Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   ACEPM2_RS17415 Genome accession   NZ_CP168735
Coordinates   3661295..3663601 (-) Length   768 a.a.
NCBI ID   WP_011003186.1    Uniprot ID   Q8XU91
Organism   Ralstonia pseudosolanacearum strain RUN3223     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3656295..3668601
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEPM2_RS17410 (ACEPM2_17410) putA 3657156..3661133 (-) 3978 WP_011003185.1 trifunctional transcriptional regulator/proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase -
  ACEPM2_RS17415 (ACEPM2_17415) priA 3661295..3663601 (-) 2307 WP_011003186.1 primosomal protein N' Machinery gene
  ACEPM2_RS17420 (ACEPM2_17420) hemE 3664184..3665278 (-) 1095 WP_011003187.1 uroporphyrinogen decarboxylase -
  ACEPM2_RS17425 (ACEPM2_17425) - 3665420..3665887 (-) 468 WP_011003188.1 cupin domain-containing protein -
  ACEPM2_RS17430 (ACEPM2_17430) - 3666052..3666399 (+) 348 WP_011003189.1 GFA family protein -
  ACEPM2_RS17435 (ACEPM2_17435) - 3666428..3666808 (+) 381 WP_011003190.1 hypothetical protein -

Sequence

Protein


Download         Length: 768 a.a.        Molecular weight: 82844.30 Da        Isoelectric Point: 8.3490

>NTDB_id=936865 ACEPM2_RS17415 WP_011003186.1 3661295..3663601(-) (priA) [Ralstonia pseudosolanacearum strain RUN3223]
MTATRVDAAPPAVAQAARAGAIARVVIDTPLDAVFDYRCAQPVVPGQLVVVPFGSRRVVALVVESAGTTEVPADRLRDVE
RVLDWVPPLDAEWRALAEFAAGYYHRALGEVTLPALPPLWRTPGSWDSLARHATETVYVMREPARAALLDSIPRRAAGAM
RLAEALTGDGELSASAAVALHGQATAKLRDWAAVGWVGAELRPRPLLPVPRDLPPASVAPALNAEQAAAVEAIAQAGAGG
GFAPFLLYGVTGSGKTEVYLHAIADALARDPAAQVLMLVPEINLTPQLEARIAARFPGVPLAALHSGLAEQPRALNWLAA
HRGEARIVLGTRLAMLASLPHLALILVDEEHDTSYKQQEGLRYSARDLALWRAKQRGIPVVLGSATPSLETWWRAEQGTT
ARLVLSQRAQADAVLPRVSLIDLTLERRAGREIRDGLSRPLLDAVAGRLARGEQSLLFLNRRGYAPVLACDACGWLSGCP
RCSAYLVLHKPEHRLRCHHCGYESRIPHHCPDCGNVDIAPLGRGTQRIEETLGALFPQARVARIDADSTRRKGSAEALFD
TVHEGAVDILIGTQMVAKGHDFQRVTLVGVVAPDASLFSHDFRAGEHLFASLMQVAGRAGRAGLAGEVLIQTRYPDAPAL
LALVRHDYAGFARQLLRERKQACLPPFAYQALLTAEHRELARALEFLGAARAAGQAVAEAQSLPVQLHDPVPMTMVRLAN
RERAQLLLESASRAALQRLLATWTDGFGDIGRTVKGVRWQLEIDPLRI

Nucleotide


Download         Length: 2307 bp        

>NTDB_id=936865 ACEPM2_RS17415 WP_011003186.1 3661295..3663601(-) (priA) [Ralstonia pseudosolanacearum strain RUN3223]
ATGACCGCTACCCGTGTTGATGCCGCTCCGCCCGCCGTTGCCCAGGCAGCGCGCGCGGGCGCCATTGCGCGGGTGGTGAT
CGATACGCCGCTGGATGCCGTATTCGACTATCGCTGCGCGCAGCCGGTCGTGCCGGGCCAGCTGGTGGTGGTGCCGTTCG
GCAGCCGGCGCGTGGTGGCGCTGGTGGTGGAGAGCGCCGGCACCACCGAGGTGCCGGCAGACCGCCTGCGCGATGTCGAG
CGCGTGCTCGACTGGGTGCCGCCGCTGGATGCCGAATGGCGCGCGCTGGCCGAATTCGCCGCGGGCTACTACCACCGGGC
GCTGGGTGAAGTGACGCTGCCGGCGCTGCCGCCGTTGTGGCGTACGCCCGGCAGCTGGGACAGCCTGGCCCGCCATGCCA
CCGAGACCGTCTACGTGATGCGCGAGCCGGCGCGCGCCGCCTTGCTCGACAGCATCCCCCGGCGGGCGGCCGGGGCGATG
CGCCTGGCCGAAGCGCTGACGGGCGACGGCGAACTGTCCGCCAGCGCCGCTGTCGCGCTGCACGGGCAGGCGACGGCCAA
GCTGCGCGATTGGGCCGCGGTGGGCTGGGTCGGGGCGGAACTGCGTCCCAGGCCGTTGCTGCCGGTGCCGCGCGATCTGC
CGCCGGCGTCCGTCGCGCCGGCGCTCAATGCCGAGCAGGCTGCTGCCGTCGAGGCCATCGCGCAGGCCGGGGCGGGCGGC
GGTTTCGCGCCGTTCCTGCTGTACGGGGTCACCGGCAGCGGCAAGACCGAGGTCTACCTGCACGCCATCGCCGATGCCCT
CGCGCGCGATCCGGCCGCGCAGGTGCTGATGCTGGTGCCCGAGATCAACCTGACGCCGCAGCTGGAGGCGCGCATTGCGG
CGCGCTTCCCCGGCGTGCCGCTGGCCGCGCTGCACAGCGGCCTGGCCGAGCAGCCGCGCGCGCTGAACTGGCTGGCGGCG
CATCGCGGCGAGGCGCGCATCGTGCTCGGCACGCGGCTGGCGATGCTGGCCTCGCTGCCGCACCTCGCGCTGATCCTCGT
CGACGAGGAGCACGACACGTCCTATAAGCAGCAAGAGGGCCTGCGCTATTCCGCGCGCGATCTGGCGCTGTGGCGGGCCA
AGCAGCGCGGCATCCCGGTCGTGCTCGGGTCGGCCACGCCGTCGCTGGAGACCTGGTGGCGCGCCGAGCAGGGCACCACC
GCGCGCCTGGTGCTGTCGCAGCGCGCGCAGGCCGATGCGGTGCTGCCGCGCGTCTCGCTGATCGACCTGACGCTGGAGCG
CCGCGCGGGCCGGGAGATCCGCGACGGGCTGTCCAGGCCGCTGCTCGATGCCGTTGCCGGGCGCCTGGCGCGCGGCGAGC
AGAGCCTGCTGTTCCTCAACCGCCGCGGCTACGCGCCGGTGCTGGCGTGCGACGCCTGCGGCTGGTTGTCGGGCTGCCCG
CGCTGCTCGGCGTATCTGGTGCTGCACAAGCCGGAGCACCGGCTGCGCTGCCACCACTGCGGCTACGAATCGCGCATCCC
GCACCATTGCCCGGACTGCGGCAACGTCGACATCGCGCCGCTGGGGCGCGGCACGCAGCGCATCGAGGAGACCCTCGGCG
CGCTGTTTCCGCAGGCGCGCGTGGCGCGCATCGACGCCGACTCCACCCGCCGCAAGGGCAGCGCCGAGGCGCTGTTCGAC
ACCGTGCACGAAGGGGCCGTCGATATCCTGATCGGCACGCAGATGGTCGCCAAGGGGCACGACTTTCAGCGCGTGACGCT
GGTGGGCGTGGTGGCGCCCGATGCGTCCCTGTTCAGCCACGACTTCCGTGCCGGCGAGCACCTGTTCGCCTCGCTGATGC
AGGTGGCCGGGCGCGCCGGCCGTGCCGGGCTGGCGGGCGAAGTGCTGATCCAGACCCGCTACCCGGATGCCCCCGCCCTG
CTGGCGCTGGTGCGGCACGATTACGCCGGCTTCGCGCGCCAGCTGCTGCGCGAGCGCAAGCAGGCCTGCCTGCCGCCGTT
CGCCTACCAGGCGCTGCTGACCGCCGAGCACCGCGAGTTGGCGCGCGCGCTGGAATTCCTCGGCGCCGCGCGCGCGGCCG
GGCAGGCCGTGGCCGAAGCCCAGAGCCTGCCGGTGCAACTGCACGATCCGGTGCCCATGACCATGGTGCGCCTGGCCAAC
CGCGAACGCGCCCAACTGCTGCTCGAAAGCGCTTCGCGCGCCGCCCTGCAGCGGCTGCTGGCCACCTGGACCGACGGGTT
TGGCGACATCGGCCGGACGGTCAAGGGCGTGCGCTGGCAGCTCGAGATCGACCCGCTGCGGATCTGA

Domains

Predicted by InterProScan.

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(479-505)

DEAD/DEAH box helicase (DEAD)

(226-388)

3DNA-binding domain (3BD) (PriA_3primeBD)

(24-117)

Primosomal protein N C-terminal domain (PriA_C)

(666-765)

Helicase conserved C-terminal domain (Helicase_C)

(539-623)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8XU91

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

39.333

97.656

0.384