Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   V6090_RS00570 Genome accession   NZ_CP145798
Coordinates   121487..122926 (+) Length   479 a.a.
NCBI ID   WP_005336331.1    Uniprot ID   A0A0T6N7F3
Organism   Aeromonas veronii strain CARB25     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 116487..127926
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6090_RS00540 (V6090_00540) mlaF 117064..117864 (-) 801 WP_252412772.1 phospholipid ABC transporter ATP-binding protein MlaF -
  V6090_RS00545 (V6090_00545) - 118072..119067 (+) 996 WP_123172219.1 KpsF/GutQ family sugar-phosphate isomerase -
  V6090_RS00550 (V6090_00550) kdsC 119067..119621 (+) 555 WP_279490111.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  V6090_RS00555 (V6090_00555) lptC 119618..120178 (+) 561 WP_047438732.1 LPS export ABC transporter periplasmic protein LptC -
  V6090_RS00560 (V6090_00560) lptA 120165..120692 (+) 528 WP_040068817.1 lipopolysaccharide transport periplasmic protein LptA -
  V6090_RS00565 (V6090_00565) lptB 120696..121421 (+) 726 WP_005336333.1 LPS export ABC transporter ATP-binding protein -
  V6090_RS00570 (V6090_00570) rpoN/rpoN1 121487..122926 (+) 1440 WP_005336331.1 RNA polymerase factor sigma-54 Machinery gene
  V6090_RS00575 (V6090_00575) hpf 122947..123234 (+) 288 WP_005336323.1 ribosome hibernation promoting factor -
  V6090_RS00580 (V6090_00580) ptsN 123237..123683 (+) 447 WP_005336322.1 PTS IIA-like nitrogen regulatory protein PtsN -
  V6090_RS00585 (V6090_00585) rapZ 123729..124595 (+) 867 WP_021231839.1 RNase adapter RapZ -
  V6090_RS00590 (V6090_00590) npr 124616..124888 (+) 273 WP_005336318.1 PTS phosphocarrier protein NPr -
  V6090_RS00595 (V6090_00595) - 124906..126810 (-) 1905 WP_304520461.1 transcription antiterminator -
  V6090_RS00600 (V6090_00600) dagF 126918..127658 (-) 741 WP_304520462.1 2-dehydro-3-deoxy-phosphogluconate aldolase -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53701.64 Da        Isoelectric Point: 4.3442

>NTDB_id=939875 V6090_RS00570 WP_005336331.1 121487..122926(+) (rpoN/rpoN1) [Aeromonas veronii strain CARB25]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALENNPLLEQEESEAQETSPEAPVDASQLDSSDAMAQEQMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVALED
IQAAVTSDDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLDQYDPQTPWLNEAKEVILEHMELLGNRDYRTLARK
TKLKESDLKEVLDLIQLLEPRPGNGIIQSDSQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNETYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLFIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=939875 V6090_RS00570 WP_005336331.1 121487..122926(+) (rpoN/rpoN1) [Aeromonas veronii strain CARB25]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTTCAGCAGGAAATTCAGCAAGCGCTCGAAAACAACCCTCTGCTGGAGCAGGAAGAGAGCGAGG
CGCAAGAGACCAGTCCGGAAGCTCCGGTCGATGCCAGCCAGCTGGATTCCAGCGATGCCATGGCCCAGGAGCAGATGCCG
GACGAGTTGCCGGTGGATACCACCTGGGACGAGGTTTACTCTGCCGGCACCGCCCAGAGCGCGGGCCCCATCCATGATGG
GGAAGATAGCGTCTATCAGGGTGAAACCACCGAAAACCTGCAGGATTATTTGTTGTGGCAGATGCGACTAACACCGTTCA
GCGATGTGGATGCAGCCATCGCGCTCGCCATCATCGATGCCATCGACGAATCCGGTTATCTGACCGTTGCGCTGGAGGAT
ATCCAGGCTGCCGTTACCAGCGATGACGTAGAGGTGGAACTCGATGAGGTCGAGGCGGTACTTAAGCGGGTTCAGCACTT
CGATCCGGTCGGTATTGCTGCCCGTTCTGTGCAGGAGTGTTTGTTGATCCAGCTGGATCAGTATGACCCGCAGACCCCCT
GGCTCAACGAGGCCAAGGAGGTGATCCTCGAGCACATGGAGCTGCTCGGCAACCGGGATTACCGCACCCTCGCCCGCAAG
ACAAAACTCAAAGAGAGCGACCTCAAGGAGGTGCTGGATTTGATCCAGCTGCTGGAGCCGCGTCCCGGCAACGGCATCAT
CCAGAGCGACTCCCAGTACGTGATCCCCGACGTCTCCGTGGTCAAGAAGGGCAGCAAGTGGGTGGTCGAGCTCAATCCCG
ACTCTGCCCCCCGCATCCGGGTCAACGAGACCTATGCCGCCATGGCCCGCAACGCCCGCAGCAGCACCGACACCCAGTTT
ATTCGCTCCCATTTGCAGGAAGCCAAGTGGTTCATCAAGAGCCTGGAAAGTCGCAATGACACCCTGCTGAAAGTGGCCAG
TTGCATTGTCGAGCAGCAACAGGGCTTTTTCGAGTATGGTGAAGAGGCGATGAAGCCGATGGTGCTCAATGACATCGCCG
AAGCGGTAGAGATGCATGAGTCGACCATCTCCCGGGTGACGACCCAGAAGTACATGCATACCCCCCGTGGTATCTTCGAA
TTGAAGTTTTTTTTCTCCAGCCATGTGAACACCGAAGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTCATCAA
GAAGCTGGTCACGGCAGAGAATCCTGCCAAGCCGTTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCTAAGTATCGCGAATCCTTGTTTATCCCACCTTCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0T6N7F3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.988

100

0.491