Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACET1C_RS02565 Genome accession   NZ_CP168987
Coordinates   530579..532012 (-) Length   477 a.a.
NCBI ID   WP_373929583.1    Uniprot ID   -
Organism   Escherichia coli strain UTAK-10     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 525579..537012
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACET1C_RS02530 (ACET1C_02530) elbB 526270..526923 (+) 654 WP_001299134.1 isoprenoid biosynthesis glyoxalase ElbB -
  ACET1C_RS02535 (ACET1C_02535) mtgA 526920..527648 (+) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ACET1C_RS02540 (ACET1C_02540) yrbL 527645..528277 (-) 633 WP_000620405.1 PhoP regulatory network protein YrbL -
  ACET1C_RS02545 (ACET1C_02545) npr 528491..528763 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  ACET1C_RS02550 (ACET1C_02550) rapZ 528760..529614 (-) 855 WP_000243741.1 RNase adapter RapZ -
  ACET1C_RS02555 (ACET1C_02555) ptsN 529660..530151 (-) 492 WP_001299132.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACET1C_RS02560 (ACET1C_02560) hpf 530269..530556 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  ACET1C_RS02565 (ACET1C_02565) rpoN/rpoN1 530579..532012 (-) 1434 WP_373929583.1 RNA polymerase factor sigma-54 Machinery gene
  ACET1C_RS02570 (ACET1C_02570) lptB 532060..532785 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  ACET1C_RS02575 (ACET1C_02575) lptA 532792..533349 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ACET1C_RS02580 (ACET1C_02580) lptC 533318..533893 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  ACET1C_RS02585 (ACET1C_02585) kdsC 533890..534456 (-) 567 WP_000030016.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACET1C_RS02590 (ACET1C_02590) kdsD 534477..535463 (-) 987 WP_001368728.1 arabinose-5-phosphate isomerase KdsD -
  ACET1C_RS02595 (ACET1C_02595) yrbG 535477..536454 (-) 978 WP_000922872.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54051.87 Da        Isoelectric Point: 4.3447

>NTDB_id=937492 ACET1C_RS02565 WP_373929583.1 530579..532012(-) (rpoN/rpoN1) [Escherichia coli strain UTAK-10]
MKQGLQLKLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDDDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEVSSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=937492 ACET1C_RS02565 WP_373929583.1 530579..532012(-) (rpoN/rpoN1) [Escherichia coli strain UTAK-10]
ATGAAGCAAGGTTTGCAACTCAAGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTAGCGGCAAAAGATCTGCGTGACTGCCTGCTAATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGATGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGTTTCCTCCACGGCGATTCGTGCACTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTACTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

43.825

100

0.461