Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   ACEPM4_RS16415 Genome accession   NZ_CP168733
Coordinates   3507334..3509640 (-) Length   768 a.a.
NCBI ID   WP_021154647.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RUN5935     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3502334..3514640
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEPM4_RS16410 (ACEPM4_16410) putA 3503195..3507172 (-) 3978 WP_405124979.1 trifunctional transcriptional regulator/proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase -
  ACEPM4_RS16415 (ACEPM4_16415) priA 3507334..3509640 (-) 2307 WP_021154647.1 primosomal protein N' Machinery gene
  ACEPM4_RS16420 (ACEPM4_16420) hemE 3510223..3511317 (-) 1095 WP_405124980.1 uroporphyrinogen decarboxylase -
  ACEPM4_RS16425 (ACEPM4_16425) - 3511459..3511929 (-) 471 WP_028853829.1 cupin domain-containing protein -
  ACEPM4_RS16430 (ACEPM4_16430) - 3512094..3512441 (+) 348 WP_011003189.1 GFA family protein -
  ACEPM4_RS16435 (ACEPM4_16435) - 3512470..3512850 (+) 381 WP_028853830.1 hypothetical protein -

Sequence

Protein


Download         Length: 768 a.a.        Molecular weight: 82886.34 Da        Isoelectric Point: 8.2063

>NTDB_id=936803 ACEPM4_RS16415 WP_021154647.1 3507334..3509640(-) (priA) [Ralstonia pseudosolanacearum strain RUN5935]
MTATRVDAAPPAVAQAARAGAIARVVIDTPLDAVFDYRCAQPVVPGQLVVVPFGSRRVVALVVETAGTTEVPEDRLRDVE
RVLDWVPPLDAEWRALAEFAAGYYHRALGEVTLPALPPLWRTPGSWDSLARHATETVYVMREPARAALLDSIPRRAAGAM
RLAEALTGDGELSASAAVALHGQATAKLRDWAAVGWVGAELRPRPLLPVPRDLPPASVAPALNAEQAAAVEAIAQAGAGG
GFAPFLLYGVTGSGKTEVYLHAIADALARDPAAQVLMLVPEINLTPQLEARIAARFPGVPLAALHSGLAEQPRALNWLAA
HRGEARIVLGTRLAMLASLPHLALILVDEEHDTSYKQQEGLRYSARDLALWRAKQRGIPVVLGSATPSLETWWRAEQGTT
ARLVLSQRAQADAVLPRVSLIDLTLERRAGREIRDGLSRPLLDAVAGRLARGEQSLLFLNRRGYAPVLACDACGWLSGCP
RCSAYLVLHKPEHRLRCHHCGYESRIPHHCPDCGNVDIAPLGRGTQRIEETLGALFPQARVARIDADSTRRKGSAEALFD
TVHEGAVDILIGTQMVAKGHDFQRVTLVGVVAPDASLFSHDFRAGEHLFASLMQVAGRAGRAGLAGEVLIQTRYPDAPAL
LALVRHDYAGFARQLLRERKQACLPPFAYQALLTAEHRELARALEFLGAARAAGQAVAEAQGLPVQLHDPVPMTMVRLAN
RERAQLLLESASRAALQRLLATWTDGFGDIGRTVKGVRWQLEIDPLRI

Nucleotide


Download         Length: 2307 bp        

>NTDB_id=936803 ACEPM4_RS16415 WP_021154647.1 3507334..3509640(-) (priA) [Ralstonia pseudosolanacearum strain RUN5935]
ATGACCGCTACCCGTGTTGATGCCGCTCCGCCCGCCGTTGCCCAGGCAGCGCGCGCGGGCGCCATTGCGCGGGTGGTGAT
CGATACGCCGCTGGATGCCGTATTCGACTATCGCTGCGCGCAGCCGGTCGTGCCGGGCCAGCTGGTGGTGGTGCCGTTCG
GCAGCCGGCGCGTGGTGGCGCTGGTGGTGGAGACCGCCGGCACCACCGAGGTGCCGGAAGACCGCCTGCGCGATGTCGAG
CGCGTGCTCGACTGGGTGCCGCCGCTGGATGCCGAGTGGCGCGCGCTGGCCGAATTCGCCGCGGGCTACTACCACCGGGC
GCTGGGTGAAGTGACGCTGCCGGCGCTGCCGCCGTTGTGGCGTACGCCCGGCAGCTGGGACAGCCTGGCCCGCCACGCCA
CCGAGACCGTCTACGTGATGCGCGAGCCGGCGCGCGCCGCCTTGCTCGACAGCATCCCCCGGCGGGCGGCCGGGGCGATG
CGCCTGGCCGAAGCGCTGACGGGCGACGGCGAACTGTCCGCCAGCGCCGCTGTCGCGCTGCACGGGCAGGCGACGGCCAA
GCTGCGCGATTGGGCCGCGGTGGGCTGGGTCGGGGCGGAGCTGCGTCCCAGGCCGTTGCTGCCGGTGCCGCGCGATCTGC
CGCCGGCGTCCGTCGCGCCGGCGCTCAATGCCGAGCAGGCCGCTGCCGTCGAGGCCATCGCGCAGGCCGGGGCGGGCGGC
GGTTTCGCGCCGTTCCTGCTGTATGGGGTCACCGGCAGCGGCAAGACCGAGGTCTACCTGCACGCCATCGCCGATGCCCT
CGCGCGCGATCCGGCCGCGCAGGTGCTGATGCTGGTGCCCGAGATCAACCTGACGCCGCAGCTGGAGGCGCGCATTGCGG
CGCGCTTCCCCGGCGTGCCGCTGGCCGCGCTGCACAGCGGCCTGGCCGAGCAGCCGCGTGCGCTGAACTGGCTGGCGGCG
CATCGCGGCGAGGCGCGCATCGTGCTCGGCACGCGGCTGGCGATGCTGGCCTCGCTGCCGCACCTCGCGCTGATCCTCGT
CGACGAGGAGCACGACACGTCCTACAAGCAGCAAGAGGGCCTGCGCTATTCCGCGCGCGATCTGGCGCTGTGGCGGGCCA
AGCAGCGCGGCATCCCGGTCGTGCTCGGGTCGGCCACGCCGTCGCTGGAGACCTGGTGGCGCGCCGAGCAGGGCACCACC
GCGCGCCTGGTGCTGTCGCAGCGCGCGCAGGCCGATGCGGTGCTGCCGCGCGTCTCGCTGATCGACCTGACGCTGGAGCG
CCGCGCGGGCCGGGAGATCCGCGACGGGCTGTCCAGGCCGCTGCTCGATGCCGTTGCCGGGCGCCTGGCGCGCGGCGAGC
AGAGCCTGCTGTTCCTCAACCGCCGCGGCTACGCGCCGGTGCTGGCGTGCGACGCCTGCGGCTGGTTGTCAGGCTGCCCG
CGCTGCTCGGCGTATCTGGTGCTGCACAAGCCGGAGCACCGGCTGCGCTGCCACCACTGCGGCTACGAATCGCGCATCCC
GCACCATTGCCCGGACTGCGGCAACGTCGACATCGCGCCGCTGGGGCGCGGCACGCAGCGCATCGAGGAGACCCTCGGCG
CGCTGTTTCCGCAGGCGCGCGTGGCGCGTATCGACGCCGACTCCACCCGCCGCAAGGGCAGCGCCGAGGCGCTGTTCGAC
ACCGTGCACGAAGGGGCCGTCGATATCCTGATCGGCACGCAGATGGTCGCCAAGGGGCATGACTTCCAGCGCGTGACGCT
GGTGGGCGTGGTGGCGCCCGATGCGTCCCTGTTCAGCCACGACTTCCGTGCCGGCGAGCACCTGTTCGCCTCGCTGATGC
AGGTGGCCGGGCGCGCCGGCCGCGCCGGGCTGGCGGGCGAAGTGCTGATCCAGACCCGCTACCCGGATGCCCCCGCCCTG
CTGGCGCTGGTGCGGCACGATTACGCCGGCTTCGCGCGCCAGCTGCTGCGCGAGCGCAAGCAGGCCTGCCTGCCGCCGTT
CGCCTACCAGGCGCTGCTGACCGCCGAGCACCGCGAGTTGGCGCGCGCGCTGGAATTCCTCGGCGCCGCGCGCGCGGCCG
GGCAGGCCGTGGCCGAAGCCCAGGGCCTGCCGGTGCAACTGCACGATCCGGTGCCCATGACCATGGTGCGCCTGGCCAAC
CGCGAACGCGCCCAACTGCTGCTCGAAAGCGCTTCGCGCGCCGCCCTGCAGCGGCTGCTGGCCACCTGGACCGACGGGTT
TGGCGACATCGGCCGGACGGTCAAGGGCGTGCGCTGGCAGCTCGAGATCGACCCGCTGCGGATCTGA

Domains

Predicted by InterProScan.

3DNA-binding domain (3BD) (PriA_3primeBD)

(24-117)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(479-505)

Primosomal protein N C-terminal domain (PriA_C)

(666-765)

DEAD/DEAH box helicase (DEAD)

(226-388)

Helicase conserved C-terminal domain (Helicase_C)

(539-623)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

39.467

97.656

0.385