Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   V5F86_RS12855 Genome accession   NZ_CP145137
Coordinates   2532241..2533008 (-) Length   255 a.a.
NCBI ID   WP_014114447.1    Uniprot ID   -
Organism   Bacillus spizizenii strain kcgeb_sc     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2527241..2538008
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V5F86_RS12835 (V5F86_12840) - 2527415..2528227 (-) 813 WP_079287971.1 pyruvate, water dikinase regulatory protein -
  V5F86_RS12840 (V5F86_12845) ccpN 2528253..2528891 (-) 639 WP_003237058.1 transcriptional regulator CcpN -
  V5F86_RS12845 (V5F86_12850) glyS 2529024..2531063 (-) 2040 WP_338442853.1 glycine--tRNA ligase subunit beta -
  V5F86_RS12850 (V5F86_12855) glyQ 2531056..2531943 (-) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  V5F86_RS12855 (V5F86_12860) recO 2532241..2533008 (-) 768 WP_014114447.1 DNA repair protein RecO Machinery gene
  V5F86_RS12860 (V5F86_12865) - 2533045..2533188 (-) 144 WP_003230053.1 YqzL family protein -
  V5F86_RS12865 (V5F86_12870) era 2533332..2534237 (-) 906 WP_338442854.1 GTPase Era -
  V5F86_RS12870 (V5F86_12875) - 2534218..2534628 (-) 411 WP_003226204.1 cytidine deaminase -
  V5F86_RS12875 (V5F86_12880) - 2534749..2535120 (-) 372 WP_072566561.1 diacylglycerol kinase family protein -
  V5F86_RS12880 (V5F86_12885) ybeY 2535101..2535574 (-) 474 WP_338442855.1 rRNA maturation RNase YbeY -
  V5F86_RS12885 (V5F86_12890) pgpH 2535575..2537710 (-) 2136 WP_072566562.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29331.06 Da        Isoelectric Point: 8.5539

>NTDB_id=937425 V5F86_RS12855 WP_014114447.1 2532241..2533008(-) (recO) [Bacillus spizizenii strain kcgeb_sc]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTTGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDKGTEEKKPNPYLFEFILESFKQLNEGTDPDVIIFIAQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRVPIKPQTARLLRLFYYFDLSRLGNVSLKQETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=937425 V5F86_RS12855 WP_014114447.1 2532241..2533008(-) (recO) [Bacillus spizizenii strain kcgeb_sc]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTTACATTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCAAGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCATTTTTAATGCAAAAAACAACGGGCCTCGGAACGCTTCAGCAGGGTGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGATTTGTTTTTAACAGCATATGCTGCTTATATTGCTGAGCTAGTCGACAAAGGCACAGAAGAAAAGAAGCC
AAACCCTTATTTGTTTGAATTCATTTTAGAATCGTTTAAACAGCTGAATGAAGGAACAGATCCGGATGTCATCATCTTCA
TTGCTCAAATGAAGATGCTCGGTGTCATGGGGCTGTACCCTGAGCTGAATCACTGTGTGCATTGTAAAAGCCAGGATGGG
ACCTTTCATTTTTCTGTTCGTGATAACGGTTTTATTTGCCATCGCTGCTTTGAAAAAGATCCCTACAGGGTTCCAATTAA
ACCGCAAACGGCAAGGCTTTTGAGACTGTTTTATTACTTCGATTTGTCGAGGCTGGGCAATGTGTCCTTAAAGCAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTATGAAGAATATTCAGGGTTATATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTGATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-75)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

96.863

100

0.969