGGTGGCAAAGTACCGTGAAGCGCTGCGGATTGCCCCCGCCGCGCTGCGCAAGGCGCAAGCCTCCGCA
CGCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(108-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(320-475)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(12-55)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

48.907

100

0.511