Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   V4S27_RS00255 Genome accession   NZ_CP144495
Coordinates   55357..56727 (+) Length   456 a.a.
NCBI ID   WP_047333966.1    Uniprot ID   A0A200I045
Organism   Enterococcus cecorum strain 70     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 50357..61727
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V4S27_RS00230 (V4S27_00230) - 51709..52956 (-) 1248 WP_347915129.1 glutamate-5-semialdehyde dehydrogenase -
  V4S27_RS00235 (V4S27_00235) proB 52972..53775 (-) 804 WP_235426847.1 glutamate 5-kinase -
  V4S27_RS00240 (V4S27_00240) - 53909..54073 (-) 165 WP_162607281.1 hypothetical protein -
  V4S27_RS00245 (V4S27_00245) - 54091..54600 (-) 510 WP_322388745.1 hypothetical protein -
  V4S27_RS00250 (V4S27_00250) - 54823..55344 (+) 522 WP_026209954.1 hypothetical protein -
  V4S27_RS00255 (V4S27_00255) radA 55357..56727 (+) 1371 WP_047333966.1 DNA repair protein RadA Machinery gene
  V4S27_RS00260 (V4S27_00260) - 56929..58035 (+) 1107 WP_016250426.1 PIN/TRAM domain-containing protein -
  V4S27_RS00265 (V4S27_00265) rfbC 58075..58632 (+) 558 WP_047241735.1 dTDP-4-dehydrorhamnose 3,5-epimerase -
  V4S27_RS00270 (V4S27_00270) - 58927..59196 (+) 270 WP_243184525.1 hypothetical protein -
  V4S27_RS00275 (V4S27_00275) - 59265..59741 (+) 477 WP_282013347.1 GNAT family N-acetyltransferase -
  V4S27_RS00280 (V4S27_00280) gltX 59836..61293 (+) 1458 WP_243192492.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49967.80 Da        Isoelectric Point: 7.7241

>NTDB_id=934055 V4S27_RS00255 WP_047333966.1 55357..56727(+) (radA) [Enterococcus cecorum strain 70]
MAKKAKTQFECQHCGYISPRYLGKCPNCNSWNSMMEVKIENTTDVRQAVSFTGIKSQPTKITEVVPKKTPRVQTEIGELN
RVLGGGVVPGSLILLGGDPGIGKSTLLLQVSEQLTKAHGKVLYVSGEESAEQIKMRAERLGVLHESFYVYAENDMEAIRQ
AIEQLSPDYVIIDSIQTMIQPSISSVAGSVSQVRETTAELMRIAKSNQIAIFIVGHVTKEGALAGPRMLEHMVDTVLYFE
GERQQSFRILRAVKNRFGSTNEIGIFEMHEDGLHEVINPSQVFLEERLQGATGSAIVVTMEGTRPLLVEIQALITPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGMLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQATDCFIGEIGLTG
EIRRVNAIEQRIKEVQKLGFKRVFVPKNNLANLEIPAGIEVIGVSTLAETMKKVFG

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=934055 V4S27_RS00255 WP_047333966.1 55357..56727(+) (radA) [Enterococcus cecorum strain 70]
GTGGCAAAAAAAGCAAAAACACAATTTGAGTGTCAACATTGTGGCTATATTTCCCCACGTTATTTAGGGAAATGTCCGAA
TTGTAATAGTTGGAACTCGATGATGGAAGTCAAAATCGAAAATACCACCGATGTCCGTCAAGCTGTTTCGTTTACCGGAA
TCAAGAGCCAACCAACCAAAATTACCGAAGTGGTGCCGAAAAAAACACCGCGCGTCCAAACAGAAATTGGTGAGTTGAAT
CGCGTGCTAGGTGGCGGCGTGGTTCCTGGTTCCTTGATTTTACTAGGAGGCGATCCGGGTATTGGCAAGTCGACACTGTT
ATTACAAGTATCCGAGCAGCTAACCAAAGCCCACGGGAAAGTCTTGTACGTCTCAGGGGAAGAAAGTGCTGAGCAAATCA
AGATGCGTGCGGAACGTCTAGGGGTCTTGCATGAGTCCTTTTATGTCTATGCGGAAAATGATATGGAGGCCATTCGTCAA
GCCATCGAACAACTTTCGCCTGATTATGTGATTATTGATTCGATTCAAACCATGATTCAGCCAAGCATTTCTTCTGTGGC
GGGAAGTGTCAGTCAGGTCCGCGAAACCACCGCTGAATTGATGCGCATAGCCAAGAGTAATCAGATTGCCATTTTTATCG
TTGGTCATGTGACAAAAGAAGGGGCACTAGCAGGTCCTAGAATGTTAGAACACATGGTTGATACCGTGTTATATTTTGAA
GGAGAACGCCAACAGTCCTTTAGAATTTTGCGTGCGGTGAAAAATCGTTTCGGTTCGACCAATGAAATCGGCATTTTCGA
GATGCATGAAGACGGCTTGCATGAAGTCATCAATCCTTCGCAAGTCTTTTTAGAAGAACGTCTGCAAGGAGCGACTGGTT
CTGCAATTGTGGTGACGATGGAAGGAACGCGCCCATTATTAGTGGAGATTCAAGCGTTGATTACACCCACCATGTTTGGA
AATGCCAAGCGTACCACGACAGGTCTAGACTTTAATCGGGTATCTTTAATTATGGCGGTCTTGGAAAAGCGTGCCGGAAT
GTTATTGCAAAATCAAGATGCCTATTTAAAAGCAGCCGGTGGTGTGAAGTTAAACGAGCCAGCAATCGATTTAGCCATTG
CGGTGAGTATTGCCTCTAGTTACAAGGAAAAAGGCACCCAGGCAACGGATTGTTTTATCGGTGAAATTGGCTTAACTGGG
GAAATCAGACGCGTCAATGCCATCGAACAACGCATCAAAGAAGTGCAAAAGTTAGGCTTTAAACGGGTATTTGTACCTAA
AAATAATTTAGCTAATTTAGAAATTCCAGCGGGTATTGAAGTTATCGGGGTTTCTACTTTGGCTGAAACGATGAAAAAAG
TCTTTGGCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-221)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A200I045

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

70.769

99.781

0.706
  radA Streptococcus pneumoniae D39

70.769

99.781

0.706
  radA Streptococcus pneumoniae R6

70.769

99.781

0.706
  radA Streptococcus pneumoniae TIGR4

70.769

99.781

0.706
  radA Streptococcus mitis NCTC 12261

70.769

99.781

0.706
  radA Streptococcus mitis SK321

70.769

99.781

0.706
  radA Bacillus subtilis subsp. subtilis str. 168

62.857

99.781

0.627