Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   NGK70_RS01510 Genome accession   NZ_AP025730
Coordinates   328944..330572 (-) Length   542 a.a.
NCBI ID   WP_251971623.1    Uniprot ID   -
Organism   Sphaerotilus microaerophilus strain FB-5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 323944..335572
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGK70_RS01485 (CATMQ487_02940) hpaE 324800..326269 (-) 1470 WP_251971618.1 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase -
  NGK70_RS01490 (CATMQ487_02950) - 326283..327062 (-) 780 WP_251971619.1 fumarylacetoacetate hydrolase family protein -
  NGK70_RS01495 (CATMQ487_02960) - 327059..327817 (-) 759 WP_251971620.1 fumarylacetoacetate hydrolase family protein -
  NGK70_RS01500 (CATMQ487_02970) hpaR 327868..328410 (+) 543 WP_251971621.1 homoprotocatechuate degradation operon regulator HpaR -
  NGK70_RS01505 (CATMQ487_02980) - 328471..328785 (-) 315 WP_251971622.1 hypothetical protein -
  NGK70_RS01510 (CATMQ487_02990) rpoN/rpoN1 328944..330572 (-) 1629 WP_251971623.1 RNA polymerase factor sigma-54 Machinery gene
  NGK70_RS01515 (CATMQ487_03000) lptB 330627..331406 (-) 780 WP_251973648.1 LPS export ABC transporter ATP-binding protein -
  NGK70_RS01520 (CATMQ487_03010) lptA 331439..332020 (-) 582 WP_251971624.1 lipopolysaccharide transport periplasmic protein LptA -
  NGK70_RS01525 (CATMQ487_03020) - 332135..332809 (-) 675 WP_251971625.1 thiol:disulfide interchange protein DsbA/DsbL -
  NGK70_RS01530 (CATMQ487_03030) - 332896..333552 (-) 657 WP_251971626.1 SPOR domain-containing protein -
  NGK70_RS01535 (CATMQ487_03040) argS 333606..335291 (-) 1686 WP_251971627.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 542 a.a.        Molecular weight: 59145.47 Da        Isoelectric Point: 4.4398

>NTDB_id=93795 NGK70_RS01510 WP_251971623.1 328944..330572(-) (rpoN/rpoN1) [Sphaerotilus microaerophilus strain FB-5]
MKPSLQVRLSQHLALTPQLQQSIRLLQLSTLELHQEVEQMLEQNPFLEVDDDGSATGDSSAAGATPAEVAAPSAPAEGAA
DTPWDGGSDTLGSEAASPADGDAPGMEPLDFNGGDREDWENGTEREDFDGRAEAPASRNAAPDDEDSDWTERNTVAPSLP
DHLREQLLGMRLAPEDMLLVETLIDSLNEDGYLADALEEVAERLALILELGNPADPAADPQAAAEAALTLREDLLDRLRC
ALGWLQNLEPAGVGARDLCECLQLQLRDLPDSEARDAALAICRGQLEALAKRDLKRLMAITGASEDTVREAQALIVALEP
KPGRRFTRAEANIVVPDVIVQKSGRGWKVVLNPDVMPKLRINDLYAQVLRQHRSQRTGDEGGANLSARLQEARWFVKNIL
QRFDTIQRVSQAIVERQKGFFTHGEIAMKPLVLREIADELGLHESTISRVTTAKYMNTPFGTFELKYFFGSSLNTEAGGN
ASSTAVRALIKQFVSAEDAKKPLSDSQLSQMLEEQGIQVARRTVAKYREALKIAPANLRKAI

Nucleotide


Download         Length: 1629 bp        

>NTDB_id=93795 NGK70_RS01510 WP_251971623.1 328944..330572(-) (rpoN/rpoN1) [Sphaerotilus microaerophilus strain FB-5]
GTGAAACCCTCCCTGCAGGTCCGCCTGTCACAGCACCTGGCGCTGACCCCGCAACTGCAGCAGTCGATCCGGCTGCTGCA
GCTCTCCACGCTGGAGCTGCACCAGGAAGTCGAGCAGATGCTCGAACAGAACCCCTTCCTGGAGGTCGACGACGACGGCA
GCGCCACGGGCGACAGCAGCGCCGCAGGGGCGACGCCGGCCGAGGTGGCGGCGCCGTCCGCGCCGGCCGAAGGAGCCGCC
GACACGCCCTGGGACGGCGGCAGCGACACGCTCGGCAGCGAGGCCGCCAGCCCGGCCGACGGCGATGCCCCGGGCATGGA
GCCGCTGGACTTCAACGGTGGCGACCGTGAAGACTGGGAGAACGGCACCGAGCGCGAGGACTTCGACGGCCGGGCCGAAG
CCCCCGCCAGCCGCAATGCCGCCCCCGACGACGAGGACAGCGACTGGACCGAGCGCAACACCGTGGCCCCCAGCCTGCCC
GACCACCTGCGCGAACAGCTGCTGGGCATGCGCCTGGCGCCCGAGGACATGCTGCTGGTCGAGACGCTGATCGACTCGCT
CAACGAGGACGGCTACCTGGCCGACGCGCTCGAAGAAGTGGCCGAGCGTCTGGCGCTGATCCTGGAGCTGGGCAACCCGG
CCGACCCTGCGGCCGATCCCCAGGCGGCGGCCGAGGCAGCCCTGACGCTGCGCGAGGACCTGCTCGACCGCCTGCGCTGC
GCGCTGGGCTGGCTGCAGAACCTGGAGCCGGCGGGTGTGGGCGCACGCGACCTCTGCGAGTGCCTGCAGCTGCAGCTGCG
TGACCTGCCCGATTCCGAGGCGCGCGACGCCGCCCTGGCGATCTGTCGCGGCCAGCTCGAAGCGCTGGCCAAGCGGGACC
TCAAGCGCCTGATGGCCATCACCGGCGCCAGTGAAGACACCGTGCGCGAGGCGCAGGCGCTGATCGTTGCGCTGGAGCCC
AAGCCGGGGCGGCGCTTCACCCGCGCCGAGGCCAACATCGTCGTGCCCGACGTGATCGTGCAGAAGTCCGGCCGCGGCTG
GAAGGTGGTGCTCAACCCGGACGTGATGCCCAAGCTGCGCATCAATGACCTCTACGCCCAGGTGCTGCGCCAGCACCGCA
GCCAGCGCACCGGCGACGAGGGTGGCGCCAACCTGTCGGCCCGGCTTCAGGAGGCGCGCTGGTTCGTCAAGAACATCCTG
CAGCGCTTCGACACCATCCAGCGCGTCTCGCAGGCCATCGTCGAACGGCAGAAGGGCTTCTTCACCCACGGCGAGATCGC
GATGAAGCCGCTGGTGCTGCGCGAGATCGCCGACGAGCTGGGCCTGCACGAGTCCACCATCTCGCGGGTGACGACGGCCA
AGTACATGAACACGCCCTTCGGCACCTTCGAGCTGAAGTACTTCTTCGGCTCCTCGCTGAACACCGAGGCCGGTGGCAAC
GCCAGCTCCACGGCGGTGCGTGCGCTGATCAAGCAGTTCGTCAGCGCCGAGGACGCCAAGAAGCCGCTGTCGGACAGCCA
GCTCAGCCAGATGCTGGAGGAACAGGGCATCCAGGTGGCGCGGCGCACGGTGGCCAAGTACCGCGAGGCGCTCAAGATCG
CGCCCGCGAACCTGCGCAAGGCGATCTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(385-540)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(152-365)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-49)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.065

100

0.535

Multiple sequence alignment