Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   V6657_RS01685 Genome accession   NZ_CP146091
Coordinates   335434..336942 (-) Length   502 a.a.
NCBI ID   WP_048933926.1    Uniprot ID   -
Organism   Ralstonia sp. RRA.1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 330434..341942
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6657_RS01655 - 330900..331553 (-) 654 WP_048933923.1 LON peptidase substrate-binding domain-containing protein -
  V6657_RS01660 rapZ 331652..332554 (-) 903 WP_021196397.1 RNase adapter RapZ -
  V6657_RS01665 - 332648..332992 (-) 345 WP_048933924.1 PsiF family protein -
  V6657_RS01670 hprK 333179..334153 (-) 975 WP_021196399.1 HPr(Ser) kinase/phosphatase -
  V6657_RS01675 ptsN 334227..334682 (-) 456 WP_021196400.1 PTS IIA-like nitrogen regulatory protein PtsN -
  V6657_RS01680 raiA 335029..335382 (-) 354 WP_048933925.1 ribosome-associated translation inhibitor RaiA -
  V6657_RS01685 rpoN/rpoN1 335434..336942 (-) 1509 WP_048933926.1 RNA polymerase factor sigma-54 Machinery gene
  V6657_RS01690 lptB 337074..337862 (-) 789 WP_048933927.1 LPS export ABC transporter ATP-binding protein -
  V6657_RS01695 lptA 337891..338481 (-) 591 WP_048933928.1 lipopolysaccharide transport periplasmic protein LptA -
  V6657_RS01700 lptC 338555..339187 (-) 633 WP_048933929.1 LPS export ABC transporter periplasmic protein LptC -
  V6657_RS01705 - 339192..339779 (-) 588 WP_048933930.1 HAD family hydrolase -
  V6657_RS01710 - 339796..340779 (-) 984 WP_048933931.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 55660.04 Da        Isoelectric Point: 4.8484

>NTDB_id=941654 V6657_RS01685 WP_048933926.1 335434..336942(-) (rpoN/rpoN1) [Ralstonia sp. RRA.1]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVNALQNNNATPLPTE
SPPPSPNGADNSDTADNSYDGHDSSADFDSDYGSDRSDWSLDDFARKPQSDEDDRAPLQLREAEQSLREFLMEQLAPLKL
SMRDKGLVIFLIESLDDEGYLSATLDEILLDLPAELEVEVDELQAALRMLQSFDPPGIGARSAAECLSLQLHRLDSPAKA
LALIIVNQHLELLAARDYTRLKKALSVDEPALKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTSAGWMAQLNPDVM
PRLRINDMYAQILRGSRGEAGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTL
GLHESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVA
RRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=941654 V6657_RS01685 WP_048933926.1 335434..336942(-) (rpoN/rpoN1) [Ralstonia sp. RRA.1]
ATGAAACAGTCGCTCCAGCTCCGTCTCTCTCAGCATCTTGCGCTCACGCCGCAGTTGCAGCAGTCCATCCGGCTGCTGCA
GTTATCGACCATCGAACTGCAGCAGGAAGTTGAACAGGCGCTGACCGAGAACCCGCTGCTGGAGCGGGAAAACGAGTGGC
TCGACACCTCCGCGCGCATCGGCGCGGACGGCTCTGTCAACGCGCTGCAGAACAACAACGCCACGCCGCTGCCCACTGAA
TCGCCGCCGCCCTCGCCCAACGGCGCGGACAATTCCGACACCGCCGACAACAGCTACGACGGCCACGACAGCAGCGCCGA
TTTCGACAGCGATTACGGCTCCGACCGCTCTGACTGGAGCCTCGACGACTTCGCCCGCAAGCCGCAATCCGACGAAGACG
ACCGCGCCCCACTGCAGTTGCGCGAAGCCGAACAAAGCCTGCGCGAATTCCTCATGGAACAGCTCGCGCCGCTCAAGCTG
TCGATGCGCGACAAGGGCCTCGTCATCTTCCTGATCGAATCGCTCGACGATGAGGGCTACCTCTCGGCCACGCTTGACGA
GATCCTGCTCGACCTGCCCGCCGAACTCGAAGTCGAAGTCGATGAGTTGCAGGCCGCGCTGCGCATGCTGCAGAGCTTCG
ATCCGCCCGGCATCGGTGCGCGCTCGGCGGCCGAATGCCTGTCGCTGCAGTTGCATCGGCTCGACTCGCCAGCAAAGGCG
CTCGCGCTCATCATCGTCAACCAGCATCTTGAACTGCTCGCTGCGCGCGACTACACGCGGCTGAAGAAGGCGCTGTCCGT
CGATGAACCCGCGCTGAAGGCAGCGCATGAACTCATTCGTTCGTTGGCGCCGTTCCCCGGCCATGCTTTCGGCCGCGCCG
AAGCCGACTTCGTGGTCCCTGACGTGGTCGTACGCAAAACAAGCGCCGGCTGGATGGCGCAGTTGAACCCGGACGTCATG
CCGCGCCTGCGCATCAACGACATGTACGCGCAGATTCTGCGCGGCAGCCGTGGTGAAGCCGGTGCCGCCAACCTGCAGCA
AAAGCTGCAAGAAGCTCGTTGGCTGATCAAGAACATTCAGCAGCGCTTCGACACGATCCTGCGTGTCTCGCAGGCAATTG
TCGAGCGTCAAAAGAGCTTTTTCACGCACGGTGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTG
GGTCTACACGAGTCCACGATCTCCCGTGTGACGACAAACAAGTACATGGCAACCCCCATGGGGACTTTCGAGCTGAAGTA
CTTCTTCGGCAGCCATGTATCGACGGAAACCGGCGGGGCAGCGTCATCCACTGCGATACGCGCGCTCATCAAGCAACTTG
TAGGAGCCGAAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCC
CGTCGGACGGTGGCCAAATACCGCGAAGCCCTCAAGATCCCTGCAGTGAATCTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(140-329)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-500)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

92.231

100

0.922