Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   V5G20_RS00025 Genome accession   NZ_CP144922
Coordinates   3337..4707 (-) Length   456 a.a.
NCBI ID   WP_003392047.1    Uniprot ID   M8DAG7
Organism   Brevibacillus borstelensis strain CP19     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1..9707
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V5G20_RS00010 (V5G20_00010) - 920..1315 (+) 396 WP_003392040.1 hypothetical protein -
  V5G20_RS00015 (V5G20_00015) pssA 1407..2135 (-) 729 WP_003392042.1 CDP-diacylglycerol--serine O-phosphatidyltransferase -
  V5G20_RS00020 (V5G20_00020) disA 2250..3326 (-) 1077 WP_003392045.1 DNA integrity scanning diadenylate cyclase DisA -
  V5G20_RS00025 (V5G20_00025) radA 3337..4707 (-) 1371 WP_003392047.1 DNA repair protein RadA Machinery gene
  V5G20_RS00030 (V5G20_00030) - 4828..7281 (-) 2454 WP_003392048.1 ATP-dependent Clp protease ATP-binding subunit -
  V5G20_RS00035 (V5G20_00035) - 7377..8441 (-) 1065 WP_003392050.1 protein arginine kinase -
  V5G20_RS00040 (V5G20_00040) - 8460..8978 (-) 519 WP_338462905.1 UvrB/UvrC motif-containing protein -
  V5G20_RS00045 (V5G20_00045) - 9007..9471 (-) 465 WP_003392054.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49248.73 Da        Isoelectric Point: 6.7257

>NTDB_id=935279 V5G20_RS00025 WP_003392047.1 3337..4707(-) (radA) [Brevibacillus borstelensis strain CP19]
MSKYKTKYACQECGYESPKWMGKCPGCNNWNTMVEEVAVKTASRHESLRGGQRQTAALPLTEVAGEEEPRMDTTIGELNR
VLGGGLVPGSMVLVGGDPGIGKSTLLLQTSFALAHGGIKVLYVSGEESAKQIKLRADRLGMSTPPMYVLAENDLELIEQH
IAQVDPQVLIIDSIQTVFHPAVQSAAGSVAQVRETTSQLMRIAKGKGIATFIVGHVTKEGAIAGPRMLEHMVDAVLYFEG
ERHNTFRILRAVKNRFGSTNEIGIFEMKEKGLEEVGNPSEIFLAERPVGVAGSTVVASMEGTRPVLVELQALVAPTSFVT
PRRMATGVDHQRVGMIMAVLEKRMGLMLQNQDAYVNVAGGVRLDEPAVDLAIAVSIASSFRDHATNPHDVVIGEVGLTGE
VRGVSRIEQRIREAHKLGFKRVIIPSKNIRGLDVPSDIEVVGVSNISEALSEVLRG

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=935279 V5G20_RS00025 WP_003392047.1 3337..4707(-) (radA) [Brevibacillus borstelensis strain CP19]
ATGTCCAAGTATAAAACGAAATACGCGTGTCAAGAATGTGGCTATGAATCGCCCAAATGGATGGGGAAATGTCCTGGCTG
CAACAATTGGAATACGATGGTGGAAGAGGTTGCGGTAAAGACCGCCTCCCGTCATGAGAGTTTGCGTGGCGGACAGCGGC
AAACAGCAGCCCTTCCGCTGACAGAGGTGGCCGGCGAAGAAGAGCCGCGGATGGATACAACGATAGGTGAATTAAACCGG
GTTCTGGGCGGAGGGCTCGTTCCCGGCTCAATGGTTCTGGTCGGCGGAGACCCGGGGATCGGGAAGTCTACCTTGCTGTT
GCAAACGTCGTTCGCGCTTGCACACGGGGGAATAAAGGTCTTGTACGTATCCGGCGAGGAGTCCGCCAAGCAAATCAAAT
TGCGTGCCGATCGGCTCGGAATGAGCACGCCGCCGATGTATGTGCTTGCAGAAAACGATTTGGAGCTTATTGAGCAGCAC
ATTGCCCAGGTCGATCCCCAGGTCCTGATCATCGACTCGATTCAGACTGTCTTCCATCCCGCTGTCCAATCAGCGGCGGG
GAGTGTCGCACAGGTGAGGGAAACAACCTCTCAACTGATGCGAATCGCCAAGGGAAAAGGAATCGCAACTTTTATCGTCG
GTCACGTGACGAAGGAAGGCGCGATTGCCGGGCCCCGCATGCTGGAGCATATGGTGGATGCGGTCTTGTACTTTGAAGGG
GAACGGCATAATACGTTCCGCATTTTGCGAGCAGTGAAAAACCGCTTTGGCTCGACGAATGAAATCGGCATCTTTGAGAT
GAAAGAAAAGGGGCTCGAGGAAGTTGGAAACCCTTCGGAAATCTTTTTGGCAGAACGTCCGGTCGGCGTCGCTGGCTCGA
CTGTAGTGGCCAGCATGGAGGGAACCAGGCCGGTTCTGGTAGAGCTTCAGGCGCTGGTTGCACCGACCAGCTTTGTGACC
CCGCGTCGGATGGCTACCGGCGTGGATCACCAGCGAGTCGGCATGATCATGGCAGTGCTTGAGAAGCGCATGGGTCTCAT
GCTGCAAAATCAGGATGCCTATGTCAACGTGGCTGGCGGTGTCCGTTTGGATGAGCCCGCTGTCGATTTGGCGATAGCTG
TCAGCATTGCCAGCAGCTTTCGCGATCACGCGACCAATCCCCATGATGTCGTGATCGGGGAAGTGGGATTAACGGGTGAA
GTCCGCGGCGTGTCCCGGATTGAACAGCGGATTCGCGAAGCGCACAAGCTAGGATTCAAACGGGTGATCATCCCGTCCAA
AAATATCCGCGGTCTTGACGTTCCATCGGACATCGAGGTAGTCGGTGTAAGCAATATTTCGGAAGCATTAAGCGAAGTGC
TAAGGGGGTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(342-428)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M8DAG7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.939

100

0.662
  radA Streptococcus pneumoniae Rx1

58.462

99.781

0.583
  radA Streptococcus pneumoniae D39

58.462

99.781

0.583
  radA Streptococcus pneumoniae R6

58.462

99.781

0.583
  radA Streptococcus pneumoniae TIGR4

58.462

99.781

0.583
  radA Streptococcus mitis NCTC 12261

58.15

99.561

0.579
  radA Streptococcus mitis SK321

58.15

99.561

0.579