Detailed information NTG0934431

Bioinformatically predicted

Overview

• Name: pilF
• Type: Machinery gene
• Locus tag: V4943_RS08330
• Genome accession: NZ_CP144685
• Coordinates: 1542880..1545549 (-)
• Length: 889 a.a.
• NCBI ID: WP_182223925.1
• Uniprot ID: -
• Organism: Thermus thermophilus strain HB27_delta_csm3
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1537880..1550549

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V4943_RS08310 (V4943_08310)	-	1538726..1539358 (-)	633	WP_011173987.1	histidine phosphatase family protein	-
V4943_RS08315 (V4943_08315)	purM	1539355..1540356 (-)	1002	WP_011173988.1	phosphoribosylformylglycinamidine cyclo-ligase	-
V4943_RS08320 (V4943_08320)	gatB	1540362..1541771 (-)	1410	WP_011173989.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB	-
V4943_RS08325 (V4943_08325)	pilT	1541782..1542867 (-)	1086	WP_011173990.1	type IV pilus twitching motility protein PilT	Machinery gene
V4943_RS08330 (V4943_08330)	pilF	1542880..1545549 (-)	2670	WP_182223925.1	type IV pilus assembly ATPase PilB	Machinery gene
V4943_RS08335 (V4943_08335)	-	1545546..1546031 (-)	486	WP_011173992.1	YqeG family HAD IIIA-type phosphatase	-
V4943_RS08340 (V4943_08340)	pgeF	1546066..1546794 (-)	729	WP_011173993.1	peptidoglycan editing factor PgeF	-
V4943_RS08345 (V4943_08345)	surE	1546791..1547546 (-)	756	WP_011173994.1	5'/3'-nucleotidase SurE	-
V4943_RS08350 (V4943_08350)	-	1547605..1547811 (-)	207	WP_011173995.1	cold-shock protein	-
V4943_RS08355 (V4943_08355)	-	1547969..1549258 (+)	1290	WP_011173996.1	ABC transporter substrate-binding protein	-
V4943_RS08360 (V4943_08360)	-	1549310..1550185 (+)	876	WP_011173997.1	sugar ABC transporter permease	-

Sequence

Protein

• Length: 889 a.a.
• Molecular weight: 98059.12 Da
• Isoelectric Point: 5.2731

>NTDB_id=934431 V4943_RS08330 WP_182223925.1 1542880..1545549(-) (pilF) [Thermus thermophilus strain HB27_delta_csm3]
MSVLTIGDKRLGAALLDAGLLTDEELQRALERHREVGGSLAEVLVDMGLLSERRIAQTIEDRFGIPLVELHRVEIPPKVK
ALLPAEKAKELKAIPFALDEEAGVVRVAFLNPLDTLSLEEVEDLTGLVVEPYQTTKSAFLYALAKHYPELGLPVPPPPSG
EGQKDLKLGELLLQKGWISREALEEALVEQEKTGDLLGRILVRKGLPEEALYRALAEQKGLEFLESTEGIVPDPSAALLL
LRSDALRYGAVPIGFQNGEVEVVLSDPRHKEAVAQLLNRPARFYLALPQAWEELFGRAYPEKNRLGEVLVQEGKLSREAL
KEALEVQKGLPRAKPLGEILVELGLARPEDVEEALQKQRRGGGRLEDTLVQSGKLRPEALAQAVATQLGYPYVDPEEDPP
DPGAPLLLPEDLCRRYGVFPHRLEGNRLVLLMKDPRNILALDDVRLALKRKGLNHEVAPAVATEAAITKLIERFYGKAEL
SEIAKEFAQKQVVEEAPSPLELDESAAQKFVKQVIREAFLQDASDIHIEPRQNDVQVRLRIDGALRPYSTLPKGALNAVI
SVVKIMGGLNIAEKRLPQDGRVRYREGAIDVDLRLSTLPTVYGEKAVMRLLKKASDIPEIEDLGFAPGVFERFKEVISKP
YGIFLITGPTGSGKSFTTFSILKRIATPDKNTQTIEDPVEYEIPGINQTQVNPQAGLTFARALRAFLRQDPDIIMVGEIR
DSETAKIATEAALTGHLVIATLHTNDAAQAITRLDEMGVEPFNISAALIGVLSQRLVRRVCEHCKVEVKPDPETLRRLGL
SEAEIQGARLYKGMGCERCGGTGYKGRYAIHELLVVDDEIRHAIVAGKSATEIKEIARRKGMKTLREDGLYKALQGITTL
EEVLARTIE

Nucleotide

• Length: 2670 bp

>NTDB_id=934431 V4943_RS08330 WP_182223925.1 1542880..1545549(-) (pilF) [Thermus thermophilus strain HB27_delta_csm3]
ATGAGCGTGCTCACCATAGGGGACAAAAGGCTCGGGGCGGCCCTCTTGGACGCCGGGCTCCTCACGGACGAGGAGCTGCA
GCGGGCCTTGGAACGGCACCGGGAGGTGGGGGGCTCCTTGGCCGAGGTCCTGGTGGACATGGGCCTCCTCTCCGAGAGGA
GGATCGCCCAGACCATTGAGGACCGCTTCGGCATCCCCCTGGTGGAGCTCCACCGGGTGGAGATCCCCCCCAAGGTCAAG
GCCCTCCTTCCCGCGGAGAAGGCCAAGGAACTCAAGGCCATCCCCTTCGCCCTGGACGAGGAGGCGGGGGTGGTGCGGGT
GGCCTTCCTCAACCCCCTGGACACCCTGAGCCTCGAGGAGGTGGAGGACCTCACGGGGCTCGTGGTGGAGCCGTACCAGA
CCACCAAAAGCGCCTTCCTCTACGCCCTGGCCAAACACTACCCGGAGCTCGGCCTTCCCGTCCCCCCGCCGCCTTCGGGC
GAGGGCCAGAAGGACCTCAAGCTCGGGGAGCTCCTCCTGCAAAAGGGGTGGATCTCCCGCGAGGCCCTGGAGGAGGCCTT
GGTGGAACAGGAGAAGACGGGGGACCTCCTCGGGCGGATCCTGGTGCGGAAGGGGCTCCCCGAGGAGGCCCTTTACCGGG
CCTTGGCGGAGCAGAAGGGGCTGGAGTTTCTGGAAAGCACCGAGGGGATCGTCCCCGACCCCTCCGCCGCCCTCCTCCTC
CTCCGCTCCGACGCCCTGCGTTACGGCGCCGTGCCCATCGGCTTCCAAAACGGGGAGGTGGAGGTGGTCCTCTCCGACCC
CCGGCACAAGGAGGCGGTGGCCCAGCTCCTCAATCGGCCCGCCCGCTTCTACCTCGCCCTTCCCCAGGCCTGGGAGGAGC
TTTTCGGCCGGGCCTACCCCGAGAAGAACCGCCTGGGGGAGGTCCTGGTCCAGGAGGGCAAGCTCTCCCGGGAAGCGCTG
AAGGAGGCCCTGGAGGTGCAGAAAGGCCTGCCCCGGGCCAAGCCTTTGGGGGAGATCCTGGTGGAGCTCGGCCTCGCCCG
CCCCGAGGACGTGGAGGAGGCCCTGCAGAAGCAAAGGCGGGGCGGGGGCCGGCTGGAGGACACCCTGGTCCAGTCGGGGA
AGCTCAGGCCCGAGGCCCTGGCCCAGGCGGTGGCCACCCAGCTGGGCTACCCCTACGTTGACCCCGAGGAGGACCCCCCC
GATCCCGGCGCCCCCCTCCTCCTCCCAGAGGACCTGTGCCGCCGCTATGGGGTCTTCCCCCACCGCCTCGAGGGGAACCG
CCTCGTCCTCCTGATGAAAGACCCGAGGAACATCCTGGCCCTGGACGACGTGCGCCTCGCCCTCAAGCGGAAGGGCCTGA
ACCACGAGGTGGCCCCGGCGGTGGCCACCGAGGCCGCCATCACCAAGCTCATCGAGCGCTTCTACGGCAAGGCCGAGCTC
TCGGAGATCGCCAAGGAGTTCGCCCAAAAACAGGTGGTGGAAGAGGCTCCAAGTCCCCTGGAGCTGGACGAGAGCGCCGC
CCAGAAGTTCGTGAAGCAGGTGATCCGGGAAGCCTTCCTCCAGGACGCCTCCGACATCCACATCGAGCCCAGGCAGAACG
ACGTCCAGGTGCGCCTAAGGATTGACGGCGCCCTGCGGCCGTACAGCACCCTGCCGAAGGGAGCGCTGAACGCGGTGATC
TCCGTGGTCAAGATCATGGGCGGGCTCAACATCGCCGAGAAGCGCCTCCCCCAGGACGGACGGGTGCGCTACCGGGAAGG
GGCCATAGACGTGGACCTCCGGCTTTCCACCCTGCCCACGGTCTACGGGGAGAAGGCGGTGATGCGCCTCCTCAAGAAGG
CCTCGGACATCCCCGAGATCGAGGACCTGGGCTTCGCCCCGGGGGTGTTTGAACGCTTCAAGGAGGTGATCTCCAAGCCC
TACGGCATCTTCCTCATCACCGGGCCCACGGGGTCGGGCAAGAGCTTCACCACCTTCTCCATCCTCAAGCGCATCGCCAC
CCCCGACAAGAACACCCAGACCATTGAAGACCCCGTGGAGTACGAGATCCCCGGGATCAACCAGACCCAGGTGAACCCCC
AGGCGGGCCTCACCTTCGCCCGGGCGCTAAGGGCCTTCCTCAGGCAGGACCCGGACATCATCATGGTGGGGGAGATCCGG
GACTCCGAGACGGCCAAGATCGCCACCGAAGCCGCCCTCACCGGCCACCTCGTCATCGCCACCCTGCACACCAACGACGC
CGCCCAGGCCATCACCCGCCTGGACGAGATGGGGGTGGAGCCCTTCAACATCTCCGCGGCCCTCATCGGCGTCCTCTCCC
AGCGCCTGGTGCGCAGGGTGTGCGAGCACTGCAAGGTGGAGGTCAAGCCGGACCCCGAGACCCTCAGGCGCCTCGGGCTT
TCCGAGGCGGAGATCCAAGGGGCCAGGCTCTACAAGGGCATGGGGTGCGAGCGGTGCGGCGGCACCGGGTACAAGGGCCG
CTACGCCATCCACGAGCTTTTGGTGGTGGACGACGAGATCCGCCACGCCATCGTGGCGGGGAAGTCGGCCACGGAGATCA
AGGAGATCGCCCGGAGGAAGGGGATGAAGACCCTGAGGGAGGACGGCCTCTACAAGGCCCTCCAAGGGATCACCACCCTC
GAGGAGGTCCTGGCGCGTACCATTGAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (513-779)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (201-302)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (369-479)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (44-148)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilF	Thermus thermophilus HB27	99.213	100	0.992
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	55.955	100	0.56