Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   V5M36_RS13060 Genome accession   NZ_CP145906
Coordinates   2899779..2900582 (-) Length   267 a.a.
NCBI ID   WP_338559751.1    Uniprot ID   -
Organism   Acinetobacter sp. KS-LM10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2894779..2905582
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V5M36_RS13040 (V5M36_13040) - 2895766..2896509 (-) 744 WP_338559743.1 tetratricopeptide repeat protein -
  V5M36_RS13045 (V5M36_13045) hisS 2896536..2897831 (-) 1296 WP_338559745.1 histidine--tRNA ligase -
  V5M36_RS13050 (V5M36_13050) ispG 2897842..2898960 (-) 1119 WP_338559747.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  V5M36_RS13055 (V5M36_13055) - 2898985..2899788 (-) 804 WP_338559749.1 RodZ domain-containing protein -
  V5M36_RS13060 (V5M36_13060) pilF 2899779..2900582 (-) 804 WP_338559751.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  V5M36_RS13065 (V5M36_13065) rlmN 2900626..2901861 (-) 1236 WP_338559753.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  V5M36_RS13070 (V5M36_13070) ndk 2901983..2902414 (-) 432 WP_338559755.1 nucleoside-diphosphate kinase -
  V5M36_RS13075 (V5M36_13075) iscX 2902523..2902723 (-) 201 WP_338559757.1 Fe-S cluster assembly protein IscX -
  V5M36_RS13080 (V5M36_13080) - 2903111..2903662 (-) 552 WP_338559759.1 phosphatase PAP2 family protein -
  V5M36_RS13085 (V5M36_13085) - 2903682..2904953 (-) 1272 WP_338559760.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 267 a.a.        Molecular weight: 30497.85 Da        Isoelectric Point: 9.7691

>NTDB_id=941071 V5M36_RS13060 WP_338559751.1 2899779..2900582(-) (pilF) [Acinetobacter sp. KS-LM10]
MYKKMVAMTALCLSAWVVQGCQSTGSGTIVKKDPEKSVKVRTQMAAEYIRTGDLDSAKRVLDQALKVDDRDSTSNMMMGV
LLQQEGSQINLDKADAYFKRAISIEPKNAQARNNYGTYLYQVKRYNEAIEQFNIAGSTLGYDQRFKALENLGRIYLLQGD
RVNAEKSFKQALQANRDSSVSMIELAEIFYLQKNFLVARQLYEQFVRIVGQENQGARALWVGLRIARANDDKTGMYVLVS
KLRTQFPNSQEFQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 804 bp        

>NTDB_id=941071 V5M36_RS13060 WP_338559751.1 2899779..2900582(-) (pilF) [Acinetobacter sp. KS-LM10]
ATGTATAAAAAAATGGTTGCTATGACCGCACTGTGTTTATCTGCATGGGTTGTACAAGGGTGTCAGAGCACAGGCTCAGG
CACTATTGTTAAAAAAGATCCAGAAAAATCTGTCAAAGTCCGGACCCAAATGGCTGCTGAGTATATTCGCACTGGTGATT
TGGACTCTGCAAAACGGGTTTTGGATCAAGCGCTTAAAGTCGACGATCGCGATTCGACAAGTAATATGATGATGGGTGTT
TTACTACAACAAGAAGGCAGCCAAATCAATTTAGACAAAGCAGATGCTTATTTTAAGCGTGCAATTTCGATTGAACCTAA
AAATGCACAAGCGCGTAATAATTATGGAACATATTTATATCAAGTAAAACGCTACAATGAGGCGATTGAACAGTTTAATA
TTGCTGGCAGCACGCTGGGTTATGATCAACGTTTTAAAGCGCTCGAAAATCTAGGCCGTATTTACCTGTTACAGGGTGAT
CGGGTGAATGCTGAAAAATCATTCAAACAAGCACTGCAAGCGAACCGTGATTCAAGCGTTTCAATGATAGAGTTAGCTGA
AATTTTTTATCTACAAAAGAATTTCCTTGTAGCACGTCAACTTTATGAACAGTTTGTTCGTATTGTCGGTCAAGAAAATC
AAGGTGCAAGAGCACTTTGGGTTGGTTTACGTATTGCGCGTGCCAATGATGATAAAACGGGAATGTATGTCTTGGTGAGT
AAGCTGAGAACGCAATTCCCTAACAGTCAAGAATTTCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAA
GTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(41-68)

Tetratricopeptide repeat (TPR_17)

(98-130)

Tetratricopeptide repeat (TPR_16)

(150-207)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

72.692

97.378

0.708
  pilF Acinetobacter baylyi ADP1

67.442

96.629

0.652