Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   V4D31_RS02145 Genome accession   NZ_CP144374
Coordinates   428345..429250 (+) Length   301 a.a.
NCBI ID   WP_353686607.1    Uniprot ID   A0AAU8H3Q8
Organism   Thermodesulfovibrio sp. 3462-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 423345..434250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V4D31_RS02120 (V4D31_02100) - 423726..424112 (-) 387 WP_353686602.1 hypothetical protein -
  V4D31_RS02125 (V4D31_02105) mreC 424208..424969 (-) 762 WP_353686603.1 rod shape-determining protein MreC -
  V4D31_RS02130 (V4D31_02110) - 424969..426003 (-) 1035 WP_353686604.1 rod shape-determining protein -
  V4D31_RS02135 (V4D31_02115) - 426051..426458 (-) 408 WP_353686605.1 RDD family protein -
  V4D31_RS02140 (V4D31_02120) - 426600..428345 (+) 1746 WP_353686606.1 hypothetical protein -
  V4D31_RS02145 (V4D31_02125) pilA 428345..429250 (+) 906 WP_353686607.1 signal recognition particle-docking protein FtsY Machinery gene
  V4D31_RS02155 (V4D31_02135) - 429364..429963 (+) 600 WP_353686608.1 XTP/dITP diphosphatase -
  V4D31_RS02160 (V4D31_02140) - 430065..430715 (+) 651 WP_353686609.1 adenylate kinase -
  V4D31_RS02165 (V4D31_02145) sat 430728..432113 (+) 1386 WP_353686610.1 sulfate adenylyltransferase -
  V4D31_RS02170 (V4D31_02150) - 432126..432527 (+) 402 WP_353686611.1 hypothetical protein -
  V4D31_RS02175 (V4D31_02155) aprB 432552..432980 (+) 429 WP_353686612.1 adenylyl-sulfate reductase subunit beta -

Sequence

Protein


Download         Length: 301 a.a.        Molecular weight: 32697.26 Da        Isoelectric Point: 9.6045

>NTDB_id=933875 V4D31_RS02145 WP_353686607.1 428345..429250(+) (pilA) [Thermodesulfovibrio sp. 3462-1]
MGLFDKLKEKLSKTKQHIVEKIEAVVPVGKKIDETTIEEIEEILISSDVGIQATEQITSILRKKVKEGALKDYSDLKSLL
KNELYKILENGTSLNLSSKPAVIMVVGVNGVGKTTTIGKLGYKFISEGKSVVFAAADTFRAAAIQQLEIWANRVGADIVK
HKSGADPAAVVFDAVQHARAKKKDIVIIDTAGRLHTKQPLMEELKKINRVIKKSIPEAPHEILLILDATTGQNAIKQAQL
FNEAIGLTGVVVTKLDGTAKGGVIFAIKKEIGIPVKLIGIGEGIDDLKEFNPKEFVEALFD

Nucleotide


Download         Length: 906 bp        

>NTDB_id=933875 V4D31_RS02145 WP_353686607.1 428345..429250(+) (pilA) [Thermodesulfovibrio sp. 3462-1]
ATGGGTTTATTTGACAAACTCAAGGAAAAATTAAGCAAGACAAAGCAACATATTGTTGAAAAAATTGAGGCTGTTGTTCC
AGTAGGTAAAAAAATTGATGAAACAACAATAGAAGAAATAGAGGAGATTTTAATATCCTCTGATGTTGGTATACAGGCAA
CAGAACAAATTACATCTATTCTAAGAAAAAAAGTAAAAGAAGGAGCATTAAAAGACTACAGCGATCTCAAATCTCTACTT
AAAAATGAGCTTTATAAAATTCTTGAAAACGGAACCAGTCTAAATTTATCCTCAAAACCTGCAGTAATCATGGTTGTTGG
AGTAAATGGAGTTGGAAAAACTACAACTATTGGGAAGCTGGGTTATAAATTCATAAGTGAAGGTAAATCTGTTGTTTTTG
CTGCTGCAGATACCTTTAGAGCTGCAGCTATACAACAGCTTGAAATATGGGCAAACAGAGTAGGAGCTGATATTGTCAAA
CATAAAAGTGGTGCAGACCCAGCTGCTGTTGTTTTTGATGCAGTGCAGCATGCCAGAGCAAAAAAAAAGGACATAGTGAT
TATAGATACAGCAGGAAGACTTCATACAAAGCAACCATTAATGGAAGAGTTAAAAAAAATCAACAGAGTTATAAAAAAAT
CAATTCCTGAAGCACCCCATGAAATTTTGCTCATATTAGATGCTACAACAGGGCAGAATGCTATTAAACAGGCTCAACTT
TTTAATGAAGCTATAGGATTAACAGGAGTTGTAGTAACAAAACTTGATGGAACAGCAAAAGGTGGCGTTATTTTCGCAAT
AAAGAAGGAAATAGGCATTCCTGTTAAATTAATCGGTATAGGCGAAGGAATTGATGATTTAAAGGAGTTTAACCCTAAAG
AGTTTGTTGAAGCTTTGTTTGATTAA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(100-300)

SRP54-type protein, helical bundle domain (SRP54_N)

(20-84)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.355

100

0.488