Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilK   Type   Machinery gene
Locus tag   V5G12_RS02680 Genome accession   NZ_CP145021
Coordinates   517859..518470 (+) Length   203 a.a.
NCBI ID   WP_012503481.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae strain WHO_V_2024     
Function   type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 519015..556904 517859..518470 flank 545


Gene organization within MGE regions


Location: 517859..556904
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V5G12_RS02680 (V5G12_02685) pilK 517859..518470 (+) 612 WP_012503481.1 PilX N-terminal domain-containing pilus assembly protein Machinery gene
  V5G12_RS02685 (V5G12_02690) pilL 518472..518945 (+) 474 WP_012503482.1 PilX family type IV pilin Machinery gene
  V5G12_RS02690 (V5G12_02695) - 519015..519323 (-) 309 WP_003706588.1 AzlD family protein -
  V5G12_RS02695 (V5G12_02700) - 519320..520031 (-) 712 Protein_521 AzlC family ABC transporter permease -
  V5G12_RS02700 (V5G12_02705) dut 520197..520649 (+) 453 WP_003701071.1 dUTP diphosphatase -
  V5G12_RS02705 (V5G12_02710) dapC 520721..521908 (+) 1188 WP_003701073.1 succinyldiaminopimelate transaminase -
  V5G12_RS02710 (V5G12_02715) yaaA 522064..522843 (+) 780 WP_003687925.1 peroxide stress protein YaaA -
  V5G12_RS02725 (V5G12_02730) - 523373..524573 (+) 1201 Protein_525 tyrosine-type recombinase/integrase -
  V5G12_RS02730 (V5G12_02735) - 524929..525198 (-) 270 WP_003687928.1 hypothetical protein -
  V5G12_RS02735 (V5G12_02740) - 525393..526076 (-) 684 WP_003687929.1 DUF2786 domain-containing protein -
  V5G12_RS02740 (V5G12_02745) - 526390..526623 (-) 234 Protein_528 hypothetical protein -
  V5G12_RS02745 (V5G12_02750) - 526734..526949 (-) 216 WP_003691538.1 hypothetical protein -
  V5G12_RS02750 (V5G12_02755) - 527001..527492 (-) 492 WP_017147227.1 siphovirus Gp157 family protein -
  V5G12_RS02755 (V5G12_02760) - 527489..527671 (-) 183 WP_003691535.1 hypothetical protein -
  V5G12_RS02760 (V5G12_02765) - 527811..528497 (-) 687 WP_042758540.1 phage replication initiation protein, NGO0469 family -
  V5G12_RS02765 (V5G12_02770) - 528566..528727 (-) 162 WP_003691530.1 hypothetical protein -
  V5G12_RS02770 (V5G12_02775) - 528724..528999 (-) 276 WP_003703838.1 NGO1622 family putative holin -
  V5G12_RS02775 (V5G12_02780) - 529152..529484 (-) 333 WP_003687946.1 hypothetical protein -
  V5G12_RS02780 (V5G12_02785) - 529625..529912 (-) 288 WP_115095409.1 hypothetical protein -
  V5G12_RS02785 (V5G12_02790) - 529909..530385 (-) 477 WP_012504141.1 hypothetical protein -
  V5G12_RS02790 (V5G12_02795) - 530418..530618 (-) 201 WP_003704298.1 hypothetical protein -
  V5G12_RS02795 (V5G12_02800) - 530816..531229 (-) 414 WP_003687963.1 hypothetical protein -
  V5G12_RS02800 (V5G12_02805) - 531226..531687 (-) 462 WP_003687965.1 helix-turn-helix domain-containing protein -
  V5G12_RS02805 (V5G12_02810) - 531704..532141 (-) 438 WP_003687967.1 hypothetical protein -
  V5G12_RS02810 (V5G12_02815) - 532256..532972 (-) 717 WP_003687969.1 LexA family transcriptional regulator -
  V5G12_RS02815 (V5G12_02820) - 533041..533277 (+) 237 WP_003687971.1 Cro/CI family transcriptional regulator -
  V5G12_RS02820 (V5G12_02825) - 533357..533512 (+) 156 WP_003689578.1 hypothetical protein -
  V5G12_RS02825 (V5G12_02830) - 533489..533677 (-) 189 WP_003698903.1 hypothetical protein -
  V5G12_RS02830 (V5G12_02835) - 533850..534077 (+) 228 WP_050169780.1 helix-turn-helix domain-containing protein -
  V5G12_RS02835 (V5G12_02840) - 534074..535114 (+) 1041 WP_115095410.1 helix-turn-helix domain-containing protein -
  V5G12_RS02840 (V5G12_02845) - 535130..535912 (+) 783 WP_033910919.1 ATP-binding protein -
  V5G12_RS02845 (V5G12_02850) - 535905..536150 (+) 246 WP_115095411.1 hypothetical protein -
  V5G12_RS02850 (V5G12_02855) - 536226..536720 (+) 495 WP_047923242.1 DUF3310 domain-containing protein -
  V5G12_RS02855 (V5G12_02860) - 536895..537044 (+) 150 WP_041421445.1 hypothetical protein -
  V5G12_RS02860 (V5G12_02865) - 537073..537354 (+) 282 WP_003689109.1 hypothetical protein -
  V5G12_RS02865 (V5G12_02870) - 537345..537782 (+) 438 WP_229701788.1 RusA family crossover junction endodeoxyribonuclease -
  V5G12_RS02870 (V5G12_02875) - 537775..538080 (+) 306 WP_003687981.1 nuclease domain-containing protein -
  V5G12_RS02875 (V5G12_02880) - 538077..538460 (+) 384 WP_003690918.1 recombination protein NinB -
  V5G12_RS02880 (V5G12_02885) - 538451..538969 (+) 519 WP_003693459.1 HNH endonuclease -
  V5G12_RS02885 (V5G12_02890) - 539034..539456 (+) 423 WP_003690919.1 hypothetical protein -
  V5G12_RS02890 (V5G12_02895) - 539456..539995 (+) 540 WP_003690920.1 hypothetical protein -
  V5G12_RS02895 (V5G12_02900) - 539976..541250 (+) 1275 WP_047916940.1 PBSX family phage terminase large subunit -
  V5G12_RS02900 (V5G12_02905) - 541235..543502 (+) 2268 WP_228841300.1 hypothetical protein -
  V5G12_RS02905 (V5G12_02910) - 543740..544936 (+) 1197 WP_003687992.1 hypothetical protein -
  V5G12_RS02910 (V5G12_02915) - 544933..546147 (+) 1215 WP_044270986.1 hypothetical protein -
  V5G12_RS02915 (V5G12_02920) - 549041..552145 (+) 3105 Protein_563 PLxRFG domain-containing protein -
  V5G12_RS02920 (V5G12_02925) - 552127..552864 (+) 738 WP_003690932.1 hypothetical protein -
  V5G12_RS02925 (V5G12_02930) - 552885..554180 (+) 1296 WP_003690933.1 DUF4043 family protein -
  V5G12_RS02930 (V5G12_02935) - 554235..554708 (+) 474 WP_003690936.1 hypothetical protein -
  V5G12_RS02935 (V5G12_02940) - 554714..555199 (+) 486 WP_003687997.1 hypothetical protein -
  V5G12_RS02940 (V5G12_02945) - 555196..555870 (+) 675 WP_003687998.1 hypothetical protein -
  V5G12_RS02945 (V5G12_02950) - 555861..556022 (+) 162 WP_003687999.1 hypothetical protein -
  V5G12_RS02950 (V5G12_02955) - 556059..556904 (-) 846 WP_012503500.1 Bro-N domain-containing protein -

Sequence

Protein


Download         Length: 203 a.a.        Molecular weight: 22021.91 Da        Isoelectric Point: 8.0862

>NTDB_id=935972 V5G12_RS02680 WP_012503481.1 517859..518470(+) (pilK) [Neisseria gonorrhoeae strain WHO_V_2024]
MRKQNTLTGIPTSDGQRGSALFIVLMVMIVVAFLVVTAAQSYNTEQRISANESDRKLALSLAEAALREGEFQVLDLEYAA
DSKVTFSENCEKGLCTAVNVRTNDNGNEEAFGNIVVQGKPTVEAVKRSCPAKSGKNSTGLCIDNQGVEYKKGTGNVSKMP
RYIIEYLGVKNGQNVYRVTAKAWGKNANTVVVLQSYVGNNDEQ

Nucleotide


Download         Length: 612 bp        

>NTDB_id=935972 V5G12_RS02680 WP_012503481.1 517859..518470(+) (pilK) [Neisseria gonorrhoeae strain WHO_V_2024]
ATGCGCAAACAGAACACTTTGACAGGAATCCCGACTTCTGACGGACAGAGGGGGTCCGCACTGTTTATCGTGCTGATGGT
GATGATAGTCGTGGCCTTTTTGGTTGTAACTGCCGCCCAGTCCTACAATACCGAACAGAGGATCAGTGCCAACGAATCAG
ACAGGAAATTGGCTTTGTCTTTAGCCGAGGCGGCTTTGCGGGAGGGCGAATTTCAGGTTTTGGATTTGGAATATGCTGCG
GACAGTAAGGTTACGTTTAGCGAAAACTGTGAAAAAGGTCTGTGTACCGCAGTGAATGTGCGGACAAATGATAATGGTAA
TGAAGAGGCTTTTGGCAATATCGTGGTGCAAGGCAAGCCCACCGTTGAGGCGGTGAAGCGTTCTTGCCCTGCAAAGTCTG
GCAAAAATTCTACCGGCCTGTGCATTGACAATCAGGGAGTGGAATATAAGAAAGGTACGGGAAACGTCAGCAAAATGCCG
CGCTATATTATCGAATATTTAGGCGTGAAGAACGGACAAAATGTTTATCGGGTTACTGCCAAGGCTTGGGGTAAGAATGC
CAATACCGTGGTCGTCCTTCAATCTTATGTAGGCAATAATGATGAGCAATAA

Domains

Predicted by InterproScan.

PilX N-terminal (PilX_N)

(17-67)

Type IV pilus assembly protein PilX C-term (PilX)

(92-198)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilK Neisseria gonorrhoeae MS11

96.059

100

0.961