Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VZ068_RS17810 Genome accession   NZ_CP144460
Coordinates   4250139..4251272 (+) Length   377 a.a.
NCBI ID   WP_259156566.1    Uniprot ID   A0AAU7P6P6
Organism   Xanthomonas sp. 10-10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4245139..4256272
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VZ068_RS17785 (VZ068_17790) - 4245284..4246498 (-) 1215 WP_349656036.1 cystathionine gamma-synthase -
  VZ068_RS17790 (VZ068_17795) - 4246588..4247958 (-) 1371 WP_259156539.1 pyridoxal-phosphate dependent enzyme -
  VZ068_RS17795 (VZ068_17800) - 4248197..4248415 (-) 219 WP_002814034.1 YdcH family protein -
  VZ068_RS17800 (VZ068_17805) - 4248769..4249659 (-) 891 WP_349656037.1 hypothetical protein -
  VZ068_RS17805 (VZ068_17810) - 4249656..4250039 (-) 384 WP_046963332.1 DUF4398 domain-containing protein -
  VZ068_RS17810 (VZ068_17815) pilU 4250139..4251272 (+) 1134 WP_259156566.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VZ068_RS17815 (VZ068_17820) maiA 4252070..4252732 (-) 663 WP_349656038.1 maleylacetoacetate isomerase -
  VZ068_RS17820 (VZ068_17825) - 4252776..4253834 (-) 1059 WP_349657749.1 fumarylacetoacetate hydrolase family protein -
  VZ068_RS17825 (VZ068_17830) - 4254141..4255529 (+) 1389 WP_259156570.1 DEAD/DEAH box helicase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41793.14 Da        Isoelectric Point: 6.1401

>NTDB_id=933965 VZ068_RS17810 WP_259156566.1 4250139..4251272(+) (pilU) [Xanthomonas sp. 10-10]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKGLDGRRRPATEVLLNTPMIRDLLRRGQVHEVKQAMEESLEEGMQTFDQCL
FRMVKSGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYETAAAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=933965 VZ068_RS17810 WP_259156566.1 4250139..4251272(+) (pilU) [Xanthomonas sp. 10-10]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTCTTGACCACCGGTGCGCCGGTGTA
CATCAAGATCGAAGGCAAGCTCTACCCGCTCGGCAATACGGGGTTGCCGCCGGGGATGGTCAAGAAAATCGCTTACTCAT
TGATGGACGAAGGCCAGGTACCGCAGTTCGAGCGCGAGCTCGAGCTCAACATGGCCATCGCCCTGCCCGACGCCGGCCGC
TTCCGCGTGAACGTGTTCAAGCAGCGAGGCGAGGTGGGCATGGTGATCCGCGCCATCCGCAGCCGGATCCCGAGCATCGA
AGAACTCAACCTGCCGCAGGTGCTGAAGGACGTCATCATGACTCCGCGCGGGCTGGTGCTGGTGGTCGGTTCGACCGGCT
CCGGCAAGTCGACCTCGCTGGCCTCGATGATCGACCACCGCAACAGCACCACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAATATCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAGGTGGGCCTGGACACGCATGCGTTCCAGAG
CGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGATGCCGAGACCATGGAGGCGG
CCATCGCCTTCGCCGAGACCGGTCACCTGTGCCTGGCCACCTTGCACTCCAACAATGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAATCTGGCGCTGAATCTGCGCGCAGTGGTCTCGCAGCG
CCTGGTCAAGGGGCTGGATGGGCGCCGTCGCCCGGCGACCGAAGTGCTGCTCAATACCCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTGCACGAGGTCAAGCAGGCGATGGAAGAGTCGCTGGAAGAAGGCATGCAGACCTTCGACCAATGTCTG
TTCCGCATGGTCAAATCAGGGGAAATCGAGCAAGAGGAAGCGCTGCGCGCTGCCGACTCGCGCGATGGCCTGGCACTGAA
GTTCCGGCTGTCCGAAGGTGGTTCGGGCGAGCACGACCCGTATGCGGATTACGAGACTGCCGCCGCACCGAAGATCAGTC
ACGGATTTATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.368

95.225

0.499
  pilU Vibrio cholerae strain A1552

49.167

95.491

0.469
  pilU Acinetobacter baylyi ADP1

49.435

93.899

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.241

100

0.448
  pilT Pseudomonas aeruginosa PAK

42.899

89.655

0.385
  pilT Acinetobacter baumannii strain A118

41.42

89.655

0.371
  pilT Acinetobacter nosocomialis M2

41.42

89.655

0.371
  pilT Acinetobacter baumannii D1279779

41.42

89.655

0.371
  pilT Legionella pneumophila strain Lp02

41.42

89.655

0.371
  pilT Legionella pneumophila strain ERS1305867

41.42

89.655

0.371
  pilT Acinetobacter baylyi ADP1

40.882

90.186

0.369
  pilT Pseudomonas stutzeri DSM 10701

41.317

88.594

0.366
  pilT Vibrio cholerae strain A1552

40.841

88.329

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

88.329

0.361
  pilT Neisseria gonorrhoeae MS11

39.193

92.042

0.361
  pilT Neisseria meningitidis 8013

39.193

92.042

0.361