Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACEZ45_RS16590 Genome accession   NZ_CP169877
Coordinates   3335228..3336538 (-) Length   436 a.a.
NCBI ID   WP_024085826.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain JF     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3330228..3341538
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEZ45_RS16570 (ACEZ45_16565) - 3330472..3331188 (-) 717 WP_007613889.1 (Fe-S)-binding protein -
  ACEZ45_RS16575 (ACEZ45_16570) - 3331368..3332558 (-) 1191 WP_046341431.1 PLP-dependent aminotransferase family protein -
  ACEZ45_RS16580 (ACEZ45_16575) - 3332589..3333308 (-) 720 WP_046341432.1 FadR/GntR family transcriptional regulator -
  ACEZ45_RS16585 (ACEZ45_16580) lutP 3333514..3335190 (+) 1677 WP_003151572.1 L-lactate permease LutP -
  ACEZ45_RS16590 (ACEZ45_16585) rpoN/rpoN1 3335228..3336538 (-) 1311 WP_024085826.1 RNA polymerase factor sigma-54 Machinery gene
  ACEZ45_RS16595 (ACEZ45_16590) yvfG 3336616..3336834 (+) 219 WP_003151570.1 protein YvfG -
  ACEZ45_RS16600 (ACEZ45_16595) - 3336848..3337813 (-) 966 WP_024085827.1 polysaccharide pyruvyl transferase family protein -
  ACEZ45_RS16605 (ACEZ45_16600) - 3337792..3338964 (-) 1173 WP_046341433.1 DegT/DnrJ/EryC1/StrS aminotransferase family protein -
  ACEZ45_RS16610 (ACEZ45_16605) - 3338969..3339616 (-) 648 WP_046341434.1 acetyltransferase -
  ACEZ45_RS16615 (ACEZ45_16610) - 3339613..3340221 (-) 609 WP_032858368.1 sugar transferase -

Sequence

Protein


Download         Length: 436 a.a.        Molecular weight: 49971.97 Da        Isoelectric Point: 9.3840

>NTDB_id=942149 ACEZ45_RS16590 WP_024085826.1 3335228..3336538(-) (rpoN/rpoN1) [Bacillus amyloliquefaciens strain JF]
MDIKLQQTQMLKPQLTQELKQAITLLGYNSAELAGYIDELSLENPLIERKETDTPPLSYHKTNKNRMKERDGSQQIRLTR
KTLQDSLKQQALDMKLSDDEKKIFNYLIHSLDSNGYLKEDIPAAADHVSASQEETEAVLKKLQSLEPAGIGARSLQECIL
LQLRRLPERNEQAEWIAEAYFELFARKNWKALTQKTGIPLKDIQDIADMISALDPRPGLRYAHEEPGFYVEPDIFITVKN
GIIQARLNSRSFPDIQLHAHYQPLLSAAGCRDTKNYLSAKYQEWRWLNRALSQRKQTITRIIGVLTERQESFFLTGKSGM
QPLTLREVAETLELHESTISRAIKGKYVQTPYGLYEMKTFFSAKAESSGDGGASNYAVKAHLEALVRKEDKKKPFSDQKL
ADLLHEQYDIRISRRTVAKYRDQLNIPPSSARKRYS

Nucleotide


Download         Length: 1311 bp        

>NTDB_id=942149 ACEZ45_RS16590 WP_024085826.1 3335228..3336538(-) (rpoN/rpoN1) [Bacillus amyloliquefaciens strain JF]
ATGGATATCAAACTTCAGCAAACACAAATGTTAAAGCCTCAATTAACGCAAGAGCTTAAACAGGCCATCACATTACTCGG
ATACAATTCCGCGGAACTGGCCGGATATATTGATGAGCTCTCACTGGAAAACCCTCTGATTGAACGAAAGGAAACAGACA
CGCCGCCACTATCTTACCATAAAACAAATAAAAACAGGATGAAAGAACGGGACGGAAGCCAGCAGATCAGACTTACCCGC
AAAACGCTGCAGGATTCGCTCAAACAGCAAGCCTTAGACATGAAGCTGTCCGATGATGAGAAAAAAATTTTCAATTATCT
GATACATTCATTGGATTCAAACGGTTATTTAAAAGAGGACATACCAGCCGCCGCCGACCATGTATCCGCTTCACAAGAGG
AGACGGAAGCGGTTCTGAAAAAGCTTCAATCCCTTGAGCCCGCGGGAATCGGCGCCCGGTCTTTGCAGGAATGCATCCTT
CTTCAGCTCCGCCGTCTGCCCGAGCGGAACGAACAGGCGGAATGGATTGCCGAAGCCTATTTTGAGCTATTTGCAAGAAA
GAATTGGAAAGCGCTTACACAGAAAACGGGCATTCCGCTTAAAGACATTCAGGACATCGCTGACATGATCAGCGCGCTTG
ATCCGAGGCCGGGGCTCCGTTATGCGCATGAGGAGCCGGGTTTTTACGTAGAACCGGATATTTTCATCACGGTGAAAAAC
GGCATCATTCAAGCGCGGCTCAACAGCCGTTCGTTTCCTGACATCCAGCTTCATGCCCATTATCAGCCGCTCCTGTCAGC
CGCCGGGTGCCGTGACACGAAAAATTATCTGTCGGCAAAATATCAGGAATGGCGCTGGCTGAACCGGGCTTTGTCGCAAA
GAAAACAAACGATCACGAGAATTATCGGCGTCCTGACTGAACGGCAGGAATCCTTTTTTCTCACAGGAAAAAGCGGAATG
CAGCCGCTGACCCTAAGAGAGGTGGCGGAAACGCTGGAGCTCCACGAATCAACGATCAGCCGGGCCATCAAAGGGAAGTA
CGTTCAGACGCCGTACGGACTGTATGAAATGAAAACATTTTTCTCGGCGAAAGCTGAATCATCGGGGGACGGCGGCGCCT
CCAATTATGCCGTAAAAGCGCATCTGGAAGCATTGGTCCGTAAAGAAGATAAAAAGAAACCTTTTTCAGACCAAAAGCTT
GCCGATCTGCTGCATGAACAATATGACATCCGAATCTCAAGGCGCACCGTCGCCAAATACCGTGACCAATTAAATATTCC
GCCGTCATCGGCGAGAAAACGGTACAGCTGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(275-434)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(77-261)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

32.47

100

0.374