Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   V5F86_RS13105 Genome accession   NZ_CP145137
Coordinates   2575748..2576158 (-) Length   136 a.a.
NCBI ID   WP_014114486.1    Uniprot ID   -
Organism   Bacillus spizizenii strain kcgeb_sc     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2570748..2581158
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V5F86_RS13075 (V5F86_13080) - 2570766..2570906 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  V5F86_RS13080 (V5F86_13085) - 2571207..2571938 (-) 732 WP_338442869.1 SGNH/GDSL hydrolase family protein -
  V5F86_RS13085 (V5F86_13090) - 2572189..2572941 (-) 753 WP_338442870.1 N-acetylmuramoyl-L-alanine amidase -
  V5F86_RS13090 (V5F86_13095) - 2573135..2573761 (+) 627 WP_268393378.1 TVP38/TMEM64 family protein -
  V5F86_RS13095 (V5F86_13100) gnd 2573780..2574673 (-) 894 WP_338442871.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  V5F86_RS13100 (V5F86_13105) - 2574999..2575715 (+) 717 WP_338442872.1 DUF308 domain-containing protein -
  V5F86_RS13105 (V5F86_13110) nucA/comI 2575748..2576158 (-) 411 WP_014114486.1 sporulation-specific Dnase NucB Machinery gene
  V5F86_RS13110 (V5F86_13115) sigK 2576354..2577082 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  V5F86_RS13115 (V5F86_13120) - 2577205..2577402 (-) 198 Protein_2534 recombinase family protein -
  V5F86_RS13120 (V5F86_13125) - 2577360..2577538 (-) 179 Protein_2535 hypothetical protein -
  V5F86_RS13125 (V5F86_13130) - 2578221..2579444 (-) 1224 WP_166850462.1 MFS transporter -
  V5F86_RS13130 (V5F86_13135) - 2579921..2580406 (-) 486 WP_010330742.1 DedA family protein -
  V5F86_RS13135 (V5F86_13140) pssA 2580393..2580929 (-) 537 WP_338442873.1 CDP-diacylglycerol--serine O-phosphatidyltransferase -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14938.98 Da        Isoelectric Point: 5.1643

>NTDB_id=937429 V5F86_RS13105 WP_014114486.1 2575748..2576158(-) (nucA/comI) [Bacillus spizizenii strain kcgeb_sc]
MKKWMAGLFLAAAVLLCLMVPQQIQGASLYDKVLYFPLSRYPETGDHIKDAIADGHSDICTIDRDGADKRRQESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSGYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=937429 V5F86_RS13105 WP_014114486.1 2575748..2576158(-) (nucA/comI) [Bacillus spizizenii strain kcgeb_sc]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
TTCTTTGTATGACAAAGTGTTGTATTTTCCGCTGTCTCGTTATCCGGAAACGGGGGATCATATTAAAGATGCGATTGCAG
ATGGTCATTCTGATATTTGCACCATCGACAGGGATGGAGCGGACAAAAGGCGGCAGGAATCATTAAAAGGAATTCCGACG
AAGCCGGGCTATGATCGGGATGAGTGGCCGATGGCGGTTTGTGAGGAAGGCGGCGCAGGAGCTGATGTCCGATATGTCAC
GCCTTCTGATAATCGCGGGGCCGGCTCGTGGGTAGGGAATCAAATGAGCGGTTATCCTGACGGCACCAGAGTGCTCTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529