Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   V6D45_RS00020 Genome accession   NZ_CP145722
Coordinates   3027..4139 (+) Length   370 a.a.
NCBI ID   WP_014112268.1    Uniprot ID   G4NT00
Organism   Bacillus spizizenii strain kcgeb_S11     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1..9139
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6D45_RS00005 (V6D45_00005) dnaA 1..1341 (+) 1341 WP_003242674.1 chromosomal replication initiator protein DnaA -
  V6D45_RS00010 (V6D45_00010) dnaN 1528..2664 (+) 1137 WP_003219263.1 DNA polymerase III subunit beta -
  V6D45_RS00015 (V6D45_00015) rlbA 2796..3011 (+) 216 WP_003219264.1 ribosome maturation protein RlbA -
  V6D45_RS00020 (V6D45_00020) recF 3027..4139 (+) 1113 WP_014112268.1 DNA replication/repair protein RecF Machinery gene
  V6D45_RS00025 (V6D45_00025) remB 4157..4402 (+) 246 WP_003219266.1 extracellular matrix regulator RemB -
  V6D45_RS00030 (V6D45_00030) gyrB 4457..6373 (+) 1917 WP_014112269.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  V6D45_RS00035 (V6D45_00035) gyrA 6584..9049 (+) 2466 WP_061187096.1 DNA topoisomerase (ATP-hydrolyzing) subunit A -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 42333.38 Da        Isoelectric Point: 7.7139

>NTDB_id=939640 V6D45_RS00020 WP_014112268.1 3027..4139(+) (recF) [Bacillus spizizenii strain kcgeb_S11]
MYIQNLELTSYRNYDHAELQFENKVNVIIGENAQGKTNLMEAIYVLSMAKSHRTSNDKELIRWDKDYAKIEGRVMKQNGA
IPMQLVISKKGKKGKVNHIEQQKLSQYVGALNTIMFAPEDLNLVKGSPQVRRRFLDMEIGQVSPVYLHDLSLYQKILSQR
NHFLKQLQTRKQTDRTMLDVLTDQLVEVAAKVVVKRLQFTAQLEKWAQPIHAGISRGLEELTLKYHTALEVSDPKDLSKI
GDSYQEAFSKLREKEIERGVTLSGPHRDDVLFYVNGRDVQTYGSQGQQRTTALSLKLAEIDLIHEEIGEYPILLLDDVLS
ELDDYRQSHLLHTIQGRVQTFVTTTSVDGIDHETLRQAGMFRVQNGALVK

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=939640 V6D45_RS00020 WP_014112268.1 3027..4139(+) (recF) [Bacillus spizizenii strain kcgeb_S11]
TTGTATATCCAGAACTTAGAACTGACATCTTACCGCAACTACGACCATGCTGAACTTCAATTTGAAAATAAAGTAAATGT
GATCATCGGAGAAAACGCCCAGGGGAAAACAAATCTCATGGAGGCGATCTATGTCTTGTCCATGGCGAAATCGCACAGGA
CGTCAAATGACAAAGAACTTATACGGTGGGACAAAGACTATGCTAAAATAGAGGGAAGAGTGATGAAGCAAAACGGGGCG
ATCCCGATGCAGCTCGTCATCTCCAAAAAGGGTAAAAAGGGCAAGGTCAATCATATTGAACAGCAAAAGCTCAGCCAGTA
TGTCGGGGCCCTCAACACCATTATGTTCGCGCCGGAAGATTTAAATCTTGTAAAGGGAAGCCCTCAAGTGAGAAGGCGGT
TTCTTGACATGGAAATCGGACAGGTTTCTCCTGTTTACCTGCATGACCTTTCTCTTTACCAGAAAATCCTCTCGCAGCGA
AATCATTTTTTGAAGCAGCTGCAAACAAGAAAACAAACTGACAGGACGATGCTCGATGTTTTGACCGATCAGCTTGTAGA
AGTTGCAGCAAAAGTCGTCGTAAAACGCCTGCAATTTACAGCGCAGCTCGAAAAATGGGCTCAGCCTATCCATGCGGGCA
TCTCAAGAGGGCTTGAAGAGCTGACCCTGAAGTACCATACGGCTCTTGAGGTATCAGATCCTAAAGACTTGTCGAAAATA
GGAGATAGCTATCAAGAAGCGTTTTCTAAATTAAGAGAAAAAGAAATTGAGCGCGGTGTTACGCTGTCAGGGCCTCATCG
TGACGATGTTCTTTTCTATGTGAACGGACGCGATGTGCAGACGTATGGTTCTCAAGGACAGCAGCGAACGACGGCATTGT
CTCTTAAGCTGGCGGAGATTGACCTGATCCATGAAGAAATCGGAGAATACCCCATTTTACTATTGGATGATGTACTGAGT
GAACTGGACGATTATCGCCAGTCACACTTGCTTCATACGATCCAAGGCCGTGTACAAACGTTTGTCACAACGACAAGCGT
TGATGGCATTGATCACGAAACCTTACGTCAAGCAGGAATGTTCCGTGTGCAAAATGGTGCGTTAGTGAAGTGA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-350)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G4NT00

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

99.459

100

0.995