Detailed information NTG0093748

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LLID5_RS09250
• Genome accession: NZ_AP025701
• Coordinates: 1932733..1933380 (-)
• Length: 215 a.a.
• NCBI ID: WP_058203544.1
• Uniprot ID: A0AB35KBC8
• Organism: Lactococcus lactis strain ID-5
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1927733..1938380

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLID5_RS09225 (LLID5_18220)	atpF	1927802..1928308 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
LLID5_RS09230 (LLID5_18230)	atpB	1928323..1929036 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
LLID5_RS09235 (LLID5_18240)	-	1929081..1929296 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
LLID5_RS09240 (LLID5_18250)	-	1929481..1930257 (-)	777	WP_004255244.1	alpha/beta hydrolase family protein	-
LLID5_RS09245 (LLID5_18260)	comEC	1930542..1932752 (-)	2211	WP_251918536.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LLID5_RS09250 (LLID5_18270)	comEA	1932733..1933380 (-)	648	WP_058203544.1	ComEA family DNA-binding protein	Machinery gene
LLID5_RS09255 (LLID5_18280)	-	1933440..1934801 (-)	1362	WP_029344292.1	ABC transporter permease	-
LLID5_RS09260 (LLID5_18290)	-	1934798..1935730 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
LLID5_RS09265 (LLID5_18300)	-	1935834..1936232 (-)	399	WP_057720189.1	hypothetical protein	-
LLID5_RS09270 (LLID5_18310)	-	1936336..1936899 (-)	564	WP_251918539.1	GNAT family N-acetyltransferase	-
LLID5_RS09275 (LLID5_18320)	-	1937072..1938250 (-)	1179	WP_012898345.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23634.80 Da
• Isoelectric Point: 4.8659

>NTDB_id=93748 LLID5_RS09250 WP_058203544.1 1932733..1933380(-) (comEA) [Lactococcus lactis strain ID-5]
MDKILEKVKEYWKMIVLIVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISPDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
VTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=93748 LLID5_RS09250 WP_058203544.1 1932733..1933380(-) (comEA) [Lactococcus lactis strain ID-5]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAATTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGCCAGATGAACGTCTAGTTGATCTAATTAAGGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
GTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATTGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (151-212)

• SLBB domain (SLBB) (70-125)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	74.771	100	0.758
comEA/celA/cilE	Streptococcus mitis SK321	44.444	100	0.447
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.119	100	0.437
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.096	100	0.419
comEA/celA/cilE	Streptococcus pneumoniae D39	41.096	100	0.419
comEA/celA/cilE	Streptococcus pneumoniae R6	41.096	100	0.419
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.284	100	0.419
comEA	Latilactobacillus sakei subsp. sakei 23K	36.123	100	0.381