Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACET1J_RS02575 Genome accession   NZ_CP168998
Coordinates   530581..532014 (-) Length   477 a.a.
NCBI ID   WP_347938119.1    Uniprot ID   -
Organism   Escherichia coli strain UTAK-14     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 525581..537014
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACET1J_RS02540 (ACET1J_02540) elbB 526272..526925 (+) 654 WP_001299134.1 isoprenoid biosynthesis glyoxalase ElbB -
  ACET1J_RS02545 (ACET1J_02545) mtgA 526922..527650 (+) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ACET1J_RS02550 (ACET1J_02550) yrbL 527647..528279 (-) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  ACET1J_RS02555 (ACET1J_02555) npr 528493..528765 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  ACET1J_RS02560 (ACET1J_02560) rapZ 528762..529616 (-) 855 WP_000243741.1 RNase adapter RapZ -
  ACET1J_RS02565 (ACET1J_02565) ptsN 529662..530153 (-) 492 WP_001299132.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACET1J_RS02570 (ACET1J_02570) hpf 530271..530558 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  ACET1J_RS02575 (ACET1J_02575) rpoN/rpoN1 530581..532014 (-) 1434 WP_347938119.1 RNA polymerase factor sigma-54 Machinery gene
  ACET1J_RS02580 (ACET1J_02580) lptB 532062..532787 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  ACET1J_RS02585 (ACET1J_02585) lptA 532794..533351 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ACET1J_RS02590 (ACET1J_02590) lptC 533320..533895 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  ACET1J_RS02595 (ACET1J_02595) kdsC 533892..534458 (-) 567 WP_000030016.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACET1J_RS02600 (ACET1J_02600) kdsD 534479..535465 (-) 987 WP_001368728.1 arabinose-5-phosphate isomerase KdsD -
  ACET1J_RS02605 (ACET1J_02605) yrbG 535479..536456 (-) 978 WP_000922872.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54079.88 Da        Isoelectric Point: 4.3447

>NTDB_id=937733 ACET1J_RS02575 WP_347938119.1 530581..532014(-) (rpoN/rpoN1) [Escherichia coli strain UTAK-14]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDDDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEVSSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=937733 ACET1J_RS02575 WP_347938119.1 530581..532014(-) (rpoN/rpoN1) [Escherichia coli strain UTAK-14]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTAGCGGCAAAAGATCTGCGTGACTGCCTGCTAATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGATGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGTTTCCTCCACGGCGATTCGTGCACTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTACTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.024

100

0.463