Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACE17W_RS10130 Genome accession   NZ_CP169562
Coordinates   2264162..2265556 (-) Length   464 a.a.
NCBI ID   WP_010946226.1    Uniprot ID   Q5ZY95
Organism   Legionella pneumophila strain Lp02     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2259162..2270556
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACE17W_RS10095 (ACE17W_10095) - 2259220..2260230 (-) 1011 WP_010946219.1 class I fructose-bisphosphate aldolase -
  ACE17W_RS10100 (ACE17W_10100) - 2260380..2261114 (-) 735 WP_016356729.1 ferredoxin--NADP reductase -
  ACE17W_RS10105 (ACE17W_10105) dotV 2261198..2261740 (+) 543 WP_010946221.1 type IV secretion system protein DotV -
  ACE17W_RS10110 (ACE17W_10110) - 2261884..2262168 (-) 285 WP_011945638.1 DUF3579 domain-containing protein -
  ACE17W_RS10115 (ACE17W_10115) pssA 2262398..2263135 (+) 738 WP_015444818.1 CDP-diacylglycerol--serine O-phosphatidyltransferase -
  ACE17W_RS10120 (ACE17W_10120) - 2263376..2263645 (-) 270 WP_010946224.1 HPr family phosphocarrier protein -
  ACE17W_RS10125 (ACE17W_10125) hpf 2263836..2264135 (-) 300 WP_010946225.1 ribosome hibernation promoting factor -
  ACE17W_RS10130 (ACE17W_10130) rpoN/rpoN1 2264162..2265556 (-) 1395 WP_010946226.1 RNA polymerase factor sigma-54 Machinery gene
  ACE17W_RS10135 (ACE17W_10135) rpmG 2265766..2265930 (-) 165 WP_003635328.1 50S ribosomal protein L33 -
  ACE17W_RS10140 (ACE17W_10140) rpmB 2265945..2266181 (-) 237 WP_010946227.1 50S ribosomal protein L28 -
  ACE17W_RS10145 (ACE17W_10145) trmB 2266450..2267127 (+) 678 WP_015444815.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  ACE17W_RS10150 (ACE17W_10150) - 2267139..2268263 (+) 1125 WP_015444814.1 M42 family metallopeptidase -
  ACE17W_RS10155 (ACE17W_10155) ankC 2268341..2269828 (+) 1488 WP_010946231.1 Dot/Icm T4SS effector AnkC/LegA12 -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52954.19 Da        Isoelectric Point: 5.2167

>NTDB_id=939503 ACE17W_RS10130 WP_010946226.1 2264162..2265556(-) (rpoN/rpoN1) [Legionella pneumophila strain Lp02]
MKPTLQIGIGQHLTLTPQLQQAIRLLQLSTFDLQQEIQLVVESNPLLEATPVEEKEDAQINTNQNHDEYSDFQWSQLYNN
LNKRNNFEENDYNFDNLHCTTTNLQDHLNWQLDLTPMSDIDRVIATTIIDAINDDGFLTIPVAEIHASLNSDDFPLDIEE
IEAVRHRLQLFDPVGCASVNLAETLVLQLEQLPLEPKLLATAKKVILEDIELLGQHNYRQLMKNHQISEKTIHEILQVIH
KLNPKPGSLIHQDNIEYVIPDLTVKKIDNQWKVFLNQDILPRLSINSQYAALIQRANNSLDNQFLKHNLQEARWFLKSIQ
SRQETLLKVATCIMEYQHGFLEFGEEAMEPLILNDVASALDMHESTVSRVTTQKFINTPRGVFELKYFFSSHVNTDSGGE
CSSTAIRAVIKKLISAENRKKPLSDSKIAQLIGEQGIKVARRTVAKYREAMGIPPSNERKVISS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=939503 ACE17W_RS10130 WP_010946226.1 2264162..2265556(-) (rpoN/rpoN1) [Legionella pneumophila strain Lp02]
ATGAAACCGACATTGCAGATTGGTATTGGTCAACATCTCACGTTAACCCCACAACTGCAACAAGCCATAAGACTGCTGCA
ATTATCCACCTTTGATTTACAACAAGAAATTCAACTCGTTGTTGAATCAAATCCTTTGCTTGAAGCCACTCCTGTTGAAG
AAAAAGAAGACGCCCAAATAAATACTAATCAGAACCATGATGAATATTCAGATTTTCAATGGTCGCAACTGTATAATAAC
TTAAACAAGCGCAATAATTTTGAAGAAAACGATTACAATTTCGATAATTTACACTGCACTACCACCAATTTACAAGATCA
TCTTAACTGGCAACTTGACCTGACGCCTATGAGCGATATTGACCGAGTCATTGCTACAACAATTATTGATGCGATAAACG
ATGACGGCTTTTTAACTATACCCGTTGCTGAAATTCACGCCAGCTTAAATAGCGATGATTTCCCTCTGGATATAGAAGAA
ATAGAAGCAGTGAGACACAGGCTTCAATTATTTGACCCCGTAGGATGCGCATCTGTTAATTTAGCAGAAACACTCGTTTT
GCAATTGGAACAACTTCCACTGGAACCCAAGCTTTTGGCTACTGCCAAGAAAGTCATTCTTGAAGACATTGAGTTGCTAG
GGCAACATAACTACAGACAACTCATGAAAAATCATCAAATAAGTGAAAAAACAATTCATGAAATATTACAAGTCATTCAT
AAATTAAATCCAAAACCAGGAAGTTTGATCCATCAAGATAATATTGAATATGTTATTCCCGACTTGACTGTTAAAAAGAT
AGATAATCAATGGAAAGTTTTTTTGAATCAAGATATTCTTCCCAGGCTGAGTATCAATAGCCAATATGCCGCTTTAATCC
AACGCGCAAACAATAGTCTGGATAATCAATTCTTAAAGCATAATTTGCAAGAAGCACGTTGGTTTTTAAAAAGCATTCAA
AGCCGTCAGGAAACACTTCTAAAAGTAGCAACCTGTATCATGGAATACCAACATGGATTTCTGGAATTTGGTGAAGAAGC
GATGGAACCATTAATATTGAATGATGTAGCTTCTGCCTTGGATATGCATGAATCAACGGTATCAAGAGTTACAACTCAAA
AATTTATTAACACTCCTCGAGGTGTTTTTGAGTTAAAGTACTTCTTCTCCAGCCATGTCAATACTGATAGCGGAGGAGAA
TGCTCTTCCACCGCAATCCGAGCGGTAATAAAAAAATTAATTTCAGCAGAGAATAGAAAAAAACCTTTGAGTGATAGTAA
AATTGCCCAACTAATAGGTGAGCAAGGCATTAAAGTAGCAAGACGTACTGTTGCCAAATATCGCGAAGCAATGGGGATTC
CCCCATCCAATGAAAGAAAGGTTATTAGTAGTTAA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(303-460)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(95-289)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5ZY95

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

39.087

100

0.425