Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AAX72_RS07825 Genome accession   NZ_CP145109
Coordinates   1560101..1561477 (+) Length   458 a.a.
NCBI ID   WP_024525645.1    Uniprot ID   A0A1X0XL19
Organism   Levilactobacillus brevis strain 47f     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1555101..1566477
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAX72_RS07800 (AAX72_007800) - 1555133..1556365 (+) 1233 WP_047021152.1 serine hydrolase domain-containing protein -
  AAX72_RS07805 (AAX72_007805) - 1556499..1557833 (-) 1335 WP_047021153.1 C1 family peptidase -
  AAX72_RS07810 (AAX72_007810) rpiA 1558253..1558939 (-) 687 WP_011667345.1 ribose-5-phosphate isomerase RpiA -
  AAX72_RS07815 (AAX72_007815) - 1558947..1559288 (-) 342 WP_042521562.1 hypothetical protein -
  AAX72_RS07820 (AAX72_007820) - 1559467..1560006 (+) 540 WP_011667347.1 dUTP diphosphatase -
  AAX72_RS07825 (AAX72_007825) radA 1560101..1561477 (+) 1377 WP_024525645.1 DNA repair protein RadA Machinery gene
  AAX72_RS07830 (AAX72_007830) - 1561513..1562697 (+) 1185 WP_021741476.1 PIN/TRAM domain-containing protein -
  AAX72_RS07835 (AAX72_007835) gltX 1562954..1564444 (+) 1491 WP_011667350.1 glutamate--tRNA ligase -
  AAX72_RS07840 (AAX72_007840) cysS 1564599..1566011 (+) 1413 WP_011667351.1 cysteine--tRNA ligase -
  AAX72_RS07845 (AAX72_007845) - 1566011..1566418 (+) 408 WP_011667352.1 Mini-ribonuclease 3 -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49788.09 Da        Isoelectric Point: 7.0881

>NTDB_id=937222 AAX72_RS07825 WP_024525645.1 1560101..1561477(+) (radA) [Levilactobacillus brevis strain 47f]
MAKPRTTYVCQNCDYSSPRYLGRCPNCGEWNTMVEEVVTPAAAKPQSTRTTASGERSHPQLMSEIKHSTESRTKTQMEEL
NRVLGGGIVPGSLILIGGDPGIGKSTLLLQVSGQLSQTGGKVLYVSGEESASQIKMRADRLVVNSDHLYLYPETDMASIR
ANIEQMKPDYVVIDSVQTMQAPGIESAIGSVSQIRAVTGELMQIAKTNGITIFVVGHVTKGGAIAGPKILEHMVDTVLYF
EGDLHHTYRILRAVKNRFGSTNELGIFEMREGGLYEVANPSEIFLEERLKDATGSAIVVSMEGTRPILVEVQALITPSVF
GNAQRTSSGLDRNRVSLLMAVLEKRANLMLQNQDAFLKAAGGVKLDEPAIDLAIALSIASSYRDTATQPTDCFVGEVGLT
GEIRRVNRIEQRVAEAKKLGFKRIFIPKNNLQGWTPDPGIDVVGVSTLRQTLKLALDV

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=937222 AAX72_RS07825 WP_024525645.1 1560101..1561477(+) (radA) [Levilactobacillus brevis strain 47f]
GTGGCGAAACCAAGAACAACGTATGTGTGTCAAAATTGTGATTATAGCTCACCCCGTTATTTGGGGCGTTGTCCCAATTG
TGGTGAGTGGAACACGATGGTGGAAGAAGTCGTCACACCGGCTGCCGCCAAGCCACAATCAACCCGGACCACTGCTTCTG
GAGAACGGTCACACCCCCAACTCATGAGTGAGATTAAACACTCAACGGAAAGCCGAACGAAGACGCAAATGGAAGAACTG
AACCGAGTGCTGGGTGGGGGGATTGTGCCGGGATCTTTGATCCTAATCGGTGGTGATCCTGGAATTGGAAAATCAACCCT
CTTGCTTCAAGTATCGGGGCAACTCAGCCAAACAGGCGGTAAAGTGTTGTATGTTTCTGGAGAAGAAAGTGCGTCACAGA
TTAAAATGCGGGCGGATCGGTTAGTCGTCAATAGCGACCACTTGTATCTTTATCCAGAAACTGATATGGCCAGTATTCGC
GCGAACATTGAACAGATGAAACCTGATTACGTGGTCATTGATTCCGTTCAGACCATGCAGGCGCCGGGAATCGAGTCGGC
GATTGGCTCGGTCTCACAAATTCGTGCGGTCACGGGTGAGCTGATGCAAATTGCCAAAACCAATGGCATCACTATTTTTG
TGGTGGGGCATGTGACGAAGGGCGGCGCAATTGCCGGTCCTAAGATTCTGGAACACATGGTGGATACGGTTCTTTACTTT
GAAGGTGACCTCCATCATACGTACCGAATTCTCCGAGCGGTCAAGAACCGATTCGGGTCAACGAATGAATTGGGTATCTT
TGAAATGCGTGAAGGTGGCTTGTATGAAGTTGCTAATCCGTCAGAAATTTTCTTGGAAGAACGATTGAAGGACGCAACCG
GCTCGGCCATTGTGGTATCAATGGAAGGTACGCGGCCAATTTTGGTAGAAGTTCAGGCGTTAATTACACCATCGGTCTTT
GGTAATGCGCAACGCACATCCAGCGGCCTGGATCGTAATCGGGTATCGTTGTTGATGGCTGTGCTAGAAAAACGGGCCAA
TCTAATGCTCCAAAATCAAGATGCATTCTTAAAGGCAGCAGGTGGCGTTAAGCTGGATGAACCGGCGATTGATTTAGCGA
TTGCACTGTCGATTGCCTCAAGTTATCGTGATACGGCCACGCAGCCAACGGATTGCTTTGTGGGTGAAGTTGGCCTGACT
GGTGAAATCCGTCGGGTTAATCGAATTGAACAACGGGTTGCGGAAGCAAAAAAGCTGGGCTTTAAGCGGATCTTCATTCC
CAAGAATAACCTTCAAGGGTGGACGCCAGATCCCGGAATTGATGTGGTCGGCGTGTCAACGTTACGACAGACGTTAAAAC
TGGCCTTGGACGTGTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(64-222)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1X0XL19

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

66.667

98.908

0.659
  radA Streptococcus mitis NCTC 12261

66.446

98.908

0.657
  radA Streptococcus pneumoniae Rx1

66.446

98.908

0.657
  radA Streptococcus pneumoniae D39

66.446

98.908

0.657
  radA Streptococcus pneumoniae R6

66.446

98.908

0.657
  radA Streptococcus pneumoniae TIGR4

66.446

98.908

0.657
  radA Bacillus subtilis subsp. subtilis str. 168

64.035

99.563

0.638