Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   G6L19_RS00045 Genome accession   NZ_CP169707
Coordinates   7688..9238 (-) Length   516 a.a.
NCBI ID   WP_065703727.1    Uniprot ID   -
Organism   Agrobacterium tumefaciens strain IL30     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2688..14238
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G6L19_RS00015 (G6L19_000015) rdgB 2843..3487 (-) 645 WP_003514751.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  G6L19_RS00020 (G6L19_000020) rph 3505..4221 (-) 717 WP_003514752.1 ribonuclease PH -
  G6L19_RS00025 (G6L19_000025) hrcA 4358..5449 (+) 1092 WP_003514754.1 heat-inducible transcriptional repressor HrcA -
  G6L19_RS00030 (G6L19_000030) grpE 5563..6198 (+) 636 WP_003514756.1 nucleotide exchange factor GrpE -
  G6L19_RS00035 (G6L19_000035) ptsN 6328..6789 (-) 462 WP_003493488.1 PTS IIA-like nitrogen regulatory protein PtsN -
  G6L19_RS00040 (G6L19_000040) hpf 6872..7453 (-) 582 WP_003514759.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  G6L19_RS00045 (G6L19_000045) rpoN/rpoN1 7688..9238 (-) 1551 WP_065703727.1 RNA polymerase factor sigma-54 Machinery gene
  G6L19_RS00050 (G6L19_000050) lptB 9392..10201 (-) 810 WP_003514762.1 LPS export ABC transporter ATP-binding protein -
  G6L19_RS00055 (G6L19_000055) - 10211..10777 (-) 567 WP_003514763.1 LptA/OstA family protein -
  G6L19_RS00060 (G6L19_000060) lptC 10791..11456 (-) 666 WP_038489653.1 LPS export ABC transporter periplasmic protein LptC -
  G6L19_RS00065 (G6L19_000065) sppA 11706..12659 (+) 954 WP_038489656.1 signal peptide peptidase SppA -
  G6L19_RS00070 (G6L19_000070) - 12686..12988 (+) 303 WP_003493505.1 integration host factor subunit beta -
  G6L19_RS00075 (G6L19_000075) - 13053..13394 (+) 342 WP_003514766.1 LapA family protein -

Sequence

Protein


Download         Length: 516 a.a.        Molecular weight: 57083.23 Da        Isoelectric Point: 4.4245

>NTDB_id=939964 G6L19_RS00045 WP_065703727.1 7688..9238(-) (rpoN/rpoN1) [Agrobacterium tumefaciens strain IL30]
MALSASLLLRQNQSLVMTPQLMQSIQLLQMTHFELTQFIAQEVERNPLLEIAANDGDLSSDSPVDAENFGDADSEGSDKA
APVTADSDDWYSDRAANLGEQLDTSFENVFPDDTEPRKPDAPELAGQWKSMPGQESGESYDLDDFVAAKQTLSDHLNQQL
PLAISSVEDRMIADALIGQLDETGYIAVDAVEDVAERLGAAPSAVERILETLQGFDPPGVFSRSLSECLAIQLAQKDRLD
PAMRAFIDNLDLLAKRDFASLKKLCGVDEEDLLDMLAEIRTLNPRPGAGYDSTVSETIVPDIIIRPSSAGGWLVEINPET
LPRVLINQTYFAEVSRHKSREGEDQDFLSECMQTAHWLTRSLDQRARTIMKVATEIVRQQDAFLVNGVDHLRPLNLKTVA
DAIKMHESTVSRVTSKKYMLTPRGLFELKYFFSVSISSVEGGDSHSAEAVRHRIKAMIAQEAADAVLSDDDIVDNLKKSG
IDLARRTVAKYREAMNIPSSVQRRREKKALARLSAF

Nucleotide


Download         Length: 1551 bp        

>NTDB_id=939964 G6L19_RS00045 WP_065703727.1 7688..9238(-) (rpoN/rpoN1) [Agrobacterium tumefaciens strain IL30]
ATGGCATTATCTGCCAGCCTTTTGCTGCGTCAAAACCAGTCTCTCGTGATGACACCGCAACTGATGCAGTCCATCCAGCT
GCTTCAGATGACGCATTTCGAACTGACGCAGTTCATCGCGCAGGAGGTGGAGCGCAATCCTTTGCTTGAAATTGCGGCAA
ACGACGGCGATTTGAGCAGCGATAGCCCTGTTGATGCGGAGAATTTCGGCGATGCCGACAGCGAAGGGTCCGACAAGGCC
GCGCCGGTAACGGCCGACAGCGACGACTGGTACAGCGATCGCGCGGCAAATCTCGGCGAACAGCTCGACACCAGCTTCGA
AAATGTCTTTCCCGACGATACCGAACCCAGAAAGCCCGACGCACCCGAACTTGCGGGGCAGTGGAAATCCATGCCCGGGC
AGGAGTCAGGCGAGAGTTACGATCTCGACGACTTCGTTGCCGCGAAACAGACCCTCAGCGACCATCTCAATCAGCAATTG
CCTCTCGCCATATCCTCTGTGGAAGATAGGATGATTGCCGATGCGCTGATCGGCCAGCTCGACGAGACCGGATATATCGC
CGTAGATGCCGTCGAAGACGTCGCTGAACGGCTGGGGGCGGCGCCTTCGGCAGTAGAGCGCATTCTGGAGACGCTTCAGG
GCTTTGATCCTCCCGGCGTGTTTTCCCGCTCGCTGAGCGAATGTCTGGCGATCCAGCTTGCCCAGAAGGACAGGCTTGAT
CCGGCAATGCGGGCTTTTATCGACAATCTGGATCTTCTGGCGAAACGGGATTTCGCTTCTCTAAAAAAACTCTGCGGCGT
GGATGAGGAAGACCTTCTCGACATGCTGGCGGAAATCCGCACGCTCAATCCGCGTCCGGGCGCTGGTTACGATTCGACCG
TTTCCGAGACAATCGTTCCTGATATCATCATTCGCCCCTCCTCTGCCGGCGGCTGGCTTGTGGAAATCAACCCCGAGACC
CTGCCGCGCGTGCTCATCAACCAGACCTATTTCGCAGAGGTTTCAAGGCACAAGTCGCGCGAGGGAGAGGATCAGGATTT
CCTTTCCGAATGCATGCAGACGGCCCATTGGCTGACCCGCAGCCTCGACCAGCGCGCCAGAACGATCATGAAGGTGGCGA
CCGAAATCGTGCGCCAGCAGGACGCCTTTCTCGTCAATGGCGTGGACCACCTTCGCCCGCTGAACCTCAAGACGGTGGCC
GACGCCATCAAGATGCACGAATCCACCGTCAGCCGGGTGACGTCGAAAAAATACATGCTGACGCCGCGCGGCCTGTTCGA
GCTGAAATATTTCTTCAGTGTTTCCATCAGCTCGGTGGAAGGCGGTGACAGCCATTCGGCAGAAGCGGTGCGTCATCGCA
TCAAGGCGATGATCGCGCAGGAGGCCGCCGACGCAGTGCTTTCCGACGACGATATCGTCGATAATCTGAAAAAGAGTGGT
ATCGATCTTGCCCGCCGGACCGTGGCCAAATATCGCGAGGCGATGAACATCCCATCCTCCGTTCAGAGACGGCGCGAGAA
GAAGGCCCTGGCCAGACTGTCGGCGTTCTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(143-330)

Sigma-54, DNA binding domain (Sigma54_DBD)

(346-505)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(7-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

36.053

100

0.368