Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   V5F86_RS01965 Genome accession   NZ_CP145137
Coordinates   367228..367677 (-) Length   149 a.a.
NCBI ID   WP_120698809.1    Uniprot ID   -
Organism   Bacillus spizizenii strain kcgeb_sc     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 362228..372677
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V5F86_RS01925 (V5F86_01925) - 362641..362777 (+) 137 Protein_335 hypothetical protein -
  V5F86_RS01930 (V5F86_01930) - 362774..363229 (+) 456 WP_003224877.1 RDD family protein -
  V5F86_RS01935 (V5F86_01935) - 363312..363644 (+) 333 WP_014112608.1 YckD family protein -
  V5F86_RS01940 (V5F86_01940) - 363779..364201 (+) 423 WP_088680288.1 MarR family transcriptional regulator -
  V5F86_RS01945 (V5F86_01945) - 364189..364517 (+) 329 Protein_339 DUF3147 family protein -
  V5F86_RS01950 (V5F86_01950) - 364537..365265 (+) 729 WP_268391405.1 AAA family ATPase -
  V5F86_RS01955 (V5F86_01955) bglC 365338..366771 (+) 1434 WP_090557351.1 6-phospho-beta-glucosidase -
  V5F86_RS01960 (V5F86_01960) nin/comJ 366807..367205 (-) 399 WP_014112613.1 DNA-entry nuclease inhibitor Regulator
  V5F86_RS01965 (V5F86_01965) nucA/comI 367228..367677 (-) 450 WP_120698809.1 DNA-entry nuclease Machinery gene
  V5F86_RS01970 (V5F86_01970) tlpC 367851..369575 (-) 1725 WP_338443736.1 methyl-accepting chemotaxis protein TlpC -
  V5F86_RS01975 (V5F86_01975) hxlB 369687..370244 (-) 558 WP_338443737.1 6-phospho-3-hexuloisomerase -
  V5F86_RS01980 (V5F86_01980) hxlA 370249..370881 (-) 633 WP_338443738.1 3-hexulose-6-phosphate synthase -
  V5F86_RS01985 (V5F86_01985) hxlR 371113..371475 (+) 363 WP_014112618.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16391.38 Da        Isoelectric Point: 4.4321

>NTDB_id=937384 V5F86_RS01965 WP_120698809.1 367228..367677(-) (nucA/comI) [Bacillus spizizenii strain kcgeb_sc]
MNITMDIIKTILLVIVVIAAAAVGLIKGDFFSADQKTSQTKEYDETIALPSDRYPETAKHIKDAINEGHSDVCTIDRDGA
EERREQSLKDVPPKTGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=937384 V5F86_RS01965 WP_120698809.1 367228..367677(-) (nucA/comI) [Bacillus spizizenii strain kcgeb_sc]
GTGAACATCACGATGGACATCATAAAAACAATACTTCTCGTCATCGTCGTCATAGCAGCTGCAGCTGTCGGCCTGATAAA
GGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGACGAAACGATCGCCCTCCCTTCTGACCGTT
ATCCCGAAACGGCCAAACATATTAAGGATGCGATAAATGAGGGGCACTCAGATGTGTGCACCATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAAGACGTACCGCCAAAAACGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCTGCTGACAACCGCGGTGCAGGCTCTTGGGTTGGGCATC
AGCTTACCGATTATCCAGATGGCACAAAGGTTTTATTCACGATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(53-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

94.631

100

0.946